-

Handle com or selective plates containing 50g mants using blu

e/white selection, make
sure th nsitive to or mechanical lysis cause
d by pipetting. Do not mix the cells by pipetting
To select t tent cell changes in temperature s on ice and
transform cells imm
ediately after thawing.
ransf
at of X-Gal.
Thaw compe
petent cell
s gently as
they are highly se