BRIGHT FIELD MICROSCOPY

ANDRES, JAMAICA
ASUNCION, CRISTINE
BOY, ELLEN KRISTINE

CORPUZ, DANICA
DAILEG, JUDIMEL
SANTOS, VIRGILIO

BRIGHT FIELD
MICROSCOPY

SIMPLEST OPTICAL MICROSCOPY ILLUMINATION

TECHNIQUE

IT FORMS A DARK IMAGE AGAINST BRIGHT

BACKGROUND

USES VISIBLE LIGHT AS SOURCE OF ILLUMINATION

COMMONLY USED TO VIEW LIVE AND IMMOBILE

SPECIMENS SUCH AS BACTERIA, CELLS, AND
TISSUES.

BRIGHT FIELD
MICROSCOPY

STAINING OF ORGANISM IS REQUIRED

LIGHT SOURCE: EITHER A MIRROR OR AN ELECTRONIC

ILLUMINATOR LOCATED IN THE BASE
IMAGE FORMED: RELATIVELY LARGE INTERNAL STRUCTURES
AN OUTLINE CAN BE SEEN
TOTAL MAGNIFICATION: *IF 10X OCULAR MAGNIFICATION IS
USED)
RANGE: 10X 1000X

HOW IMAGE IS FORMED:

DARK FIELD
MICROSCOPY

IT USES A DARK BACKGROUND WITH THE

SPECIMEN BEING THE ONLY ONE ILLUMINATED.

USED IN OBSERVING UNSTAINED SPECIMENS

LIGHT SOURCE: LIGHT BULB FROM A MICROSCOPE

IMAGE FORMED: DARK BACKGROUND WITH ILLUMINATED
SPECIMEN
TOTAL MAGNIFICATION: CAN RANGE FROM THOSE OF
COMPOUND MICROSCOPE (10X TO 1000X)

ONLY LIGHT THAT HAS BEEN REFLECTED

OR REFRACTED BY THE SPECIMEN FORMS
THE IMAGE

THE DARK FIELD MICROSCOPE CAN RAVEL

CONSIDERABLE INTERNAL STRUCTURE IN
LARGER EUKARYOTUC MICROORGANISM

HOW IMAGE IS FORMED: