Industrial Internship Programme (IIP)

Merck Millipore
Biomanufacturing Sciences and Training Centre
Supervisor: Lau Seat Yee

Muhammad Hilmi Bin Mohamed Zaini

13
th
January 13
th
June 2014
Background of MM Internal TFF Project
Motivation and Objectives
Experimental Set-Up
Methods
Results and Discussion
Conclusions
Reflections
Recent launch of new model of TFF cassettes
from Sartorius
Claims to be on par or better than MM TFF
MM P2 cassettes have been known to perform
better than Sartorius equivalent.
A need to compare MM P3 cassettes against
Sartorius new launch cassettes
Results used to aid sales strategies

Evaluate and compare hydraulic performance
of Brand X TFF Cassettes against that of MMs
P3 cassettes at 8.0, 5.0 and 3.0 LMM feed
fluxes (triplicate/duplicate study)

Produce a flux excursion profile using BSA
solutions of 10, 50 and 100g/L respectively.

Installation
Flushing
Pre-Use NWP
Sanitisation
Flushing
Integrity Test
Minimum Working Volume Determination
Pre-Use NWP
Buffer Equilibration
Initial Hydraulic Performance
TMP Excursion
Protein Recovery
Fouled Membrane Hydraulic Performance
DI Water Flush
Cleaning

Repetition for other 2 BSA concentrations
Integrity Test
Storage with 0.1N NaOH
MM TFF cassettes have
Higher k values
Better reproducibility
Better optimum operating conditions
Met protein retention standards
Better membrane permeability values (cleaned
and fouled)
The functioning of a biomanufacturing
sciences network
Appreciation of management styles
Skills/knowledge/traits required in a working
environment
Application of knowledge gained in university
Influence on career plans