METHODS AND MATERIALS

Mung bean (Vigna radiata) seeds were immersed in water wherein the
floating unhealthy seeds were discarded and the sunken viable seeds were used in
the experiment. There were five different set-ups and each tested the effects of (a)
temperature, (b) pH, (c) osmotic gradient, (d) light and of (e) hormones. 14 Petri
plates were cleaned, lined up with cotton and equally distributed with ten mung
beans. For (a), three Petri plates were wet with distilled water, saturating the cotton.
Each plate was put in a room, low (refrigerator) and high (incubator, 30)
temperatures. For (b), three plates were wet with solutions of pH 3.0, pH 11.0 and
distilled water. For (c), three plates were wet with NaCl solutions having varying
concentrations of 0.5g NaCl/200ml, 2g NaCl/150ml, and of 5g NaCl/150ml. For (d),
two plates were wet with distilled water. One plate was set on a normally lighted
place while the other one was in a dark place. Lastly, for (e), three plates were wet
with distilled water (control), 20ppm gibberellic acid (GA), and with 20ppm indole
acetic acid (IAA) solution. The plates were placed in a dark area. All the plates in
each set-up were labeled accordingly. After two days, percent germination of each
set-up was observed then five days, the lengths of hypocotyl-root axis were
measured using a ruler. Seeds are considered germinated if its radicle emerged
from the seed coat. The findings were summarized and analyzed using statistical
methods.