qRT PCR Sample Scheme

PDF/
PDF/
PDF/
PDF/
UBQ5
ORA/
UBQ5
ORA/
UBQ5
ORA/
UBQ5
ORA/
AACT1
AACT1
AACT1
AACT1
1
2
3
4
5
6
7
8
A
1-Wt Mock
10-K1 tga#2 Mock
18-Ev tga#1 ACC
31-K1 tga#1 ACC/SA
B
2-Wt Mock
11-K1 tga#3 Mock
20-K1tga#1 ACC
32-K1 tga#1 ACC/SA
C 3-tga256 Mock 12-K1 tga#3 Mock
22-K1tga#2 ACC
33-K1 tga#2 ACC/SA
D 4-tga256 Mock
13-Wt ACC
24-K1tga#3 ACC
34-K1 tga#2 ACC/SA
E 5-Ev tga#1 Mock
14-Wt ACC
26-Wt ACC/SA
35-K1 tga#3 ACC/SA
F 6-Ev tga#1 Mock
15-tga256 ACC
27-tga256 ACC/SA 36-K1 tga#3 ACC/SA
G 7-K1 tga#1 Mock 16-tga 256 ACC 29-Ev tga#1 ACC/SA
37-Wt SA
H 8-K1 tga#1 Mock 17-Ev tga#1 ACC 30-Ev tga#1 ACC/SA
38-Wt SA
No.
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24

Concentration g/l
1.1427
1.284
0.9454
0.7101
1.1313
1.5565
0.9255
0.6884
0.0032
1.3426
1.4399
1.2861
2.3856
1.2284
1.9563
0.5473
1.3607
1.5786
0.0074
1.2309
0.006
1.0109
-0.006
1.264

No.
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48

Concentration g/l
-0.0007
0.7723
1.1477
0.0983
1.5559
1.8785
0.6748
0.7579
2.1602
1.3986
1.6731
0.9581
0.8981
1.4889
1.3569
0.3512
1.1425
1.029
0.4964
0.7778
1.3473
1.1368
0.6983
0.8498

PDF/
PDF/
ORA/ UBQ5 ORA/
AACT1
AACT1
9
10
11
12
39-tga256 SA
47-K1 tga#3 SA
40-tga256 SA
48-K1 tga#3 SA
41-Ev tga#1 SA
NTC
42-Ev tga#1 SA
NTC
43-K1 tga#1 SA
44-K1 tga#1 SA
45-K1 tga#2 SA
46-K1 tga#2 SA

UBQ5

WT
0.000492129
2.15276E-05

SA
Mock

tga256
EVtga#1
0.000667067
0.002781636

Mock
ACC

K1tga#1

0.000812892

Mock

K1tga#2

0.001880269
0.003288593
0.008361232

Mock
Mock
ACC

K1tga#3

0.001352838
0.000781265

SA
SA

ACC/SA

0.087221645
0.039135935

0.000392815

SA

0.15

ACC/SA

0.000365686

0.015312856

0.008630128

SA

ACC/SA

ACC/SA

0.010842631

0.000204174

SA

ACC

0.000558849

SA

ACC/SA

0.028722667

0.000900473

SA

0.325635079

0.35

ACC/SA

0.001957136

0.011984949

0.025232331

0.014627426

SA

ACC/SA

ACC/SA

ACC

0.037883089

0.000322794

Mock
ACC

0.002635532

0.00013888

SA
Mock

0.00078442

0.019443522

0.000677825

SA

ACC/SA

ACC

0.005627998

0.002431935

SA

ACC

0.003104817

ACC/SA

0.024372119

0.1

ACC

0.00350906

0.05

Mock

Fold over reference

PDF 1.2

0.3

0.25

0.2

Wt
0.000258085

Mock

tga256
0.000421194

Mock

Evtga#1
K1tga#1

0.000154771

Mock

K1tga#2

0.000204588
0.000493073

Mock
Mock

K1tga#3

0.000296685
0.000444062

SA
SA

ACC/SA

ACC/SA

0.022624713

0.011647377

0.014456697

0.009903605

0.004645654

0.00029202

SA

ACC

0.000260185

SA

ACC/SA

ACC/SA

0.004097675

0.000212269

SA

ACC

5.04051E-05

SA

ACC/SA

0.04306657

0.045

0.007472939

0.000767256

ACC

0.014323784

0.012174449

0.015321531

0.05

ACC/SA

0.000258276

Mock

0.001985205

0.000380944

SA
Mock

0.000480864

SA

ACC/SA

ACC/SA

ACC

0.004296442

0.000474392

Mock

0.02

ACC

0.000163569

0.023640942

0.025

SA

0.000560384

0.03

ACC/SA

ACC

0.002394956

7.92272E-05

Mock

ACC

0.000223056

SA

0.004054394
0.000336561

SA

ACC/SA

0.007294479

0.00490892

0.001524376

0.015

ACC

ACC

Mock

0.005

0.003380587

0.01

Mock

Fold over reference

AACT1

0.04

0.035

Wt
0.000441042
0.000333726

Mock
Mock

tga256
Evtga#1
K1tga#1
K1tga#2
K1tga#3

0.002955103

0.000517166

0.009068291

0.00896473

0.010808666

0.020368253

0.025

ACC/SA

ACC/SA

ACC

Mock

0.000663943

0.00016401

SA
Mock

0.000171498

SA

0.003215618

0.01

ACC/SA

ACC/SA

0.002582132

0.000187487

Mock
ACC

0.000165434

0.000579355

0.00351893

0.000777034

0.000333191

0.001944035

SA

SA

ACC/SA

ACC

Mock

SA

0.000264692

0.002876947

ACC/SA
SA

0.002899239

0.000725318

0.001132735

0.000355612

0.002660715

0.000145658

ACC/SA

ACC

ACC

Mock

Mock

SA

0.000649747

0.015

ACC/SA

ACC

0.001313961

0.000321568

SA

ACC

0.000672002

0.003533404

ACC/SA
SA

0.003654583

0.002828873

ACC

ACC

0.000571872

Mock

0.000251616

0.005

Mock

Fold over reference

ORA59

0.02