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Amersham AKTA Explorer 100 FPLC User Guide Harlowscientific
Amersham AKTA Explorer 100 FPLC User Guide Harlowscientific
Explorer 100 / 10
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amersham bioscience
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bios
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ence
KTAexplorer
KTAexplorer
Injector
Buffers
Box
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biosc
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amers
Column
Valves
Monitor
Conductivity
pH
ham
amers
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bio scie
Outlet
Valve
UV Detector
3 Wavelengths
amersham bioscience
Pumps
am
ersh
am
bios
ci
ence
KTAexplorer
Pump A
pH/cell
electrode
Buffer switching
valve
Sample
Valve
Sample
holder
KTAexplorer 100
In-line
filter
Injection
valve (V1)
1 7
6
2
3 45
Mixer
System
pumps
Column
selection
valve (V2)
UV
3 Wavelength
detector
Cond
Conductivity
monitor
pH
1 87
2
6
345
Sample
pump
1 87
2
6
345
1 7
6
2
3 45
Reverse
flow valve (V7)
pH detector
Fraction
collector
Frac-950
1 87
2
6
345
1 87
2
6
345
Buffer
select
valve (V6)
Outlet
valve (V4)
1 87
2
6
345
Sample selection
valve (V5)
Column
selection
valve (V3)
Mixer
System
pumps
Reverse
flow valve (V7)
Column
selection
valve (V2)
UV
3 Wavelength
detector
Cond
Conductivity
monitor
pH
1 87
2
6
345
Air
sensor
Sample
pump
1 87
2
6
345
1 7
6
2
3 45
pH detector
Fraction
collector
Frac-950
1 87
2
6
345
1 87
2
6
345
Buffer
select
valve (V6)
1 87
2
6
345
Sample selection
valve (V5)
Column
selection
valve (V3)
Outlet
valve (V4)
KTAexplorer 10
In-line
filter
Injection
valve (V1)
1 7
6
2
3 45
Mixer
System
pumps
Column
selection
valve (V2)
UV
3 Wavelength
detector
Cond
Conductivity
monitor
pH
1 87
2
6
345
pH detector
Fraction
collector
Frac-950
1 87
2
6
345
1 87
2
6
345
Buffer
select
valve (V6)
Outlet
valve (V4)
1 87
2
6
345
Column
selection
valve (V3)
KTAexplorer 10 S
In-line
filter
Injection
valve (V1)
1 7
6
2
3 45
Mixer
System
pumps
3 Wavelength
detector
Cond
Conductivity
monitor
pH
1 87
2
6
345
Sample
pump
1 87
2
6
345
Column
selection
valve (V2)
UV
pH detector
Fraction
collector
Frac-950
1 87
2
6
345
1 87
2
6
345
Buffer
select
valve (V6)
1 87
2
6
345
Sample selection
valve (V5)
Column
selection
valve (V3)
Outlet
valve (V4)
KTAexplorer 10 XT
In-line
filter
Injection
valve (V1)
1 7
6
2
3 45
Mixer
System
pumps
Column
selection
valve (V2)
UV
3 Wavelength
detector
Cond
Conductivity
monitor
pH
1 87
2
6
345
pH detector
Fraction
collector
Frac-950
1 87
2
6
345
Buffer
select
valve (V6)
1 87
2
6
345
1 87
2
6
345
Column
selection
valve (V3)
Outlet
valve (V4)
PUMP P-900
Mixer
Pump A
Pump B
Proportion
Valve
A2
Acid /Alkali
KTA 10 mls/min
KTA 100 mls/min
A1 B2
Buffer
B1
Salt
GRADIENT
<6 mls/min
