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  • Fluxograma Salmonella

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    Maria Dapkevicius
    6 views2 pages
    Flow diagram for Salmonella detection if foods (from a Microbiology lab class) - In Portuguese

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    Flow diagram for Salmonella detection if foods (from a Microbiology lab class) - In Portuguese

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    6 views2 pages

    Fluxograma Salmonella

    Uploaded by

    Maria Dapkevicius
    Flow diagram for Salmonella detection if foods (from a Microbiology lab class) - In Portuguese

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 2

    Fluxograma do protocolo para a pesquisa de Salmonella em alimentos

    (Baseado na norma ISO 6579)


    25 g alimento +
    225 ml gua pept.
    tamponizada

    Incubar a 37C
    (182 h)

    Transferir 1 ml para os meios de enriquecimento selectivo

    Muller-Kauffman Tetrathionate
    Novobiocin Borth (MKTN), 10 ml

    Rappaport-Vassiliadis Soy Broth


    (RVSB), 10 ml

    Incubar a 41,5 C 1 C,
    durante 24 3 h

    Incubar a 37 C 1 C, durante
    24 3 h

    Isolamento selectivo

    XLD Agar

    BG Agar

    Incubao a 37 C,
    durante 24 3 h
    5 colnias positivas
    Purificao em Agar Nutritivo

    Confirmao bioqumica

    Gram
    O/F
    TSI
    - galactosidase
    Ureia
    Indol
    MR-VP
    Lisina descarboxilase
    mobilidade

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