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2009 Biology Examination Paper
2009 Biology Examination Paper
2009 BIOLOGY
FOR OFFICE
USE ONLY
SUPERVISOR
CHECK
QUESTION
BOOKLET
ATTACH SACE REGISTRATION NUMBER LABEL
TO THIS BOX
1
21 pages, 28 questions
RE-MARKED
You will have 10 minutes to read the paper. You must not write in your question booklets, or on your multiplechoice answer sheet, or use a calculator during this reading time but you may make notes on the scribbling
paper provided.
2.
This paper is in three sections: Section A and Part 1 of Section B are in Question Booklet 1; Part 2 of Section B
is in Question Booklet 2; and Section C is in Question Booklet 3.
Section A: Multiple-choice Questions (Questions 1 to 25)
Answer Section A on the separate multiple-choice answer sheet, using black or blue pen.
Answer all questions in Section A.
Section B: Short-answer Questions (Questions 26 to 34)
Answer Part 1 of Section B (Questions 26 to 28) in the spaces provided in Question Booklet 1.
Write on page 21 of Question Booklet 1 if you need more space.
Answer Part 2 of Section B (Questions 29 to 34) in the spaces provided in Question Booklet 2.
Write on page 12 of Question Booklet 2 if you need more space.
Section C: Extended-response Questions (Questions 35 and 36)
Answer both questions in Section C in Question Booklet 3.
Write on page 8 of Question Booklet 3 if you need more space.
3.
In Section B there is no need to ll all the space provided; clear, well-expressed answers are required. If you
delete part or all of an answer you should clearly indicate your nal answer and label it with the appropriate
question number.
4.
5.
Attach your SACE registration number label to the box at the top of this page. Copy the information from
your SACE registration number label into the boxes on your multiple-choice answer sheet and on the front
covers of Question Booklets 2 and 3.
6.
At the end of the examination, place Question Booklets 2 and 3, and your multiple-choice answer sheet, inside
the back cover of Question Booklet 1.
1. Which one of the following macromolecules is not used as an energy reserve in a cell?
J.
Chitin.
K. Lipid.
L. Glycogen.
M. Starch.
2. Which one of the following substances is present in an onion root cell, but not in a human muscle
cell?
J.
DNA.
K. Cellulose.
L. Lipid.
M. Glycogen.
K. the presence of molecules with a shape similar to that of the active site.
L. the presence of molecules with a shape complementary to that of its substrate.
M. increasing the temperature.
4. A retrovirus contains genetic material in the form of RNA. When a retrovirus infects a host cell,
its RNA is used as a template to make DNA, in a process called reverse transcription.
Which one of the following alternatives shows the DNA segment that would be made using the
RNA segment template shown below?
RNA segment template: G C C U A G G
J.
CGGAUCC
K. G C C T A G G
C G GATC C
L. C G G A T C C
M. C G G A U C C
GCCUAGG
5. Refer to the following diagram, which shows process A, compound B, and structures 1 and 2 in a
cell:
aa
aa
structure 1
DNA
aa
process A
aa
compound B
compound B
aa
aa
structure 2
Which one of the following combinations identies process A, compound B, and structures 1
and 2?
Process A
Compound B
Structure 1
Structure 2
J.
transcription
mRNA
ribosome
tRNA
K.
transcription
mRNA
amino acid
anticodon
L.
DNA replication
DNA
ribosome
anticodon
M.
DNA replication
DNA
amino acid
tRNA
6. Refer to the following table, which shows the amino acid sequence for the same part of a
beta-haemoglobin polypeptide in ve species. Each letter represents one amino acid.
Species
ghost bat
rose-ringed
parakeet
hamster
platypus
black-headed
gull
It is reasonable to conclude from the information in the table above that the
J.
K. platypus is more closely related to the ghost bat than to the hamster.
L. rose-ringed parakeet and the black-headed gull have the same DNA sequence.
M. hamster is more closely related to the platypus than to the black-headed gull.
7. Refer to the following diagram, which shows a process involving part of the cell membrane and a
vesicle inside a cell:
outside cell
cell membrane
inside cell
vesicle
Which one of the following statements identies and describes the process illustrated?
J.
8. The following table shows four sets of conditions in which the same number of identical human
skin cells were cultured. All other factors were kept constant.
Which one of the sets of conditions, J, K, L, or M, would result in the highest rate of cell division?
Conditions
Temperature
Light
J.
25C
not present
present
present
K.
35C
present
not present
not present
L.
25C
present
not present
not present
M.
