Standardization of Vibrio campbelli doses

Artemia were challenged with following doses of VC (0, 10 4, 105, 106 and 107) for 24, 36 and 48 h, respectively. They
Treatment Intial no
Control
30
Control
30
Control
30
4
10
30
10 4
30
4
10
30
5
10
30
10 5
30
5
10
30
10 6
30
6
10
30
10 6
30
7
10
30
10 7
30
7
10
30

24 h
28
29
30
30
28
29
23
30
28
29
30
26
28
28

Survival %
93.33
96.67
0.00
100.00
100.00
93.33
96.67
76.67
100.00
93.33
96.67
100.00
86.67
93.33
93.33

36 h Survival %
15
50.00
10
33.33
0.00
24
80.00
22
73.33
26
86.67
21
70.00
12
40.00
14
46.67
25
83.33
27
90.00
27
90.00
26
86.67
26
86.67
28
93.33

48 h
0

2
1
0
2
3
3
2
4
1
6
3
15

Survival %
0.00
0.00
0.00
6.67
3.33
0.00
6.67
10.00
10.00
6.67
13.33
3.33
20.00
10.00
50.00

Experiment 2 (Standardization of VC) - same bottle was used for different hours

Treatment Initial
Control
Control
Control
Control
Control
10 2
10 2
10 2
10 2
10 2
10 4
10 4
10 4
10 4
10 4
10 5
10 5
10 5
10 5
10 5
10 6
10 6
10 6

24 h
30
30
30
30
30
30
30
30
30
30
30
30
30
30
30
30
30
30
30
30
30
30
30

29
28
29
30
30
27
29
28
25
29
29
29
27
28
29
27
29
28
28
27
26
28
29

Survival 36 h
96.67
93.33
96.67
100.00
100.00
90.00
96.67
93.33
83.33
96.67
96.67
96.67
90.00
93.33
96.67
90.00
96.67
93.33
93.33
90.00
86.67
93.33
96.67

28
29
26
27
29
25
27
25
20
29
28
25
27
28
28
28
26
28
27
28
20
25
25

Survial
48 h
93.33
96.67
86.67
90.00
96.67
83.33
90.00
83.33
66.67
96.67
93.33
83.33
90.00
93.33
93.33
93.33
86.67
93.33
90.00
93.33
66.67
83.33
83.33

27
12
17
26
17
12
11
11
22
18
15
12
17
12
15
6
11
2
12
7
4
4
8

Survival
90.00
40.00
56.67
86.67
56.67
40.00
36.67
36.67
73.33
60.00
50.00
40.00
56.67
40.00
50.00
20.00
36.67
6.67
40.00
23.33
13.33
13.33
26.67

In this experiment differ

10 6
10 6
10 7
10 7
10 7
10 7
10 7

30
30
30
30
30
30
30

26
23
26
25
29
28
28

86.67
76.67
86.67
83.33
96.67
93.33
93.33

28
21
17
19
25
25
25

93.33
70.00
56.67
63.33
83.33
83.33
83.33

11
4
10
4
4
8
5

36.67
13.33
33.33
13.33
13.33
26.67
16.67

48 h, respectively. They are also fed with autoclave LVS3 @ 10 7 cells per mL
In this experiment different bottles were used for different hours.

----------

OD

OD

OD

Control
37-40
30
1
2
3
HSpr14-6
Hsp 70 - 24/11/2011
HSP70 29/11/2011
Hsp70 - 31/1/2012
Mu cTinh-4
Mu cTinh-5
Mu cTinh-6
Mau A Trung (Ei)
N0.1 (cTinh)
N0.5 (cTinh)
N0.6 (cTinh)

0.401
0.378
0.363
0.413
0.419
0.431
0.136
0.231
0.252
0.3
0.15
0.344
0.018
0.357
0.254
0.365
0.352
0.2

0.447
0.406
0.407
0.407
0.379
0.375
0.146
0.229
0.255
0.351

E1-omp48 4-4-14
E2-omp48 4-4-14
E1-omp

0.127
0.107
0.085
0.191
0.031
0.16
0.038
0.051
0.02

OD
0.417
0.323
0.325
0.443
0.4
0.405
0.146
0.214
0.269
0.299

0.424
0.429
0.351
0.412
0.373
0.427
0.145

0.131
0.109

0.132
0.111

0.137
0.113

0.185
0.032
0.162
0.043
0.06
0.017

0.186
0.033
0.171
0.054
0.065
0.02

0.192
0.033

0.314

0.314
0.014

y = 0,0299x + 0,0128

average
0.422
0.384
0.362
0.419
0.393
0.410
0.143
0.225
0.259
0.316
0.150
0.329
0.016
0.357
0.254
0.365
0.352
0.200
0.132
0.110
0.085
0.189
0.032
0.164
0.045
0.059
0.019

Protein content (ug/100ul)

13.6939799331
12.4147157191
11.6622073579
13.5769230769
12.7073578595
13.2675585284
4.3628762542
7.0858416945
8.2229654404
10.1404682274
4.5886287625
10.5752508361
0.1070234114
11.5117056856
8.0668896321
11.779264214
11.3444816054
6.2608695652

dilution
100
100
100
100
100
100
10
2.5
5
5
1.6666666667
3.3333333333
3.3333333333
1
1.6666666667
5
1.6666666667
10

3.9782608696
3.2508361204
2.4147157191
5.8762541806
0.6505016722
5.0680044593
1.0769230769
1.5340022297
0.2073578595

1
1

Protein content (ug/100ul)

1369.397993311
1241.4715719064
1166.220735786
1357.6923076923
1270.7357859532
1326.7558528428
43.6287625418
17.7146042363
41.1148272018
50.7023411371
7.6477146042
35.2508361204
0.3567447046
11.5117056856
13.4448160535
58.8963210702
18.9074693423
62.6086956522

Protein content (ug/ul)

13.6939799331
12.4147157191
11.6622073579
13.5769230769
12.7073578595
13.2675585284
0.4362876254
0.1771460424
0.411148272
0.5070234114
0.076477146
0.3525083612
0.003567447
0.1151170569
0.1344481605
0.5889632107
0.1890746934
0.6260869565

3.9782608696
3.2508361204

0.0397826087
0.0325083612

For 15 g sample
Sample
1.1
1.2
1.3
1.1
1.2
1.1
10 ul

DW
8.9
8.8
8.7
8.9
8.8
8.9
90 ul
40
20
20
60
30.0
30.0
100.0
60.0
20.0
60.0
10.0
100.0
100.0

60
80
80
40
70
70
0
40
80
40
90
0
0

Total
10
10
10
10
10
10
100 ul
100
100
100
100
100
100
100
100
100
100
100
100
100

y = 0,0299x + 0,0128

OD
Control
37-40
30
1
2
3

OD
0.287
0.304
0.37
0.284
0.336
0.346

OD
0.321
0.313
0.434
0.251
0.265
0.352

OD
0.287
0.336
0.347
0.238
0.25
0.334

0.331
0.263
0.329
0.244
0.29
0.346

average
0.307
0.304
0.370
0.254
0.285
0.345
#DIV/0!

Protein content (ug/100ul)

9.8227424749
9.7391304348
11.9464882943
8.0752508361
9.1120401338
11.0936454849

dilution
100
100
100
100
100
100

For 15 g sample
Protein content (ug/100ul)Protein content (ug/ul)
Sample
DW
982.2742474916
9.8227424749
1.5
8.5
973.9130434783
9.7391304348
1.5
8.5
1194.6488294314
11.9464882943
1.3
8.7
807.525083612
8.0752508361
1.9
8.1
911.2040133779
9.1120401338
1.6
8.4
1109.364548495
11.0936454849
1.4
8.6

Total
10
10
10
10
10
10

HS/NHS
0.436
10
22.93578
200
4587.156

ug/ul
ug/fish
ul/fish
fish
ul

24/11/2011
0.18 ug/ul
10 ug/fish
55.56 ul/fish
80 fish
4444.44 ul

11/11/2011
0.4
10
25.00
80
2000.00

ug/ul
ug/fish
ul/fish
fish
ul

Hsp70 tim vo tm

V hsp70 cn

Hsp70 tim vo tm

V hsp70 cn

Hsp70 tim vo tm

V hsp70 cn

0.51
5
9.80
7
68.63
1400
1331.37

ug/ul
ug/0.2ml/shrimp
ul/shrimp
no. of shrimp
ul
V tong (ul)
V pbs (ul)

0.51
6
11.76
7
82.35
1400
1317.65

ug/ul
ug/0.2ml/shrimp
ul/shrimp
no. of shrimp
ul
V tong (ul)
V pbs (ul)

0.51
7
13.73
7
96.08
1400
1303.92

ug/ul
ug/0.2ml/shrimp
ul/shrimp
no. of shrimp
ul
V tong (ul)
V pbs (ul)

0.51
8
15.69
7
109.80
1400
1290.20

ug/ul
ug/0.2ml/shrimp
ul/shrimp
no. of shrimp
ul
V tong (ul)
V pbs (ul)

0.51
9
17.65
7
123.53
1400
1276.47

ug/ul
ug/0.2ml/shrimp
ul/shrimp
no. of shrimp
ul
V tong (ul)
V pbs (ul)

0.51
10
19.61
7
137.25
1400
1262.75

ug/ul
ug/0.2ml/shrimp
ul/shrimp
no. of shrimp
ul
V tong (ul)
V pbs (ul)