PHA CH B GAM MU PROTEIN

CN. Nguyn Minh Thi

I/ MC CH V YU CU:
- tin hnh nh lng Protein trong cc dch sinh vt c nng thp ta khng
tin hnh c
trn cc my quang ph theo cc k thut hin hnh.
Ti Bnh Vin Nhi Qung Nam cn tin hnh nh lng nng Protein trong
dch no ty (DNT)
khng thc hin c, v vy cn phi tin hnh nh lng theo phng php so
c khi cho ta
Protein, bng Acid tricloacetid 30% (k thut ca mestreza) ta cn phi c b gam
mu Protein chun
i chiu xc nh kt qu. Hin nay trn th trng khng c bn do ti xin
nghin cu pha ch b
gam mu Protein theo iu kin ca phng xt nghim.
II/ NGUYN TC K THUT (MESTREZA)
- Protein trong dung dch b kt ta bi acid triloacetid 30% theo t l 1/10, nng
Protein t l
thun vi c.
III/ IU KIN TIN HNH
1. Dng c v vt t:
- Dung dch Albumin chun 40g/l
- Dung dch NaCl 0.9%
- Acid Tricloacetid 30%

- Pipep
- Gi ng nghim v ng nghim thy tinh.
- Focmon
- Paraphin v mt s dng c khc
2. K thut tin hnh:
- Tt c cc dng c pha ch v ng nghim thy tinh c hp tit trng v pha
trong iu kin
phng xt nghim trnh tc nhn xm nhp.
- u tin ta pha dd gc Protein 1g/l t dd chun 40g/l theo 2 bc.
+ Trong mt ng nghim ly 1ml dd Protein 40g/l + 3ml NaCl 0.9% ta c 4ml
dd
Protein 10g/l.
+ Trong ng nghim ly 1ml dd Protein 10g/l + 9 ml NaCl 0.9% ta c 10ml dd
Protein
1g/l
tin hnh pha b gam mu ta lp bng nh sau:

Trn 10 ng nghim thy tinh u nhau ta ln lc phn phi cc dd theo bng trn
chnh xc trnh
nhim trng sau nt chc cc ng nghim bng nt cao su dn nhn tng ng
nghim theo nng
Protein c xc lp theo bng cho thm vo mi ng 50 l focmol nhm chng
phn hy Protein
bo qun c lu hn, cc nt cao su c gn pharaphin v bo qun b gam
mu trong t lnh.
III/ NHN NH V NH GI KT QU:
- Khi tin hnh xc nh Protein trong DNT ta ly 2ml dd DNT cho vo mt ng
nghim thy tinh tng
ng vi nghim ca b gam mu v cho vo 0.2 ml Acid Tricloacetid 30% lc
trn u i chiu vi
g gam mu xc nh c tng ng theo ng mu c nng ng mu suy
ra nng Protein
trong DNT. Trong trng hp c vt qua ng mu 0.8 g/l ta tin hnh pha
long DNT vi nc
mui NaCl 0.9% v tin hnh nh lng nh trn kt qu nhn vi pha long.