Molecular Biology: - Antibiotics

Molecular
Biology
Antibiotics
Buffers
Molecular Biology
Media
Reagents
Molecular Biology
PhytoTechnology
Product Number
Sigma Product
Number
Duchefa Product
Number
ew for 2008 are a line of Molecular

Biology/Plant Transformation products
from PhytoTechnology Laboratories.
This section is divided into four major groups:
Carbenicillin
C346
C3416
C1019
Carbenicillin, 100 mg/mL
C540
NA
NA
Media
Cefotaxime
C380
C7039
C0111
Biochemicals & Reagents
Cefotaxime, 100 mg/mL
C537
NA
NA
Hygromycin B in PBS
H370
NA
H0192
Timentin
T869
NA
DNA Denaturing Solution
D548
NA
NA
EDTA Solution, 0.5M
E582
E7889
NA
IPTG
I373
59740
I1401
MTT
M535
M2128
M1415
MUG
M569
M9130
M1404
Phosphinothricin, PPT
P679
45520
P0159
X-Gal
X874
B4252
X1402
X-Gluc, Ammonium
X877
B3783
X1405
X-Gluc, Sodium
X871
B5285
X1406
BIS-TRIS
B162
B4429
C1516
CHAPS
C526
C5070
C1374
HEPES
H326
H4034
H1504
MOPS-EDTA
M572
M5755
NA
SSC Buffer, 20x
S716
S6639
S1511
TRIS-Acetate EDTA, 50x
T769
T8280
NA
TBE
T831
T7527
T1507
TE
T855
93283
T1508
TRIS-Phosphate Buffer
T735
NA
NA
LB Broth, Lennox L
L585
L3522
L1703
LB Broth, Miller
L475
L3397
L1704
Terrific Broth
T754
T0918
NA
Product Description
Antibiotics
Buffers
All of the products are manufactured from

the highest purity raw materials available and
undergo the same rigorous quality control
testing as our other media and biochemicals.
Many of the products are further tested to be
Rnase/Dnase Free. Listed below are some
of the more commonly used products with
competitor cross-references.
Page 236 Molecular Biology
2008 PhytoTechnology Laboratories
A116 - Ampicillin, Sodium Salt
C380 - Cefotaxime, Sodium Salt
D(-)--Aminobenzylpenicillin, Sodium Salt

Powder
CAS Number: 69-52-3
Formula: C16H18N3NaO4S
Molecular Weight: 371.4
Soluble in Water
Tariff Code: 2941.10.1000
Store from 2 to 6 C
Merck 13, 591
(6R,7R)-3-[(Acetyloxy)methyl]-7-[[(2Z)-(2-amino-4thiazolyl)(methoxyimino)acetyl]amino]-8-oxo-5-thia-1azabicyclo[4.2.0]oct-2-ene-2-carboxylic Acid, Sodium Salt

Plant Tissue Culture Tested
Powder
CAS Number: 64485-93-4
Formula: C16H16N5O7S2Na
Soluble in Water
Store from 2 to 6 C
Merck 13, 1946
Available Package Sizes

5g
25 g
C540 - Carbenicillin Solution (100 mg/

mL)
-Carboxybenzylpenicillin, Disodium Salt
Aqueous Solution
Sterile Filtered
Liquid
Formula: C17H16N2O6SNa2
Miscible with Water
Store from -20 to 0 C

10 mL
100 mL
1g
5g
25 g
C537 - Cefotaxime Solution (100 mg/mL)

Aqueous Solution
Liquid
Formula: C16H16N5O7S2Na
Miscible with Water

10 mL
100 mL
C346 - Carbenicillin, Disodium Salt

-Carboxybenzylpenicillin, Disodium Salt
Powder
Formula: C17H16N2O6SNa2
Soluble in Water
Store from 2 to 6 C
Merck 13, 1801

5g
10 g
25 g
H397 - Hygromycin B
O-6-Amino-6-deoxy-L-glycero-D-galactoheptopyranosylidene-(12-3)-O--D-talopyranosyl-(15)
-2-deoxy-N3-methyl-D-Streptamine
From Streptomyces hygroscopicus
Average Activity: 1000 units/mg
Powder
Formula: C20H37N3O13
Soluble in Water or PBS Solution
Store from 2 to 6 C
Merck 13, 4878

