TRANSCRIPTION

Describe initiation.

TRANSCRIPTION
RNA polymerase
Prokaryotes
Eukaryotes (pol I-III)

Transcription unit

Direction of elongation

Stages: Initiation

Stages: Elongation

Stages: Elongation

What are the 2 events that occur simultaneously in this picture?

In what organism can this happen? Prokaryote or eukaryote?

Stages: Termination
Prokaryotes:
end of
termination
signal
Eukaryotes:
past the
signal
AAUAAA

Which is a prokaryotic cell? Eukaryotic cell?

Spot the differences.
What occurs during RNA processing?

RNA Processing

RNA Splicing
Split genes

Aside from removing introns, what else happens during processing?

 Both ends of pre-mRNA are usually

altered

Interior sections cut out

Splicing of remaining parts
*In eukaryotes

 5 end (5 cap)

3 end (Poly (A) tail)

Functions:
Protects mRNA from degradation
Ribosome binding
Facilitate export of mRNA from nucleus

 Small nuclear ribonucleoproteins (snRNPs)

Recognize splice sites
Spliceosome
Cuts & pastes at splice sites

What are the 2 components of snRNPs?