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3132 MARGESIN AND SCHINNER APPL. ENVIRON. MICROBIOL.
terfering factors, such as the composition of the contaminant
components and the level of recalcitrant compounds, may in-
uence lipase activity in soils in which the contamination is
aged.
Correlations. Correlations between parameters measured in
the eld study are presented in Table 3. In the fertilized soil, all
biological parameters (microbial counts, soil respiration, en-
zyme activities) correlated signicantly positively with each
other, as well as with the residual hydrocarbon concentration,
indicating the importance of biodegradation. The positive cor-
relations of the available nutrient content with the hydrocar-
bon concentration, the microbial counts, and the activities in
the fertilized soil indicate the relevance of nutrients. The low
microbial activities in the unfertilized soil (Fig. 2D to F), as
well as the lack of correlation with the residual hydrocarbon
concentration, led to the conclusion that a considerable part of
the hydrocarbon loss due to natural attenuation probably had
to be attributed to abiotic processes.
In conclusion, we demonstrated that a signicant reduction
in the level of one of the most common pollutants, diesel oil,
can be achieved even under unfavorable conditions, such as
those present in an alpine glacier area at about 3,000 m above
sea level, an environment in which to our knowledge bioreme-
diation studies have never been performed before. Nonethe-
less, the rate of contaminant removal slowed drastically before
the desired clean-up was complete.
ACKNOWLEDGMENTS
This work was supported by the Land Tyrol and by the Austrian
Federal Ministries of Science and Environment.
We are especially grateful to H. Klier (Wintersport Tirol AG & Co.
Stubaier Bergbahnen KG) for giving us permission to conduct the eld
experiment. We thank M. Neuner (Amt der Tiroler Landesregierung,
Hydrogeographie) for providing the climate data and P. Thurnbichler
for technical assistance.
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