1g
5g
Molecular Biology - Antibiotics
Page 237
MICROBIOLOGY
ANTIBIOTICS
MEDIA
Molecular Biology
ANTIBIOTICS
Molecular Biology
H385 - Hygromycin B Solution,
100 mg/mL in Distilled Water
Sterile Filtered
Liquid
Miscible with Water
Store from 2 to 6 C; Do Not Freeze
Merck 13, 4878
K378 - Kanamycin Monosulfate

O-3-Amino-3-deoxy--D-glucopyranosyl-(16)-O-[6amino-6-deoxy--D-glucopyranosyl-(14)]-2-deoxy-DStreptamine Monosulfate
Powder
Formula: C18H36N4O11 H2SO4
Soluble in Water
Store at Room Temperature
Merck 13, 5299

5g
10 g
25 g
10 mL
H370 - Hygromycin B Solution,

50 mg/mL in PBS Buffer
Sterile Filtered
Liquid
Miscible with Water
Store from 2 to 6 C; Do Not Freeze
Merck 13, 4878
K586 - Kanamycin Monosulfate Solution

(50 mg/mL)
Aqueous Solution
Sterile Filtered
Liquid
Miscible with Water
Store from 2 to 6 C

10 mL
100 mL

20 mL
Page 238 Molecular Biology - Antibiotics
T869 - Timentin
Ticarcillin Disodium Salt/ Potassium Clavulanate
mixture (15:1)
Powder
CAS Number: 4697-14-7 / 61177-45-5
Formula: C15H14N2Na2O6S2 / C8H8KNO5
Molecular Weight: 428.4 / 237.3
Soluble in Water
Store from 2 to 6 C

2g
5g
10 g
25 g
T767 - Timentin Solution (100 mg/mL)

Ticarcillin Disodium Salt/Potassium Clavulanate Mixture
Aqueous Solution
Sterile Filtered
Liquid
Miscible with Water

10 mL
100 mL
Molecular Biology - Antibiotics
Page 239
ANTIBIOTICS
Molecular Biology
BUFFERS
Molecular Biology
B157 - BES
H326 - HEPES, Free Acid
N,N-bis(2-Hydroxyethyl)-2-aminoethanesulfonic acid
Useful pH range: 6.4-7.8
pKa @ 25 C = 7.1
Powder
Formula: C6H15NO5S
Soluble in Water
N-(2-Hydroxyethyl)Piperazine-N-(2-Ethanesulfonic Acid);
4-(2-Hydroxyethyl)-1-Piperazineethanesulfonic Acid
Molecular Biology Grade
Effective Buffering Range: pH 6.8 to 8.2
Powder
Formula: C8H18N2O4S
Soluble in Water
500 g
1 Kg
500 g
1 Kg
B162 - BIS-TRIS
M825 - MES Monohydrate
2,2-Bis-(hydroxymethyl)-2,2,2-nitrilotriethanol
pKa @ 25 C = 6.5
Powder
Formula: C8H19NO5
Soluble in Water
2-(N-Morpholino)ethanesulfonic Acid
Powder
CAS Number: 145224-94-8
Formula: C6H13NO4SH2O
Soluble in Water
Merck 13, 5929
500 g
1 Kg
100 g
500 g
1 Kg
C526 - CHAPS
M631 - MOPS
3-[(3-Cholamidopropyl)dimethylammonio]-1propanesulfonate
CHAPS is a nondenaturing zwitterionic detergent that is
typically used to solubilize membrane proteins.
Powder
Formula: C32H58N2O7S
Soluble in Water
Merck 13, 2052

1g
5g
Page 240 Molecular Biology - Buffers
3-[N-Morpholino]propanesulfonic acid, Free Acid

pKa @ 25 C = 7.15
Powder
Formula: C7H15NO4S
Soluble in Water
Merck 13, 6288

500 g
1 Kg
M572 - MOPS-EDTA-Sodium Acetate

Buffer (10x)
83.71
8.20
3.72
100 g
500 g
1 Kg
Sterile Filtered
DNase/RNase Free
Miscible with Water
Components (g/L)
Sodium Chloride (3 M)
Sodium Citrate Dihydrate (0.3 M)
Contains sufficient HCl to adjust pH to 7.0
175.32
88.23

