BORDETELLA PERTUSIS

Kingdom: Eubacterium (eukaryotic bacteria)

Phylum : Coccobacillus
Class
: bacillus (rod shaped)
Order
: coccobacillus
Genus
: Bordetella
Species : B.pertussis
B. Pertusis
Penyebab whooping cough / batuk rejan
infeksi sal. Nafas akut, batuk spasmodik slm
fase paroxysmal
B. Parapertusis lebih ringan
B. Bronchiseptica Inf. sal. nafas pd man & hwn

MORFOLOGI
- cocobacilli, kecil, 0,2-0,3 X 0,5-1 m ,
ssn singel/ berpasangan /
cluster kecil
- Gram (-)
- kapsul hny pd strain
tertentu
- spora (-)

Kultur :
- media Bordet Gengou (potato glycerol blood)
- aerob
- hemolisis +
- koloni : kecil, smooth, convex, pearl-like

Struktur Ag
- pili : perlekatan pd epitel mukosa nafas atas
filamentous hemagglutinin
- toksin pertusis
- Lipopolisakarida dinding sel kerusakan epitel
sal nafas bag atas

Toksin pertusis
-Protein exotoxin disekresi invivo & in vitro
- Sub unit :
S1 sub unit A aktivitas biologik
S2-S4 sub unit B - perlekatan
S3-S5
- aktivitas
hemaglutinin
- Hny pd
B. pertusis

Heat labil toxin

Proteinacenous dermonecrotic BM 100.000
pd protoplasma efek vasokonstriksi kuat pd
fase awal pertusis
Adenylat Cyclase toxin
Penetrasi pd sel host CAMP hambat respon
Fagosit oksidatif & sel NK
Tracheal cytotoxin
Scr kimiawi related dgn peptidoglikan merusak
Sel silia pd hamster
Lipopolisakarida
Heat stable LOS endotoxin Gram (-)
Agglutinogen (AGG 1-14)
Ag permukaan aglutinasi Ab
AGG1- B.pertusis LOS , AGG14 parapertusis-pili

MANIFESTASI KLINIS
- Inkubasi 1-2 mgg
- catharral phase :
demam, rhinorrhea,
batuk progresif
1-2 mgg
- Paroxysmal phase
2-4 mgg :
batuk berat,
spasmodik,
lekositosis ( > 100.000/mm3)

- Convalescense phase
1-3 mgg, penurunan batuk -- normal
Komplikasi bronchopneumonia, encelophaty akut
Konvulsi kerusakan otak

PATOGENESIS
- Transmisi via droplet
- Kolonisasi multiplikasi cpt pd mukosa sal. Nafas
- (-) bakteremia

Imunitas

Relationship of B pertussis to the developing antibody response during whooping cough.

DX/ LAB
- specimen: swab nasofaring, cucian rongga hidung
- uji antibodi fluoresen langsung
- kultur
TERAPI
Tetracyclin, erythromycin, Chloramphenicol
Preventif vaksinasi DPT