PROBLEM 1.

V, mol/(L-min)
S, mol/L
no inhibidor inhibidor
3.30E-04
5.60E+07
3.70E+07
5.00E-04
7.10E+07
4.70E+07
6.70E-04
8.80E+07
6.10E+07
1.65E-03
1.29E+08
1.03E+08
2.21E-03
1.49E+08
1.25E+08
1/S
1/V
1/V
3.03E+03
1.79E-08
2.70E-08
2.00E+03
1.41E-08
2.13E-08
1.49E+03
1.14E-08
1.64E-08
6.06E+02
7.75E-09
9.71E-09
4.52E+02
6.71E-09
8.00E-09

no inhibidor
y= 4E-12x + 5E-09
4E-12x + 5E-09=0
x= 5E-09 / 4E-12
x=
1250
km= 1 / 1250
Km=
0.0008
m= Km / Vmax
4E-12 = 0.0008 / Vmax
Vmax= 0.0008 / 4E-12
Vmax= 200000000

inhibidor
y = 7E-12x + 5E-09
7E-12x + 5E-09=0
x=5E-09 / 7E-12
714.285714
Km=1 / 714.285
0.0014
m= Km / Vmax
7E-12= 0.0014 / Vmax
Vmax= 0.0014 / 7E-12
200000000

Ki= ((I)*((Km/Vmax)*(1/S)))/((1/V)-(1/Vmax))
Ki=
0.000105581

COMPETITIVO

3.00E-08
2.50E-08

y = 7E-12x + 5E-09
R = 0.991

2.00E-08
NO
1.50E-08
1.00E-08

CON
y = 4E-12x + 5E-09
R = 0.996

5.00E-09
0.00E+00
0.00E+005.00E+021.00E+031.50E+032.00E+032.50E+033.00E+033.50E+03

Linear (NO)
Linear (CON)

PROBLEMA 3.
V
0.909
0.658
0.493
0.4
0.333
0.298
0.227

S
20
10
6.67
5
4
3.33
2.5

1/S
0.05
0.1
0.149925037
0.2
0.25
0.3003003
0.4

1/V
1.10011
1.5197568
2.0283976
2.5
3.003003
3.3557047
4.4052863

km= 9.2
y = 9.4109x + 0.6092
9.4109x + 0.6092=0
x= 0.064733447
Km= 1/ 0.064
Km= 15.44796454
m= Km / Vmax
9.4109 = 15.4479 / Vmax
Vmax= 15.4479 / 9.4109
Vmax= 1.641497045

COMPETITIVO

Ki= ((I)*((Km/Vmax)*(1/S)))/((1/V)-(1/Vmax))
Ki= 0.991642355

5
4.5
4

y = 9.4109x + 0.6092
R = 0.9986

3.5
3

Series1

2.5

Linear (Series1)

2
1.5
1
0.5
0
0

0.1

0.2

0.3

0.4

0.5

PROBLEMA 4.
E0 (g/l)
1.6
1.6
1.6
1.6
1.6
1.6
1.6
1.6
1.6
1.6
0.92
0.92
0.92
0.92
0.92
0.92

T(C)
30
30
30
30
30
49.6
49.6
49.6
49.6
49.6
30
30
30
30
30
30

l(mmol/ml)
0
0
0
0
0
0
0
0
0
0
0
0
0
0.6
0.6
0.6

S (mmol/ml)
0.1
0.033
0.02
0.01
0.005
0.1
0.033
0.01
0.0067
0.005
0.1
0.02
0.01
0.1
0.033
0.02

30C
y = 0.0074x + 0.3035
0.0074 x+ 0.3035 = 0
x=0.3035 / 0.0074
x=
41.01351351
km=1 / 41.0135
km= 0.024382208
m= Km / Vmax
0.0074 = 0.02438/Vmax
Vmax= 0.02438 / 0.0074
Vmax = 3.294892916

V(mmolml-min)
2.63
1.92
1.47
0.96
0.56
5.13
3.7
1.89
1.43
1.11
1.64
0.9
0.58
1.33
0.8
0.57

40C
y = 0.0037x + 0.1577
0.0037x + 0.1577=0
x=0.1577 / 0.0037
42.62162162
km=1 / 42.6216
0.02346227
m= Km / Vmax
0.0037 = 0.02346 / Vmax
Vmax= 0.02346 / 0.0037
6.34115409

ACOMPETITIVO
k1
Ki= (I)/((1/v)-(((km/Vmax)*(1/S))*(Vmax-1)))
1.19094883
no competitivo

1/S
10
30.3030303
50
100
200
10
30.3030303
100
149.2537313
200
10
50
100
10
30.3030303
50

1/V
0.38022814
0.52083333
0.68027211
1.04166667
1.78571429
0.19493177
0.27027027
0.52910053
0.6993007
0.9009009
0.6097561
1.11111111
1.72413793
0.7518797
1.25
1.75438596

Inhibidor
y = 0.0251x + 0.4978
0.0251x + 0.4978=0
x=0.4978 / 0.0251
19.832669
Km= 1 / 19.8326
0.0504219
m= Km / Vmax
0.0251 = 0.5042 / Vmax
Vmax = 0.05042 / 0.0251
2.0088389

2
1.8

y = 0.0251x + 0.4978
R = 0.9998

1.6

y = 0.0074x + 0.3035
R = 0.9999

1.4
1.2

40C

30C

0.8

inhibidor

y = 0.0037x + 0.1577
R = 0.9997

0.6

Linear (40C)
Linear (30C)

0.4

Linear (inhibidor)

0.2
0
0

50

100

150

200

250