500 mL
1L
S677 - SSPE Buffer Solution (10x)
P711 - PIPES, Sesquisodium Salt

Piperazine-N,N-bis(2-ethanesulfonic Acid), Sesquisodium
Salt
pKa @ 25 C = 6.8
Powder
CAS Number: 100037-69-2
Formula: C8H16.5N2O6S2Na1.5
Soluble in Water
Merck 13, 7561

100 g
500 g
1 Kg
Sterile Filtered
DNase/RNase Tested
Liquid
Miscible with Water
Components (g/L)
Sodium Chloride (3.0 M)
Sodium Phosphate Monobasic (0.2 M)
Disodium EDTA (0.02 M)
87.65
13.80
3.7

500 mL
1L
T831 - Tris Borate EDTA Buffer

TBE Buffer
Use 17.02 grams to make 1 liter of 1x buffer solution
Powder
Soluble in Water
Components (g/L)
Tris Base
10.78
Boric Acid
5.5
Na2EDTA2H2O
0.74
100 g
500 g
Molecular Biology - Buffers
Page 241
BUFFERS
MESA Buffer
Powder
Soluble in Water
Components (g/L)
MOPS (0.4 M)
Sodium Acetate3H2O (0.1 M)
Na2EDTA2H2O (0.01 M)
S716 - SSC Buffer Solution (20x), pH 7.0
ANTIBIOTICS
Molecular Biology
Molecular Biology
BUFFERS
T855 - Tris EDTA Buffer

TE Buffer
Powder
Soluble in Water
Components (g/L)
Tris Base
1.21
Na2EDTA2H2O
0.37
100 g
500 g
T769 - Tris Acetate EDTA Buffer

Solution (50x), pH 8.1 - 8.5
TAE Buffer
Sterile Filtered
DNase/RNase Tested
Liquid
Miscible with Water
Components (g/L)
Tris Base (2.0 M)
EDTA, Free Acid (0.05 M)
Gacial Acetic Acid (1.0 M)
242.28
14.61
57.1 mL
T814 - Tris Glycine Buffer

TG Buffer
Powder
Soluble in Water
Components (g/L)
Tris Base
3.03
Glycine
14.41

100 g
500 g
500 mL
1L
T838 - Tris Base

2-Amino-2-hydroxymethyl-1,3-propanediol
Powder
CAS Number: 77-86-1
Formula: C4H11NO3
Soluble in Water
T821 - Tris Glycine SDS Buffer

TG-SDS Buffer
Powder
Soluble in Water
Components (g/L)
Glycine
14.41
Sodium Dodecyl Sulfate
1.00

100 g
500 g
100 g
500 g
1 Kg
5 Kg
T774 - Tris Borate EDTA Buffer Solution

(10x)
TBE Buffer
Sterile Filtered
DNase/RNase Tested
Liquid
Miscible with Water
Components (g/L)
Tris Base (0.89 M)
Boric Acid (0.89 M)
107.8
55.0
7.4
500 mL
1L
T749 - Tris Glycine SDS Buffer Solution

(10x)
TBE Buffer
Sterile Filtered
DNase/RNase Tested
Liquid
Miscible with Water
Components (g/L)
Tris Base (0.44 M)
Boric Acid (0.44 M)
Protein Running Buffer; TG-SDS Buffer

Sterile Filtered
DNase/RNase Tested
Liquid
Miscible with Water
Components (g/L)
Tris Base (0.25 M)
Glycine (1.92 M)
Sodium Dodecyl Sulfate (0.04 M)
53.9
27.5
3.7
30.28
144.14
10.0
500 mL
1L
500 mL
1L
T752 - Tris EDTA Buffer Solution,

pH 8.0
T764 - Tris HCl Solution 1.0 M, pH 7.5
TE Buffer
Sterile Filtered
DNase/RNase Tested
Liquid
Miscible with Water
Components (g/L)
Tris Base (0.01 M)
1.21
0.37
Sterile Filtered
DNase/RNase Tested
Liquid
Miscible with Water
Components (g/L)
Tris Base (1.0 M)
121.14

500 mL
1L
500 mL
1L
Molecular Biology - Buffers
Page 243
BUFFERS
T773 - Tris Borate EDTA Buffer Solution

