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LABORATORY EVALUATION OF
COAGULATION
I. INTRINSIC & COMMON
A. WHOLE BLOOD
1. LEE AND WHITE
2. SLIDE OR DROP METHOD
3. PLASMA RECALCIFICATION TIME
4. CAPILLARY TUBE METHOD
B. ACTIVATED CLOTTING TIME
C. ACTIVATED PARTIAL
THROMBOPLASTIN TIME

II. EXTRINSIC & COMMON
A. ONE STAGE PROTHROMBIN TIME
B. STYPVEN TIME
C. PROTHROMBIN-PROCONVERTIN TEST

III. INDIRECT TESTS FOR COAGULATION
ABNORMALITIES
A. TWO STAGE PROTHROMBIN TIME
B. PROTHROMBIN CONSUMPTION TIME
C. THROMBOPLASTIN GENERATION
TIME
D. THROMBIN CLOTTING TIME
E. REPTILASE TIME























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F. MODIFIED THROMBIN CLOTTING
TIME

IV. SPECIAL COAGULATION TEST
A. SCREENING TEST
B. TISSUE THROMBOPLASTIN
INHIBITION TEST
C. PLATELET NEUTRALIZATION TEST
D. AGAROSE PLASMA GEL TECHNIQUE
E. BETHESDA INHIBITOR ASSAY
F. COAGULATION FACTOR ASSAYS
1. ONE STAGE QUANTITATIVE
CLOTTING ASSAY
2. RISTOCETIN CO-FACTOR
3. FACTOR VIII IMMUNOLOGIC ASSAY
4. FACTOR XIII ASSAY
5. FLETCHER FACTOR

LABORATORY EVALUATION OF
FIBRINOLYSIS
I. DETERMINATION OF LYSIS TIME
A. WHOLE BLOOD CLOT LYSIS TIME
B. DILUTE WBCLT
C. PLASMA CLOT LYSIS TIME
























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II. EUGLOBULIN LYSIS TIME

III. TEST TO DETERMINE PRESENCE OF
FIBRIN DEGRADATION PRODUCTS
A. PROTAMINE SULFATE DILUTION TEST
B. ETHANOL GELATION TIME
C. LATEX FDP ASSAY
D. LATEX D-DIMER ASSAY
E. TANNED RED CELL
HEMAGGLUTINATION INHIBITION TEST
F. STAPHYLOCOCCAL CLUMPING TEST

LABORATORY EVALUATION FOR
VASCULAR INTEGRITY
I. BLEEDING TIME
II. TOURNIQUET CAPILLARY
RESISTANCE TEST

LABORATORY EVALUATION OF
PLATELET FUNCTION
I. QUANTITATIVE PLATELET TEST
A. PLATELET ESTIMATION
B. ABSOLUTE PLATELET
1. UNOPETTE SYSTEM
2. REESE AND ECKER
C. AUTOMATED























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II. QUALITATIVE PLATELET FUNCTION
TEST
A. PLATELET ADHESION
1. BLEEDING TIME
a. DUKES METHOD
b. IVYS METHOD
c. MODIFIED SIMPLATE
2. GLASS BEAD RETENTION TEST
B. CLOT RETRACTION
1. MCFARLANE
2. HIRSHBOECK METHOD
C. PLATELET FACTOR ASSAYS
1. PF3 AVAILABILITY
2. PF4 AND TG ASSAY
D. PLATELET AGGREGATION TEST