Procedure

Dissolved Oxygen and Kla

First the medium in the reactor was purged with nitrogen
to remove any oxygen from the medium. Then set the stir
speed to 200 rpm and the air flow was turned on and set
to 2 L/min. The dissolved oxygen content was recorded
every thirty seconds until steady state was reached. The
system was purged with nitrogen again. The stir speed
reset and the process repeated at 300, 400 and 500 rpm
respectively at 2 L/min and 200,300, 400, and 500 rpm at
4 L/min.

Fermentation
To start the fermentation, 100mL of E Coli culture (grown
overnight) and glucose solution were added to a second
reactor. The stir speed was set to 200 rpm with an oxygen

flowrate of 4L/min. At 30 minute intervals, 5mL samples

were collected and their optical densities were measured
using spectrometer at 600nm to determine the e coli
concentration. To determine the glucose concentration,
0.250 ml of sample were added to 0.75mL of distilled
water and 2 mL DNS, the glucose indicator. The mixture
was in a hot water bath and set at 90 degree Celsius. The
sample was left in the water bath for exactly 5 minutes.
Once removed from the water bath, 2mL of water was
added to the sample and the sample was put in a room
water bath for approximately 2 minutes in other to allow
the sample to reach room temperature. A few ml of the
sample was put into the spectrometer at 570nm. The
data was collected until biomass cell growth reached
stationary phase.