(5x)
ANTIBIOTICS
Molecular Biology
Molecular Biology
T858 - Tris Hydrochloride
2-Amino-2-hydroxymethyl-1,3-propanediol Hydrochloride
BUFFERS
Powder
Formula: C4H11NO3HCl
Soluble in Water

100 g
500 g
1 Kg
T735 - TPE Buffer Solution (10x)

Tris-Phosphate-EDTA Buffer Solution
Sterile Filtered
DNase/RNase Tested
Liquid
Miscible with Water
Components (g/L)
Tris-Phosphate (0.8 M)
EDTA (0.02 M)
1,753
58.5

500 mL
1L
H289 - Hanahans Broth
L465 - LB Agar
SOB Medium was developed by Hanahan (J. Mol. Biol. 155:

557, 1983) for the cultivation of competent E. coli cells prior
to transformation. This is a nutritionally rich medium to
which 22 mM (0.4%) D-glucose is added for the final stage
of transformation; this allows the E. coli cells to mend the
performations and replicate.
Store from 2 to 6 C
Soluble in Water
Use at 28 grams per liter
Components (g/L)
Casein Enzymatic Hydrolysate
20.00
Yeast Extract
5.00
Magnesium Sulfate
2.40
Sodium Chloride
0.50
Potassium Chloride
0.186
pH at Room Temperature
6.8 - 7.2
Luria-Bertani Agar
Miller Modification
Store from 2 to 6 C
Soluble in Water
Use at 25.00 grams per liter
Components (g/L)
Agar
Sodium Chloride
Tryptone
Yeast Extract

500 g
5 Kg
L591 - LB Broth Solution w/ Glycerol

Luria-Bertani Broth
Lennox L Modification
Sterile Filtered
Store from 2 to 6 C
500 g
5 Kg
L552 - LB Agar
Luria-Bertani Agar
Store from 2 to 6 C
Soluble in Water (Partially)
Components (g/L)
Sodium Chloride
Agar
Tryptone
Yeast Extract
12.0
10.0
10.0
5.0
7.0 0.5
Components (g/L)
5.0
12.0
10.0
5.0
Not Established
Glycerol
Sodium Chloride
Tryptone
Yeast Extract
4.0 mL
5.0
10.0
5.0
Not Established

100 mL
500 mL
1L
500 g
5 Kg
Molecular Biology - Microbiology Media
Page 245
MICROBIOLOGY
MEDIA
ANTIBIOTICS
Molecular Biology
Molecular Biology
L585 - LB Broth Solution
L475 - LB Broth
Luria-Bertani Broth
Sterile Filtered
Store from 2 to 6 C
Luria-Bertani Broth
Miller Modification
Store from 2 to 6 C
Soluble in Water
Components (g/L)
Sodium Chloride
Tryptone
Yeast Extract
Components (g/L)
Sodium Chloride
Tryptone
Yeast Extract
5.0
10.0
5.0
Not Established
MICROBIOLOGY
MEDIA
10.0
10.0
5.0
7.0 0.5
100 mL
500 mL
1L
500 g
5 Kg
L543 - LB Broth
Sterile Filtered
Typically used in the recovery step of Escherichia coli
competent cell transformations.
Store from 2 to 6 C
Luria-Bertani Broth
Store from 2 to 6 C
Soluble in Water
Components (g/L)
Sodium Chloride
Tryptone
Yeast Extract
S657 - SOC Medium Solution
5.0
10.0
5.0
Not Established

500 g
5 Kg
Page 246 Molecular Biology - Microbiology Media
Components (g/L)
Glucose, Anhydrous
Magnesium Sulfate, Anhydrous
Potassium Chloride
Sodium Chloride
Tryptone
Yeast Extract
3.6032
2.4074
0.1864
0.5026
20.0
5.0

100 mL
500 mL
1L
T760 - Terrific Broth
Y889 - YEP Medium
Requires the addition of 4.0 mL of Glycerol (Product

Number G381) per liter of broth.
Store from 2 to 6 C
Soluble in Water
Components (g/L)
Tryptone
11.8
Potassium Phosphate, Dibasic, Anhydrous
9.4
Potassium Phosphate, Monobasic, Anhydrous 2.2
Yeast Extract
23.6
Not Established
Yeast Extract Peptone Medium