35C
not present
present
present
Carbon dioxide
Oxygen
9. The movement of chloroplasts within the cytoplasm of some green plant cells maximises the
exposure of the chloroplasts to sunlight.
Which one of the following cell structures enables this movement of chloroplasts to occur?
J.
Endoplasmic reticulum.
K. Cytoskeleton.
L. Golgi body.
M. Cell membrane.
10. Refer to the following diagram, which shows an organelle found in a eukaryotic cell:
membrane A
membrane B
The organelle shown above is thought to have evolved by endosymbiotic events approximately
1.5 billion years ago.
Which one of the following combinations identies the organelle, the origin of membrane A, and
the origin of membrane B?
Organelle
Origin of membrane A
Origin of membrane B
J.
mitochondrion
heterotrophic prokaryote
K.
chloroplast
autotrophic prokaryote
L.
chloroplast
autotrophic prokaryote
M.
mitochondrion
heterotrophic prokaryote
11. Two types of gene are involved in regulating the cell cycle. Proto-oncogenes control the start of
cell division, and tumour-suppressor genes stop cell division. A mutated proto-oncogene can result
in cancer. Both proto-oncogenes and tumour-suppressor genes are susceptible to carcinogens.
Which one of the following combinations of type of gene, presence or absence of carcinogen, and
effect on the cell cycle is consistent with the information above?
Type of gene
Carcinogen
J.
proto-oncogene
present
K.
tumour suppressor
present
L.
proto-oncogene
not present
M.
tumour suppressor
not present
12. Refer to the following diagram, which shows two compartments, A and B, separated by a
semi-permeable membrane. The initial volume of solution in each compartment is 5 mL. The
initial concentrations of substances in each compartment are shown in parts per million (ppm).
Compartment A
Compartment B
5 ppm
25 ppm
20 ppm
30 ppm
sucrose
25 ppm
sucrose
35 ppm
semi-permeable membrane
(permeable to K+, Na+, and water, but not to sucrose)
According to the information in the diagram above, there would be an overall movement of
J.
the cell cycle, when mistakes in copying the DNA base sequence occur.
K. the rst division of meiosis, when genes are exchanged between the members of a pair of
homologous chromosomes.
L. the second division of meiosis, when pairs of homologous chromosomes align themselves
independently of each other.
M. fertilisation, when a random combination of gametes forms a zygote.
15. If the body temperature of a healthy person falls below the normal setting of 37C
J.
16. Bacillus thuringiensis (Bt) is a bacterium that synthesises crystal-like proteins (Cry proteins).
Cry proteins bind to specic receptors on the intestinal lining of specic groups of insects,
rupturing the cells and killing the insects within a few days.
Cotton plants do not naturally synthesise Cry proteins. Biotechnology is used to produce cotton
plants that do synthesise Cry proteins.
Which one of the following processes could be used to produce a cotton plant with the potential to
synthesise Cry proteins in all its cells?
J.
Using a bacterial vector to transfer Cry proteins into the cells of a cotton plant.
K. Transferring the genes for Cry proteins into the cells of a cotton plant.
L. Using microinjection to transfer Cry proteins into the fertilised egg cell of a cotton plant.
M. Transferring the genes for Cry proteins into the fertilised egg cell of a cotton plant.
aerobic respiration, autotrophs and heterotrophs convert chemical energy into heat energy.
18. Refer to the following diagram, which shows the respiratory system of an insect:
spiracle
tracheole
trachea
There are many openings called spiracles on the surface of an insects body. Each spiracle
connects to an air tube (trachea) that branches throughout the insects body. Air is moved in and
out of the air tubes by contractions of the insects body.
The many tiny branches (tracheoles) of the air tubes deliver air direct to the body cells of the
insect. There is moisture at the end of each tiny branch. Gas exchange between the tiny branches
and the body cells occurs by diffusion.
Which one of the following descriptions does not apply to both an insect and a human being?
J.
There is a large surface area for gas exchange because of the presence of many tiny
structures.
K. Air is delivered direct from tiny branches to body cells for gas exchange.
L. The mechanism of gas exchange is diffusion.
M. Water is lost by evaporation during gas exchange.
19. Refer to the following diagram, which shows a dividing cell from a diploid organism:
This cell
J.
is undergoing mitosis.
20. Various types of organism, including native bushes, insects, small birds, and lizards, live in the
shrubbery in a suburban backyard.
These organisms together make up
J.
a community.
K. an ecosystem.
L. a population.
M. a habitat.
21. Which one of the following statements describes an example of a mechanism that would help to
maintain reproductive isolation?
J.
