CONFIDENTIAL, ASIJUN 2014/B10462 UNIVERSITI TEKNOLOGI! MARA FINAL EXAMINATION COURSE : BIOCHEMISTRY COURSE CODE BlO462 EXAMINATION JUNE 2014 TIME 2 HOURS INSTRUCTIONS TO CANDIDATES 1 This question paper consists of seven (7) questions. 2. Answer ALL questions in the Answer Booklet. Start each answer on a new page. 3. Do not bring any material into the examination room unless permission is given by the invigilator. 4 Please check to make sure that this examination pack consists of: i) the Question Paper ii) an Answer Booklet - provided by the Faculty DO NOT TURN THIS PAGE UNTIL YOU ARE TOLD TO DO SO This examination paper consists of printed pages © Hak Cipta Universiti Teknologi MARA CONFIDENTIALCONFIDENTIAL 2 ASIJUN 2014/B10462 QUESTION 1 a) Relate the importance of biochemistry to an individual. (2 marks) b) Explain why water has such an interesting and unique properties. (3 marks) ©) Membrane protein is divided into two classes. Name the class of proteins which can be easily dissociated with mild detergent treatment. (1 mark) 4) i) Name one yellow component that can enhance the order and rigidity of a plasma membrane. (1 mark) ji) Explain the structure and interactions involved in Question 4 (d (i). (2marks) €) State the passive transport which can transport small, uncharged molecules such as Oz, Ne and CO, through a membrane. (1 mark) QUESTION 2 a) List three main roles of carbohydrates, (3 marks) b) Consider the aldopentoses in Figure 1 Hip Ho Ho Ho teton nou Hot Hey-o# Hon HoH HoH OH HoH HoH HoH sCHeoH Heo Cheon CHeoH A B c D Figure 4 i) Name the type of stereoisomers represented by B and C. (1 mark) ii) Name the type of stereoisomers represented by C and D. (1: mark) iil) Name sugar A. (1 mark) (© Hak Cipta Universiti Teknologi MARA, CONFIDENTIAL,CONFIDENTIAL 3 ASIJUN 20141810462 iv) Illustrate the c~anomeric form of the furanose Haworth ring structure for sugar A. (1 mark) ©) i) Sketch the structures of sucrose, maltose and lactose. (3 marks) ii) Give one natural source for one common disaccharide. (1 mark) QUESTION 3 a) Name the structure shown in Figure 2. (1: mark) H.C —CH—CH, 71 ON 9 9 6 1 1 1 o=¢ ¢=0 c=0 Figure 2 b) State the lipid group in which the structure in Figure 2 belongs to. (1 mark) c) List three advantages of the lipid group in Question 3 (b). (3 marks) ) Briefly explain the connection between acetone and acetyl-coA in lipid metabolism. (2 marks) €) Give one ketone body besides acetone. (1 mark) f) Briefly explain the fate of the end product for an odd number of carbons in B-oxidation (2 marks) (© Hak Cipta Universiti Teknologi MARA CONFIDENTIALCONFIDENTIAL 4 ASIJUN 2014/B10462 QUESTION 4 @) i) Name the joining bond between the ribonucleoside components of RNA. (1 mark) li) Name base pairs that are available in RNA. (1 mark) lil) Name the three major classes of RNA in a cell. (3 marks) b) The template strand of a segment of the double-helical DNA contains the following sequence : 5'-CTTAACACCCCTGACTICGCGCCGTCG-3' ) Interpret the base sequence of the coding DNA strand. (1 mark) ji) Express the base sequence of the mRNA that can be transcribed from the template strand starting from the Send, (2 marks) ii) Explain whether the coding ONA strand can be transcribed and translated. (3 marks) ©) Explain the termination process of translation. (3 marks) QUESTION 5 8) Describe the formation of an enzyme-substrate complex. (marks) b) Give five examples of polar amino acids. (6 marks) ©) Explain secondary and tertiary protein structures. (4 marks) @) i) Relate protein abnormalities to diseases. (3 marks) ii) Give three examples of related diseases. (3 marks) (© Hak Cipta Universiti Teknologi MARA CONFIDENTIALCONFIDENTIAL, 5 ASIJUN 2014/B10462 e) Compare the two situations below based on the following: name of the analytical method, principle of the analytical method, form of sample and type of result. Situation 1: Samad is trying to analyze a mixture of proteins from winged bean (Psophocarpus tetragonolobus) seeds with respect to molecular mass. Situation 2: Akmal has been asked by his project supervisor to study the 3D structure of bacterial protease enzyme (10 marks) QUESTION 6 a) Illustrate and explain the Tricarboxylic Acid Cycle (TCA cycle). (10 marks) b) Explain on the fact that when O, does not participate directly in the reactions of the TCA cycle, the cycle operates only under aerobic conditions. (2 marks) ©) Indicate the three TCA cycle intermediates that may be used as biosynthetic precursors, (3 marks) QUESTION 7 Illustrate and explain the biosynthesis of palmitate. (10 marks) END OF QUESTION PAPER (© Hak Cipta Universiti Teknologi MARA. CONFIDENTIAL