The determination of Fe in an industrial waste stream was carried out by the

o-phenanthroline described in Method 10.1. Using the data shown in the

following table, determine the concentration of Fe in the waste stream.
ppm Fe Absorbance
0.00 0.000
1.00 0.18
!.00 0."#
.00 0.$#"
#.00 0.%!%
unknown 0.!"&
SOLUTION
'inear regression of absorbance (ersus the concentration of Fe in the standards
gi(es
A = 0.000" ) 0.181% * +ppm Fe,
-ubstituting the un.nown/s absorbance into the calibration e*pression gi(es
the concentration of Fe in the waste stream as 1.#8 ppm.