Store from 2 to 6 C
Components (mg/L)
Agar
Peptone from Meat
Sodium Chloride
Yeast Extract
500 g
5 Kg
Y893 - YMB Medium
Sterile Filtered
Store at 2 to 6 C
Components (g/L)
Glycerol
4.0 mL
Tryptone
11.8
9.4
Potassium Phosphate, Monobasic, Anhydrous 2.2
Yeast Extract
23.6
Not Established
15,000
10,000
5,000
10,000
Not Established

500 g
Yeast Mannitol Broth Medium

Store from 2 to 6 C
Components (mg/L)
Magnesium Sulfate, Anhydrous
Agar
D-Mannitol
Sodium Chloride
Yeast Extract
200
500
15,000
10,000
100
400
Not Established

500 g

100 mL
500 mL
1L
Molecular Biology - Microbiology Media
Page 247
MICROBIOLOGY
MEDIA
T754 - Terrific Broth Solution

w/ Glycerol
ANTIBIOTICS
Molecular Biology
Molecular Biology
A104 - Acetosyringone
B441 - Bradford Reagent
4-Hydroxy-3,5-dimethoxyacetophenone
This material is sensitive to temperature and should not be
autoclaved.
Powder
Formula: C10H12O4
Soluble in DMSO
Used to determine concentration of proteins in solution based

on the formation of a Brilliant Blue G - protein complex.
Useful in determining the protein content of cell fractions
and for gel exectrophoresis.
Liquid
Store from 2 to 6 C

1g
5g
25 g
A115 - Agarose
Low EEO
Powder
Formula: (C12H14O5(OH)4)n
Soluble in Hot Water
REAGENTS

100 g
500 g
B496 - Bicinchoninic Acid Solution

BCA
Typically used for determination of protein concentration.
Liquid
Miscible with Water

10 mL
100 mL

10 mL
100 mL
C277 - Citric Acid Anhydrous

2-Hydroxy-1,2,3-propanetricarboxylic Acid
Powder
CAS Number: 77-92-9
Formula: C6H8O7
Soluble in Water
Merck 13, 2350

500 g
1 Kg
C466 - Cupric Sulfate Solution (4%)

Copper(II) Sulfate Pentahydrate
Typically used with Bicinchoninic Acid Solution (B496) in
the determination of protein concentration.
Liquid
Formula: CuSO45H2O
Miscible with Water
Merck 13, 2682
Components (g/L)
Cupric Sulfate5H2O
40.0
10 mL
100 mL
Page 248 Molecular Biology - Reagents
D241- Dimethyl Sulfoxide
E316 - Ethylenediaminetetraacetic Acid,

Free Acid
DMSO; Methyl Sulfoxide

Solidifies below and melts above 18.5 C.
Liquid
CAS Number: 67-68-5
Formula: (CH3)2SO
Miscible with Water
Merck 13, 3285
EDTA; Edetic Acid

Powder
CAS Number: 60-00-4
Formula: C10H16N2O8
Soluble in KOH
Merck 13, 3545
500 mL
1L
4L
100 g
500 g
1 Kg
D548 - DNA Denaturing Solution
E582 - EDTA Solution 0.5 M, pH 8.0
Sterile Filtered
Liquid
Miscible with Water
Components (g/L)
Sodium Hydroxide (0.5 M)
20.0
87.66
100 mL
500 mL
1L
Sterile Filtered
DNase/RNase Tested
Liquid
Formula: C10H14N2O8Na22H2O
Miscible with Water
Merck 13, 3543
Components (g/L)
Na2EDTA2H2O (0.5 M)
Contains sufficient NaOH to adjust pH to 8.0
186.12
D544 - DNA Neutralizing Solution,

pH 7.4
Sterile Filtered
Liquid
Miscible with Water
Components (g/L)
Tris Base (1.0 M)
500 mL
1L
121.14
87.66

500 mL
1L
Molecular Biology - Reagents
Page 249
REAGENTS
ANTIBIOTICS
Molecular Biology
Molecular Biology
G403 - Gel Loading Buffer Solution
H245 - Hydrochloric Acid Solution 1.0 N
Sterile Filtered
DNase/RNase Tested
Recommended usage: Add 1 volume gel
loading buffer to 2 - 5 volumes of sample and
mix well.
Liquid
Miscible with Water
Components (g/L)
Bromophenol Blue (0.05% (w/v))
Sucrose (40% (w/v))
Na2EDTA2H2O (0.1 M)
SDS (0.5% (w/v))
Hydrogen Chloride Solution 1.0 N; Muriatic Acid

