PDF 1.

2 qRT PCR CT Values & FOR

Threshold Cycle (Ct) Well Threshold Cycle (Ct)
36.08
A02
31.77

Well
A01

Identifier
Wt Mock

Fold over reference

19.75617868

B01

Wt Mock

21.55

B02

29.70

0.00350906

C01

tga256 Mock

20.20

C02

7.91

5007.854

D01

tga256 Mock

19.66

D02

35.16

2.15276E-05

E01

Ev tga#1 Mock

21.02

E02

29.58

0.002635532

F01

Ev tga#1 Mock

20.68

F02

32.27

0.000322794

G01

K1 tga#1 Mock

25.58

G02

34.72

0.001772217

H01
A03
B03
C03
D03
E03
F03
G03
H03
A05
B05
C05
D05
E05
F05
G05
H05
A07
B07
C07
D07
E07
F07
G07
H07
A09
B09
C09
D09
E09
F09
G09
H09
A11
B11
C11
D11

K1 tga#1 Mock
K1 tga#2 Mock
K1 tga#3 Mock
K1 tga#3 Mock
Wt ACC
Wt ACC
tga256 ACC
tga 256 ACC
Ev tga#1 ACC
Ev tga#1 ACC
K1tga#1 ACC
K1tga#2 ACC
K1tga#3 ACC
Wt ACC/SA
tga256 ACC/SA
Ev tga#1 ACC/SA
Ev tga#1 ACC/SA
K1 tga#1 ACC/SA
K1 tga#1 ACC/SA
K1 tga#2 ACC/SA
K1 tga#2 ACC/SA
K1 tga#3 ACC/SA
K1 tga#3 ACC/SA
Wt SA
Wt SA
tga256 SA
tga256 SA
Ev tga#1 SA
Ev tga#1 SA
K1 tga#1 SA
K1 tga#1 SA
K1 tga#2 SA
K1 tga#2 SA
K1 tga#3 SA
K1 tga#3 SA
NTC
NTC

21.28
20.54
19.76
19.97
19.91
19.46
19.58
19.72
20.00
20.58
19.09
19.95
19.63
19.83
19.90
20.65
20.73
21.71
20.80
20.57
20.58
20.54
20.70
19.07
20.68
20.84
19.77
19.75
19.60
19.82
20.24
20.26
19.71
21.93
20.08
33.00
28.18

H02
A04
B04
C04
D04
E04
F04
G04
H04
A06
B06
C06
D06
E06
F06
G06
H06
A08
B08
C08
D08
E08
F08
G08
H08
A10
B10
C10
E10
F10
G10
H10
A12
B12
C12
D11
D12

31.83
30.80
28.81
28.22
25.83
24.82
27.06
30.25
24.72
26.68
27.58
26.48
26.53
28.17
25.58
25.96
27.11
23.33
25.92
27.43
26.60
24.06
25.37
27.76
31.67
31.16
32.58
28.75
29.72
30.63
32.50
31.68
31.02
31.46
30.40
32.07
31.52

0.000667067
0.000812892
0.001880269
0.003288593
0.016535843
0.024372119
0.005627998
0.000677825
0.037883089
0.014627426
0.002781636
0.010842631
0.008361232
0.003104817
0.019443522
0.025232331
0.011984949
0.325635079
0.028722667
0.008630128
0.015312856
0.087221645
0.039135935
0.002431935
0.000492129
0.00078442
0.00013888
0.001957136
0.000900473
0.000558849
0.000204174
0.000365686
0.000392815
0.001352838
0.000781265
1.898555515
0.099219449

AACT1 qRT PCR CT Values & FOR

Well

Identifier

Threshold Cycle (Ct)

Well

Threshold Cycle (Ct)