K. The production of gametes during the same season by two species of pine tree.
L. The mating and production of infertile offspring by two individuals.
M. The separation of two populations of the same species by a geographical barrier.
10
22. Refer to the following graph, which shows the level of productivity of six types of ecosystem:
10000
8000
6000
4000
2000
0
temperate
forest
savannah
lakes and
streams
type of ecosystem
woodland
agricultural
land
estuaries
Which one of the following statements is most consistent with the data in the graph above?
J.
23. The wounds of patients who have undergone surgery are susceptible to bacterial infections.
Enterococcus faecium and methicillin-resistant Staphylococcus aureus (MRSA) are species of
bacterium associated with these infections. Methicillin and other antibiotics are often used to treat
infections of surgical wounds, but do not always cure them.
Which one of the following statements about the development of resistance to antibiotics in
bacteria such as Enterococcus faecium and MRSA is correct?
J.
K. Bacteria that are exposed to methicillin will become resistant to all antibiotics to which
they are exposed.
L. A number of individuals in a population of bacteria may be resistant to methicillin.
M. A person who is resistant to methicillin will pass on the methicillin-resistant gene to the
bacteria.
11
brown snake
frill-necked lizard
koala
native insect
gum tree
native grass
K. Gum trees and native grasses are both in the rst trophic level.
L. Koalas and desert hopping mice belong to the same trophic level.
M. Brown snakes belong to both the third and the fourth trophic levels.
12
25. Two students investigated anaerobic respiration by taking measurements of the volume of carbon
dioxide (CO2) produced by yeast during bread-making.
Both students used the same experimental conditions when collecting the data recorded in the
table below:
Sample number
Average
Student B
1.02
1.01
1.10
1.02
1.15
1.02
0.99
1.03
1.09
1.02
1.07
1.02
If the true value for this experiment was 1.05 (arbitrary units), the data in the table indicate that
the set of data collected by student A
J.
has the same random errors as the set of data collected by student B.
K. has random errors but the set of data collected by student B does not.
L. is more accurate than the set of data collected by student B.
M. is more reliable than the set of data collected by student B.
13
26. Silk, which is produced by spiders when building webs, is one of the strongest substances known.
It is ve times stronger than steel in a weight-to-weight comparison, more elastic than nylon, and
lighter and stronger than Kevlar. Its many potential uses include body armour in the military,
articial tendons, medical sutures, and biodegradable shing lines.
Spider silk is made of a protein. Scientists have located and isolated the gene for the spider silk
protein from orb-weaving spiders that produce the strongest silk.
(a) Describe the features of a probe that would be used to locate the gene for the spider silk
protein in the spider DNA.
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marks)
(b) Scientists hope to use genetic engineering techniques to create synthetic spider silk that
can be used commercially. Their rst attempts have involved genetically modifying
bacterial cells to produce the spider silk protein.
Describe the process that the scientists would use to insert the isolated gene for the spider silk
protein into the plasmid of a bacterial cell.
_______________________________________________________________________________________________________
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14
marks)
(c) Two amino acids, glycine and alanine, make up 70% of spider silk protein. Bacterial
cells that were genetically modied to produce spider silk protein died as a result of the
depletion of these two amino acids.
Explain why the bacterial cells died as a result of the depletion of these two amino acids.
_______________________________________________________________________________________________________
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marks)
(d) Scientists have recently inserted the gene for the spider silk protein into goats in such a
way that it is present in all the goats cells, but is expressed only in their milk-producing
cells. These spider-goats produce between 1 and 2 grams of spider silk in every litre of
milk.
Explain one argument that may be used against the genetic modication of goats to produce
silk.
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15
marks)
27. Refer to the following diagram, which shows the movement of acetylcholine, a chemical that
moves across the synapse (the gap between two nerve cells):
nerve cell 2
acetylcholine
nerve cell 1
vesicle
synapse
[This diagram is not drawn to scale.]
(a) Describe the process by which nerve cell 1 releases acetylcholine into the synapse, as
shown in the diagram above.
_______________________________________________________________________________________________________
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marks)
(b) Name the organelle that forms the vesicles containing acetylcholine.
____________________________________________________________________________________________ (2
marks)
(c) After acetylcholine has been released into the synapse, it binds to specic membrane
receptors on nerve cell 2 and triggers a nerve impulse.
Explain why acetylcholine will bind only to acetylcholine membrane receptors and not to
membrane receptors for any other chemical.