Contains about 15% concentrated Hydrochloric Acid per liter
Liquid
Formula: HCl
Miscible with Water
Merck 13, 4801
0.50
400
37.224
5.0

100 mL
500 mL
1L
REAGENTS
H276 - Hexadecyltrimethylammonium
Bromide
CTAB, HTAB, Centimide, Cetrimide, Cetrimonium bromide,
N,N,N-trimethyl-1-hexadecanaminium bromide
DNase/RNase Tested
Powder
CAS Number: 59-09-0
Formula: CH3(CH2)15N(Br)(CH3)3
Soluble in Water

500 mL
1L
4L
I373 - Isopropyl--Dthiogalactopyranoside
IPTG
Powder
Formula: C9H18O5S
Soluble in Water
Store from 2 to 6 C

1g
5g
25 g
100 g
500 g
M649 - 2-Mercaptoethanol
P621 - Percoll
-Mercaptoethanol, 2-Me, 2-Thioethanol, Thioglycol, 2Hydroxyethylmercaptan

Density: 1.114 g/mL at 20 C.
Concentration (Pure): 14.3 M
DNase, RNase, & Protease Tested
Liquid
CAS Number: 60-24-2
Formula: HSCH2CH2OH
Miscible with Water
Centricoll; pH ~8.9 at 20 C
Percoll is a 23% (w/w) aqueous solution of
15-30 nm colloidal silica particles coated with
PVP (polyvinylpyrrolidone). Percoll is typically
used for the separation of cells and subcellular
particles.

10 mL
100 mL
ANTIBIOTICS
Molecular Biology
Percoll is a registered trademark of GE

Healthcare.
Liquid
Miscible with Water
Store from 2 to 6 C

10 mL
M565 - MTT
3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium
Bromide; Thiazolyl Blue Tetrazolium Bromide
Powder
Formula: C18H16N5SBr
Soluble in Water
Store from 2 to 6 C
Monoammonium Salt; PPT;

Glufosinate Ammonium;
2-Amino-4-(Hydroxymethylphosphinyl)Butanoic Acid,
Monoammonium Salt
1.0 mg/mL Aqueous Solution
Sterile Filtered
Liquid
Formula: C5H15NO4P
Miscible with Water
M569 - MUG
10 mL
100 mL
4-Methylumbelliferyl--D-glucuronide Trihydrate
Powder
Formula: C16H16O93H2O
Soluble in DMSO

100 mg
1g
Page 251
REAGENTS
1g
5g
10 g
G523 - DL-Phosphinothricin Solution
Molecular Biology
P679 - DL-Phosphinothricin,
Monoammonium Salt
P730 - Potassium Chloride Solution

3.0 M
PPT; 2-Amino-4-(hydroxymethylphosphinyl)butanoic Acid,

Monoammonium Salt; Glufosinate Ammonium
Powder
Formula: C5H15N2O4P
Soluble in Water
Merck 13, 7425
Electrode Filling/ Soaking Solution

Liquid
Formula: KCl
Miscible with Water
Merck 13, 7704

250 mg
1g
REAGENTS
P671 - Potassium Acetate Solution 3.0 M,

pH 5.0
Sterile Filtered
Liquid
Formula: KC2H3O2
Miscible with Water
Merck 13, 7687
Components (g/L)
Potassium Acetate
294.42

100 mL
500 mL
1L

100 mL
500 mL
P682 - Potassium Hydroxide Solution

1.0 N
Liquid
Formula: KOH
Miscible with Water
Merck 13, 7724

500 mL
1L
4L
S722 - Sodium Acetate Solution 3.0 M,

pH 5.2
Sterile Filtered
Liquid
Formula: C2H3NaO2
Miscible with Water
Merck 13, 8642
Components (g/L)
Sodium Acetate3H2O
408.1

500 mL
1L
S740 - SDS Solution (10%)
U890 - Urea
Sterile Filtered
DNase/RNase Tested
Liquid
Formula: C12H25NaO4S
Miscible with Water
Merck 13, 8710
Components (g/L)
Sodium Dodecyl Sulfate
Carbonyldiamide; Carbamide
Powder
CAS Number: 57-13-6
Formula: CH4N2O
Soluble in Water
Merck 13, 9935
100

