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LessonPlanTitle

GelElectrophoresisLab

Grade

10

Subject

Biology

Standard(s)

http://www.nextgenscience.org/

HSPS13:Planandconductaninvestigationtogatherevidence

tocompare
thestructureofsubstancesatthebulkscale

toinferthestrengthofelectrical
forcesbetweenparticles.

HSLS12:Developanduseamodeltoillustratethehierarchicalorganization
ofinteractingsystemsthatprovidespecificfunctionswithinmulticellular
organisms.

Literacy
CCSS.ELALITERACY.RST.910.3
Followpreciselyacomplexmultistepprocedurewhencarryingout
experiments,takingmeasurements,orperformingtechnicaltasks,attending
tospecialcasesorexceptionsdefinedinthetext.

Time

45mins(Note:Completethislabduringadoubleperioddayto
makesureallofthestudentshaveenoughtimetofinishthe
experimentandstarttheconclusionquestions.

Objective(s)

Todeterminewhethergelelectrophoresisisanefficientseparation
technique.Applycriticalliteracyskillstothetextandsupplemental
literatureusedtocompletethislab.

Materials/Technology gelagarose/energysource
Requiredforthe
electrophoresistray/papertowels
lesson
pipettes/smallcontainer
dye/cameraorrecordingdevice
seelinkfordetails:
http://learn.genetics.utah.edu/content/labs/gel/
Initiation

Goovertheprelab.Havetheclasscollectivelyagreeonthe
purposeoftheexperiment.

LearningProcedures

1. DI
2. SIOPmodel(scientificlanguage)
3. Talkaboutlabsafety

Grouping

labpairs,wholegroup
Intervention
:Givethestudentsanextranighttoclarifyanswers
totheconclusionquestionsandrepairtheirprelaberrors
beforegradingthisassignment.
Enrichment
:Haveonestudentvideotapeortakepicturesofthe

demonstration.Makeadigitalstoryoryoutubevideoand
postitontheclasswebsite.Assignasupplementalreading
(scientificresearcharticle)thatincludestheapplicationof
thetechniqueusedinthislab.

GuidedPractice

DIandthinkaloud

Instructional
Strategies

DI,integrationofnewliteracies,andGuidedImagery

Closure

Haveastudentvideotapetheteachercompletingthelab
demonstration.

IndependentPractice

Studentsshouldwatchtheflippedclassroomandemailorvisit
theteacheriftheyhaveanyquestionswhilecompletingtheir
labreport.

TeachersNotes

DoaGuidedImageryactivitytomorrowtoactivatethe
studentspriorknowledge.Thisisnotacomplicatedlab,but
certainthingscouldgowrongtoincreasethepercenterror
oftheexperiment.Remindthestudentsofthepossible
causesoferrorbeforethelab.

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