Detection of micrometastases tumor is the main problem in misinterprets tumor staging and

leads to improper therapeutic management. The promoter 2 of Src homology-2-containing

protein-tyrosine phosphatase 1 (SHP1-P2) has been proved for specific
hypermethylatedCpGs in epithelial cell. Here, we used SHP-1P2 methylation to detect the
occult micrometastases of colon cancers in the lymph nodes by performing Combine
Methylation Specific and Taqman Real-Time PCR (COMST Real-Time PCR). We evaluated
Sixty colon cancer patients by selection of selected from histologically positive lymph nodes
(case) were compared with matched histologically negative lymph nodes (control) (*). The
results showed We found that the methylation level in the selected case group was
significantly higher than the control group significantly (95% CI, 24.92 to 38.67; p<0.001).
Moreover, By perforning assessments by the ROC curve analysis, the area under the curve
was 0.8462 (95% CI, 0.7785 to 0.9138; p<0.001). At the cut-off was of 33.42%, sensitivity,
specificity and likelihood ratio were 80.33%, 77.67%, and 3.44 respectively. Consequently,
These mean the SHP-1 P2 methylation level has a potential value to differentiate between
positive and negative lymph nodes and may have benefit in clinical application. Our ongoing
study is examining to examine lymph nodes in the stage I colon cancer patients and the
relationship to recurrence-free survival rate.
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Comments:
1. (*) 60 patients 60 positive lymph nodes paper

Sixty positive lymph nodes from sixty patients were compared with 30 negative lymph nodes
from 26 patients.

2.