45

4000

3435.33

Transmltance (%)
70

65

60
1629.97
1571.74
1417.20

80

75

3500

3000

2500

Wave numbers (cmG 1)

2000

1500

1000
657.43
612.43
634.85

1151.87
1115.10
1048.37

2359.97

100

95

90

85

55

50

40

500

3.77077
X1 = A: Temperature (C)
X2 = C: Time (H)
Actual factors
B:Agitation (rpm) = 100.00
D: Inoculum (%) = 10.00

50
40
Ethanol (g L G 1)

Design-Expert Software
Factor Coding: Actual Bthanol (g L G 1)
BDesign point above predicted value
B Design point below predicted value
Std Error Shading
33.9369

30
20
10
0
92.00
45.00
84.00
42.00
76.00
68.00
39.00
60.00
52.00
33.00 A: Temperature (C)
C: Time (h)
44.00
36.00 30.00

3.77077
X1 = A: Temperature (C)
X2 = C: Time (H)

Design-Expert Software
Factor Coding: Actual Bthanol (g L G 1)
BDesign point above predicted value
B Design point below predicted value
Std Error Shading
33.9369
3.77077
X1 = A: Temperature (C)
X2 = C: Time (H)
Actual factors
A: Temperature = 37.50
C: Time (H) = 64.00

20
10

5.00 30.00

45.00
42.00
39.00
33.00 A: Temperature (C)

50
40
30
20
10
0
92.0084.00
76.0068.00
60.00
75.00
C: Time (h) 52.0044.00
36.00 50.00

160.00
125.00
100.00
B: Agitation (rpm)

50
40
Ethanol (g L G 1)

Actual factors
A: Temperature (C) = 37.80
D: Inoculum (%) = 10.00

30

11.00
D: Inoculum (%) 9.00 7.00

Ethanol (g L G 1)

3.77077
X1 = A: Temperature (C)
X2 = C: Time (H)

40

0
15.00
13.00

Actual factors
B:Agitation (rpm) = 100.00
D: Inoculum (%) = 10.00

Design-Expert Software
Factor Coding: Actual Bthanol (g L G 1)
BDesign point above predicted value
B Design point below predicted value
Std Error Shading
33.9369

50
Ethanol (g L G 1)

Design-Expert Software
Factor Coding: Actual Bthanol (g L G 1)
BDesign point above predicted value
B Design point below predicted value
Std Error Shading
33.9369

30
20
10
0
15.00
13.00
11.00
9.00
D: Inoculum (%)
7.00

160.00
125.00
5.00 50.00

100.00
75.00 B: Agitation (rpm)