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16
marks)
(d) Acetylcholine must be broken down very quickly after it has triggered a nerve impulse,
in order to reset the synapse in preparation for the next nerve impulse. The enzyme
acetylcholinesterase (AChE) breaks acetylcholine down into choline and acetate at the rate
of 5000 molecules per second per molecule of enzyme. The breakdown of acetylcholine
is represented by the following word equation:
acetylcholine
AChE
choline + acetate
Explain how AChE lowers the activation energy needed for this reaction.
_______________________________________________________________________________________________________
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marks)
(e) Diisopropyl uorophosphate (DIFP) is a nerve toxin that inhibits AChE. The subsequent
accumulation of acetylcholine in the synapse results in paralysis of muscles, including the
respiratory muscles, followed by death.
Explain how the presence of DIFP results in an accumulation of acetylcholine in the synapse.
_______________________________________________________________________________________________________
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17
marks)
28. Refer to the following diagram, which shows a pair of guard cells. Guard cells are specialised
photosynthetic cells found on the leaf surfaces of many green plants.
chloroplast
guard cell
guard cell
vacuole
epidermal cell
(midnight)
3000
12
16
20
(noon)
4000
4750
5000
24
(midnight)
4750
4000
3000
(a) On the grid below, graph the data in the table above to show the change in volume of
the guard cell over the 24-hour period.
(4 marks)
18
(b) Describe the change in volume of the guard cell over the 24-hour period.
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marks)
(c) Using only the information provided, state one factor in the design of this experiment
that would affect the reliability of the results.
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marks)
marks)
(e) It was later found that a systematic error had affected the results of the experiment.
Explain how this systematic error would have been identied.
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marks)
(f) Explain how an increase in its volume may affect a guard cells ability to obtain the
materials it needs from its environment.
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19
marks)
(g) Potassium ions (K+) can move against a concentration gradient through the cell membrane
and into the guard cells.
The diagram below shows a cross section of the cell membrane from a guard cell:
outside
guard cell
potassium ions
X
X
ATP
inside
guard cell
ADP
(i) Name the cellular process by which potassium ions move into the guard cell against
a concentration gradient.
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marks)
marks)
(iii) Explain how the process of aerobic respiration enables ATP to be formed from ADP.
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20
marks)
You may write on this page if you need more space to nish your answers to any questions in
Part 1 of Section B. Make sure to label each answer carefully (e.g. 27(a) continued).
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21
2009 BIOLOGY
FOR OFFICE
USE ONLY
SUPERVISOR
CHECK
QUESTION
BOOKLET
CHECK
LETTER
SEQ
BIN
BIOLOGY
RE-MARKED
Part 2 of Section B
2
12 pages, 6 questions
29. Very small amounts of DNA can be collected from a crime scene.
(a) State how the polymerase chain reaction (PCR) enables very small amounts of DNA from
a crime scene to be used as evidence.
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marks)
(b) Refer to the following diagram, which shows three DNA ngerprints: a mixed sample of
DNA from a crime scene, the DNA of the victim, and the DNA of one suspect:
DNA
fragment
Mixed sample
of DNA from
crime scene
DNA of
victim
DNA of one
suspect
A
B
C
D
E
F
With reference to the information in the diagram above, explain how the DNA evidence could
be used to support the suspects innocence.
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marks)
30. The human eye is a sensory organ. Refer to the following diagram, which shows the location of
the iris, cornea, retina, and optic nerve in the human eye:
retina
iris
optic nerve
cornea
marks)
(b) Receptor cells in the retina transmit information, via the optic nerve, to the brain. Cells
in the cornea have receptors for steroid hormones.
Describe two differences between the transmission of information by nerves and the
transmission of information by hormones.
Difference 1: ________________________________________________________________________________________
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Difference 2: ________________________________________________________________________________________
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marks)
(c) Receptor cells in the retina transmit information, via nerves, to the dilator muscle in the
iris. The dilator muscle alters the size of the iris and consequently changes the amount of
light entering the eye. This is a reex response.
(i) Name the effector in the reex response described above.
_______________________________________________________________________________________ (2
marks)
marks)
31. It is necessary for double-stranded DNA to break for the process of crossing over to occur.
In an experiment to investigate the number and position of double-stranded DNA breaks (DSBs),
biologists examined a section of DNA from a species of yeast.
The graph below shows the results of this experiment:
number of DSBs
30
25
1 kbp
20
15
10
5
0
gene 1 gene 2
gene 3
gene 4
gene 5 gene 6
Genes 1, 3, 5, and 6 code for proteins. Genes 2 and 4 produce non-protein coding RNA (ncRNA).
(a) Using the scale in the graph above, estimate the length in kilobase pairs (kbp) of gene 4.
____________________________________________________________________________________________ (2
marks)
marks)
(ii) name the gene in which the greatest number of DSBs occurs.
