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GelElectrophoresisLab
Grade
10
Subject
Biology
Standard(s)
http://www.nextgenscience.org/
HSPS13:Planandconductaninvestigationtogatherevidence
tocompare
thestructureofsubstancesatthebulkscale
toinferthestrengthofelectrical
forcesbetweenparticles.
HSLS12:Developanduseamodeltoillustratethehierarchicalorganization
ofinteractingsystemsthatprovidespecificfunctionswithinmulticellular
organisms.
Literacy
CCSS.ELALITERACY.RST.910.3
Followpreciselyacomplexmultistepprocedurewhencarryingout
experiments,takingmeasurements,orperformingtechnicaltasks,attending
tospecialcasesorexceptionsdefinedinthetext.
Time
45mins(Note:Completethislabduringadoubleperioddayto
makesureallofthestudentshaveenoughtimetofinishthe
experimentandstarttheconclusionquestions.
Objective(s)
Todeterminewhethergelelectrophoresisisanefficientseparation
technique.Applycriticalliteracyskillstothetextandsupplemental
literatureusedtocompletethislab.
Materials/Technology gelagarose/energysource
Requiredforthe
electrophoresistray/papertowels
lesson
pipettes/smallcontainer
dye/cameraorrecordingdevice
seelinkfordetails:
http://learn.genetics.utah.edu/content/labs/gel/
Initiation
Goovertheprelab.Havetheclasscollectivelyagreeonthe
purposeoftheexperiment.
LearningProcedures
1. DI
2. SIOPmodel(scientificlanguage)
3. Talkaboutlabsafety
Grouping
labpairs,wholegroup
Intervention
:Givethestudentsanextranighttoclarifyanswers
totheconclusionquestionsandrepairtheirprelaberrors
beforegradingthisassignment.
Enrichment
:Haveonestudentvideotapeortakepicturesofthe
demonstration.Makeadigitalstoryoryoutubevideoand
postitontheclasswebsite.Assignasupplementalreading
(scientificresearcharticle)thatincludestheapplicationof
thetechniqueusedinthislab.
GuidedPractice
DIandthinkaloud
Instructional
Strategies
DI,integrationofnewliteracies,andGuidedImagery
Closure
Haveastudentvideotapetheteachercompletingthelab
demonstration.
IndependentPractice
Studentsshouldwatchtheflippedclassroomandemailorvisit
theteacheriftheyhaveanyquestionswhilecompletingtheir
labreport.
TeachersNotes
DoaGuidedImageryactivitytomorrowtoactivatethe
studentspriorknowledge.Thisisnotacomplicatedlab,but
certainthingscouldgowrongtoincreasethepercenterror
oftheexperiment.Remindthestudentsofthepossible
causesoferrorbeforethelab.