Fold over reference

A01

Wt Mock

21.33

A02

29.54

B01

Wt Mock

20.66

B02

30.02

C01

tga256 Mock

19.75

C02

33.37

D01

tga256 Mock

19.25

D02

31.17

E01

Ev tga#1 Mock

20.64

E02

31.68

F01

Ev tga#1 Mock

20.36

F02

31.57

G01

K1 tga#1 Mock

24.88

G02

33.85

H01

K1 tga#1 Mock

21.03

H02

32.95

A03

K1 tga#2 Mock

19.72

A04

32.38

B03

K1 tga#3 Mock

19.42

B04

31.68

C03

K1 tga#3 Mock

19.71

C04

30.69

D03

Wt ACC

19.12

D04

26.79

E03

Wt ACC

18.68

E04

25.78

F03

tga256 ACC

19.10

F04

27.80

G03

tga 256 ACC

19.15

G04

29.95

H03

Ev tga#1 ACC

20.01

H04

26.04

A05

Ev tga#1 ACC

20.02

A06

27.88

B05

K1tga#1 ACC

19.14

B06

29.49

C05

K1tga#2 ACC

19.18

C06

27.11

D05

K1tga#3 ACC

19.85

D06

27.60

E05

Wt ACC/SA

18.79

E06

26.74

F05

tga256 ACC/SA

19.19

F06

24.59

G05

Ev tga#1 ACC/SA

20.25

G06

26.61

H05

Ev tga#1 ACC/SA

20.55

H06

26.68

A07

K1 tga#1 ACC/SA

20.16

A08

24.70

B07

K1 tga#1 ACC/SA

20.14

B08

27.21

C07

K1 tga#2 ACC/SA

19.08

C08

25.74

D07

K1 tga#2 ACC/SA

19.78

D08

25.24

E07

K1 tga#3 ACC/SA

19.64

E08

25.75

F07

K1 tga#3 ACC/SA

20.18

F08

26.61

G07

Wt SA

18.49

G08

30.03

H07

Wt SA

19.88

H08

32.01

B09

tga256 SA

19.49

B10

32.07

C09

Ev tga#1 SA

19.87

C10

30.90

D09

Ev tga#1 SA

19.23

D10

30.58

E09

K1 tga#1 SA

19.81

E10

34.09

0.003380587
0.001524376
7.92272E-05
0.000258085
0.000474392
0.000421194
0.001985205
0.000258276
0.000154771
0.000204588
0.000493073
0.00490892
0.007294479
0.002394956
0.000560384
0.015321531
0.004296442
0.000767256
0.004097675
0.004645654
0.004054394
0.023640942
0.012174449
0.014323784
0.04306657
0.007472939
0.009903605
0.022624713
0.014456697
0.011647377
0.000336561
0.000223056
0.000163569
0.000480864
0.000380944
5.04051E-05
0.000212269
0.000260185
0.00029202
0.000296685
0.000444062
0.080407129
0.201538537

F09

K1 tga#1 SA

20.03

F10

32.23

G09

K1 tga#2 SA

19.79

G10

31.70

H09

K1 tga#2 SA

20.24

H10

31.99

A11

K1 tga#3 SA

21.05

A12

32.76

B11

K1 tga#3 SA

20.00

B12

31.14

C11

NTC

31.25

C12

34.89

D11

NTC

31.41

D12

33.72

ORA59 qRT PCR CT Values & FOR

Well

Identifier

Threshold Cycle (Ct)

Well

Threshold Cycle (Ct)

Fold Over Reference

A01

Wt Mock

19.82

A02

31.78

B01

Wt Mock

20.42

B02

31.19

0.000251616
0.000571872
0.000441042
0.000333726
0.002660715
0.000355612
0.000333191
0.000187487
0.000663943
0.000517166
0.002828873
0.003654583
0.001313961
0.000649747
0.001132735
0.000725318
0.000777034
0.002582132
0.002955103
0.003533404
0.010808666
0.002899239
0.002876947
0.005856841
0.00351893
0.003215618
0.00896473
0.009068291
0.020368253
0.000672002
0.000321568
0.000145658
0.000264692
0.001944035
0.000579355
0.000165434
0.000171498
0.00016401

C01

tga256 Mock

19.96

C02

31.10

D01

tga256 Mock

19.21

D02

30.76

E01

Ev tga#1 Mock

21.13

E02

29.68

F01

Ev tga#1 Mock

20.32

F02

31.78

H01

K1 tga#1 Mock

20.09

H02

31.64

A03

K1 tga#2 Mock

19.67

A04

32.05

B03

K1 tga#3 Mock

20.05

B04

30.61

C03

K1 tga#3 Mock

19.12

C04

30.04

D03

Wt ACC

19.22

D04

27.69

E03

Wt ACC

18.62

E04

26.72

F03

tga256 ACC

19.11

F04

28.68

G03

tga 256 ACC

19.11

G04

29.70

H03

Ev tga#1 ACC

19.91

H04

29.70

A05

Ev tga#1 ACC

19.61

A06

30.04

B05

K1tga#1 ACC

18.90

B06

29.23

C05

K1tga#2 ACC

19.36

C06

27.95

D05

K1tga#3 ACC

18.63

D06

27.03

E05

Wt ACC/SA

18.83

E06

26.97

F05

tga256 ACC/SA

19.58

F06

26.11

G05

Ev tga#1 ACC/SA

20.43

G06

28.86

H05

Ev tga#1 ACC/SA

20.49

H06

28.94

A07

K1 tga#1 ACC/SA

20.99

A08

28.41

B07

K1 tga#1 ACC/SA

19.68

B08

27.83

C07

K1 tga#2 ACC/SA

19.62

C08

27.90

D07

K1 tga#2 ACC/SA

20.81

D08

27.61

E07

K1 tga#3 ACC/SA

19.86

E08

26.65

F07

K1 tga#3 ACC/SA

20.21

F08

25.83

G07

Wt SA

19.13

G08

29.67

H07

Wt SA

19.77

H08

31.37

B09

tga256 SA

19.61

B10

32.35

C09

Ev tga#1 SA

18.74

C10

30.62

D09

Ev tga#1 SA

21.12

D10

30.13

E09

K1 tga#1 SA

18.84

E10

29.59

F09

K1 tga#1 SA

19.48

F10

32.04

G09

K1 tga#2 SA

19.17

G10

31.67

H09

K1 tga#2 SA

21.00

H10

33.57