100 mL
500 mL
1L
S844 - Sodium Dodecyl Sulfate

SDS; Sodium Lauryl Sulfate
Powder
Formula: C12H25NaO4S
Soluble in Water
Merck 13, 8710

500 g
1 Kg
5 Kg
W783 - Water, Tissue Culture/

Sterile Filtered
DNase/RNase Tested
Liquid
Formula: H2O
Merck 13, 10098

1L
3.9 L
S829 - D-Sucrose, Ultra-Pure
5-Bromo-4-chloro-3-indolyl--D-galactopyranoside
Powder
Formula: C14H15BrClNO6
Soluble in DMSO or KOH

500 g
1 Kg
5 Kg
25 Kg
X874 - X-Gal

100 mg
500 mg
1g
Page 253
REAGENTS
25 g
100 g
500 g
-D-Fructofuranosyl--D-glucopyranoside; Cane Sugar

Powder
CAS Number: 57-50-1
Formula: C12H22O11
Soluble in Water
Merck 13, 8966
ANTIBIOTICS
Molecular Biology
Molecular Biology
X884 - X-Gal, Blue-Gal
X763 - X-Gluc, Salmon
5-Bromo-3-indolyl--D-galactopyranoside
Powder
Formula: C14H16BrNO6
6-Chloro-3-indolyl--D-glucluronic Acid,
Cyclohexylammonium Salt; Salmon X-GlucA,
Cyclohexylammonium Salt
Powder
CAS Number: 138182-20-4
Formula: C20H27N2O7Cl
Store at -20 to 0 C

100 mg
500 mg
1g
X751 - X-Gluc, Magenta-Gluc,

Monocyclohexyl Ammonium Salt
Magenta-GlcA
This compound produces a magenta color in GUS+ colonies;
an alternative to X-Gluc
Powder
CAS Number: 144110-43-0
Formula: C20H26BrClN2O7
Store from -20 to 0
REAGENTS

100 mg
1g
X877 - X-Gluc, Monocyclohexyl

Ammonium Salt
100 mg
500 mg
1g
X871 - X-Gluc, Sodium Salt

5-Bromo-4-chloro-3-indolyl--D-glucuronic Acid, Sodium
Salt
Powder
CAS Number: 129541-41-9
Formula: C14H12BrClNaNO7

100 mg
500 mg
1g
5g
X-GlcA; 5-Bromo-4-chloro-3-indolyl--D-glucuronic Acid,

Monocyclohexyl Ammonium Salt
Powder
CAS Number: 114162-64-0
Formula: C20H26BrClN2O7

100 mg
500 mg
1g
5g

Molecular Biology: - Antibiotics

Uploaded by

Document Information

Original Description:

Original Title

Copyright

Available Formats

Share this document

Share or Embed Document

Sharing Options

Did you find this document useful?

Is this content inappropriate?

Copyright:

Available Formats

Molecular Biology: - Antibiotics

Uploaded by

Copyright:

Available Formats

Molecular

ew for 2008 are a line of Molecular

Carbenicillin, 100 mg/mL

Biochemicals & Reagents

Cefotaxime, 100 mg/mL

DNA Denaturing Solution

EDTA Solution, 0.5M

SSC Buffer, 20x

TRIS-Acetate EDTA, 50x

All of the products are manufactured from

Page 236 Molecular Biology

2008 PhytoTechnology Laboratories

A116 - Ampicillin, Sodium Salt

C380 - Cefotaxime, Sodium Salt

D(-)--Aminobenzylpenicillin, Sodium Salt

(6R,7R)-3-[(Acetyloxy)methyl]-7-[[(2Z)-(2-amino-4thiazolyl)(methoxyimino)acetyl]amino]-8-oxo-5-thia-1azabicyclo[4.2.0]oct-2-ene-2-carboxylic Acid, Sodium Salt

Available Package Sizes

Available Package Sizes

C540 - Carbenicillin Solution (100 mg/

Available Package Sizes

C537 - Cefotaxime Solution (100 mg/mL)

Available Package Sizes

C346 - Carbenicillin, Disodium Salt

Available Package Sizes

Available Package Sizes

2008 PhytoTechnology Laboratories