6 -14 mls/min
<30 mls/min
30 - 50 mls/min
50 - 100 mls/min
Water
MIXER
0.6 ml mixer
2 ml mixer
2 mls mixer
5 mls mixer
12 mls mixer
Mixer
Pump B
Pump A
Switching
Valves
Buffer Select
Valve IV-908
A2
A1
2
3
A11
A12
A13
1 8
A14
B1
Water
7
5
A16
A15
B2
A17
A18
Salt
Common inlet
A2
Pump B
A1
Common inlet
Manual
Flowpath
Manual
A1 or A2
BufferValveA B1 or B2
B1
B2
Flowpath
BufferValveB
Open
Draw A
ir
out of
Buffer
Line
Syringe fitting
Wash OUT
IN-LINE
one-way
valve
Buffer
OUT
Ball outlet
check valve
High pressure
Piston seal
Piston
Backwash pump
diaphragm
Ball inlet
check valve
Wash
IN
Buffer
IN
ON-LINE FILTERS
P-903 ( 10 ml/min Pump )
Low Flow HPLC Filter
LOAD (sample)
INJECT (sample)
Column
Column
Column
2
3
Pump
Sample
Waste
Sample
PV-908
1
8
7
6
4
PV-908
1
Pump
7
3
6
4
PV-908
Pump
7
3
6
4
Waste
Sample
Waste
COLUMN
2
LOAD
SAMPLE
PUMP
Waste
Waste
LOAD
1
COLUMN
PUMP
3
4
6
5
Waste
Waste
INJECT
1
COLUMN
PUMP
3
4
Waste
6
5
Waste
WASTE
COLUMN
2
LOAD
SAMPLE
PUMP
3
6
5
4
Needle Port
Waste
Waste
Do Not Overtighten
COLUMN
LOAD
SAMPLE
PUMP
3
6
4
Luer Lock
Adapter
Waste
Waste
Superloop
SUPERLOOPS
10 ml
50 ml
Buffer
150 ml
Plunger
BUFFER
FLUSH
SAMPLE
LOAD
SAMPLE
INJECT
SAMPLE
FLUSH
WASTE
PUMP or
SYRINGE
COLUMN
COLUMN
Sample
Etched
Groove
Volume
Code No.
10 ml
50 ml
150 ml
4 Mpa
4 Mpa
2 Mpa
18-1113-81
18-1113-82
18-1023-85
Valve Seal
Ferrule
NeedleTube
Needle
Needle
Adapter
PEEK
PEEKInjection
InjectionValve
ValveBody
Body
PEEK
PEEKInjection
InjectionLuer
LuerAdaptor
Adaptor
Valve
ValveSeal
Seal
Valve
ValveChannel
ChannelPlate
Plate
Fluid Dynamics
Flow
PEEK Tubing
High Resistance
to Flow
Low Resistance
to Flow
End
Beginning
General
Fluid Dynamics
Bubble Segmentation
100ul Loop
Waste
50ul Load
60ul Load
100ul Load
200ul Load
Column
Load
Inject
Sample
Sample
pump
P-950
Pump
6
5
Waste
Column
Load
Inject
Pump
6
Buffer
Sample
Waste
Sample
Column
Load
Inject
Pump
6
PV-908
1
2
3
I/O
4
7
6
Wash
Samples
Waste
Column
1
2
Pump
Load
Inject
Injection valve
INV-907
7
3
Waste
6
5
PV-908
1
8
2
3
I/O
4
7
6
Samples
Wash
S3 S4 S5 S6
S8
Column
Pump
2
3
Inject
6
4
Load
Extra Tube
1/16 or 1/8
Tubing
Waste
Sample
Pump
Column
Extra Tube
Stop
Plug
MONITOR
Downward Flow
Position 1
1
7
Pump
3
4
Column
Extra Tube
Stop
Plug
MONITOR
Upward Flow
Position 2
DETECTOR
To Fraction
Collector
Default
F8
F1
F2
F3
I/O
F4
F5
F7
F6
F7
Waste
F8
F4
F3
F5
F6
250
500
mAU
Flowthrough
Fractionation
Inject
A1 A2 A3.
Eluate
Fractionation
0%B
100%B
Peak
Fractionation
End
Fraction Collection
Waste
Start
50
100
%B
HiTrap Connections
1/16 Female
1/16 Male
18-1112-55
1/16 Female
M6 Male
18-1112-57
Phenyl Sepharose
HiTrap Column
M6 Male
M6 Female
18-1112-58
1/16 Male
Phenyl Sepharose
Phenyl Sepharose
Phenyl Sepharose
Phenyl Sepharose
Phenyl Sepharose
Phenyl Sepharose
to
c
e
t
De
tor
c
e
t
De
cto
e
t
De