_______________________________________________________________________________________ (2
marks)
(c) The biologists who obtained the results shown in the graph above were investigating the
connection between sections of DNA that produce ncRNA and the likelihood of DSBs
occurring.
State one conclusion that is consistent with these results.
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marks)
(d) (i) In Box 1, draw a diagram to show one occurrence of crossing over in one pair of
chromosomes.
Box 1
(2 marks)
(ii) In Box 2, draw a diagram to show the result of the crossing over that you have
drawn in Box 1.
Box 2
(2 marks)
(e) Explain what would be the likely consequence for a cell if DSBs occurred in protein
coding sections of DNA.
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marks)
32. Smoking increases the risk of developing lung cancer more in some people than in others.
Scientists say that a simple urine test can predict whether or not a smoker is likely to develop lung
cancer.
NNAL is a product of a carcinogen in cigarette smoke. Experimental trials found that the
likelihood of developing lung cancer is 8.5 times greater for smokers with the highest levels of
NNAL in their urine than for those with the lowest levels.
The following diagram shows part of a human nephron:
direction of blood flow
(a) On the diagram above, draw an arrow to show the pathway followed by a molecule, such
as NNAL, as it travels from the blood to the urine.
(2 marks)
(b) Describe one way in which the ndings of the experimental trials involving NNAL and
smokers may benet society.
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marks)
33. Refer to the following photograph, which shows a young zenkey. A zenkey is the offspring
produced from the mating of a male zebra (with a diploid number of 44) and a female donkey
(with a diploid number of 62).
Source: www.smh.com.au
marks)
(b) The cells of zenkeys are able to undergo mitotic cell division.
Describe two consecutive steps that involve the movement of chromosomes during mitotic
cell division.
Step 1:_______________________________________________________________________________________________
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Step 2:_______________________________________________________________________________________________
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marks)
(c) State one factor that would prevent the cells of a zenkey from completing meiosis.
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marks)
34. In the wet season on the African plains, animals such as wildebeest migrate to follow the
availability of grasses containing high levels of phosphates and nitrates. The wildebeest eat the
leaves, but not the stems, of the grasses.
Acacia is a plant that threatens the growth of the grasses. Acacia seeds are widely distributed
during the dry season, and germinate in the wet season. As the grasses are eaten by the wildebeest,
the acacias have more resources available to them and grow into large trees. The conditions are
then no longer suitable for the grasses, and the wildebeest move away to nd food elsewhere.
Acacias are a source of food for animals such as the dik-dik, a type of antelope. Dik-diks eat all
the easily accessible leaves of the acacias.
Towards the end of the wet season the acacias are destroyed by elephants, which knock them
down and eat the branches.
marks)
(b) Name the process described above that results in a change in the mixture of species.
____________________________________________________________________________________________ (2
marks)
marks)
(ii) explain how the destruction of the acacias enables the population of grasses to
increase at the beginning of the next wet season.
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10
marks)
11
marks)
You may write on this page if you need more space to nish your answers to any questions in
Part 2 of Section B. Make sure to label each answer carefully (e.g. 33(b) continued).
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12
2009 BIOLOGY
FOR OFFICE
USE ONLY
SUPERVISOR
CHECK
QUESTION
BOOKLET
CHECK
LETTER
SEQ
BIN
BIOLOGY
RE-MARKED
Section C
3
8 pages, 2 questions
35. The efcient supply of oxygen and glucose to cells enhances athletic performance. At times the
supply of oxygen to cells is inadequate to meet their energy demand. Some athletes choose to use
performance-enhancing drugs to improve the supply of oxygen and / or glucose to their cells.
Explain how the structure of blood capillaries facilitates the supply of glucose to cells.
Describe how energy is made available in the cells of athletes under anaerobic conditions.
Describe how choices about drug use can affect the well-being of an athlete.
(15 marks)
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36. The human population is approaching 7 billion. If growth trends continue, the human population
will reach 9 billion by 2050.
Use the human species as an example to explain the idea that r and K strategies are
extremes on a continuum.
Explain why the exponential growth of the human population is not sustainable.
Discuss one ethical issue associated with the level of human population predicted for 2050.
(15 marks)
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You may write on this page if you need more space to nish your answers to Questions 35 and 36.
Make sure to label each answer carefully (e.g. 35 continued).
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BIOLOGY 2009
ACKNOWLEDGMENT
The SACE Board of South Australia has made every effort to trace copyright holders. If however,
any material has been incorrectly acknowledged, we apologise and invite the copyright holder to
contact us.