20153373 Int J Trad Chin Med, March 2015, Vol. 37, No.

239

alzheimers disease, AD

90 SD

15 - 1-42(A1-42) AD
1
0.81.6 3.2 g/kg 0.02 mg/kg
30 dSOD

GSH-PxMDA
[(239.0548.42)s(214.3574.52)s (97.3930.69sP 0.01]
[(1.933.25)(2.273.09)(6.623.45)P 0.05]
SOD[(177.2763.10)U/mg(164.5372.58)U/mg (72.5621.04)U/mgP 0.01] GSH-Px
[(2 899.36362.27)U/g(2 407.68472.14)U/g (1 397.64442.17)U/gP 0.01]
MDA [(24.759.94)nmol/mg(27.745.82)nmol/mg (37.5617.23)nmol/mgP 0.01]

AD

Effects of Tianqi-Yizhi granules on oxidative stress in the brain tissue, and learning and
memory in Alzheimer's disease model rats Zhao Jun*, Wu Yiming, Ma Tao, Wei Dongfeng.
*

Worker Hospital of Xuanhua Steel Company, Hebei Iron & Steel Group, Zhangjiakou 075100, China

Corresponding author: Ma Tao, Dongfang Hospital Affiliated to Beijing University of Chinese Medicine,
Beijing 100078, China; Email: matao327@126.com
Abstract

Objective

To investigate the effects of Tianqi-Yizhi granules on oxidative stress in the

brain tissue, and learning and memory in Alzheimer's disease model rats. Methods A total of 90 male SD rats
were randomly divided into 6 groups by random number table method: sham operation group, model group,
huperzine A group and groups of low-, medium- and high-dose Tianqi-Yizhi granules, with 15 rats in each
group. The AD rat model was prepared by the left lateral ventricle injection of amyloid-1-42. One week after
modeling, the rats in the groups of low-, medium- and high-dose Tianqi-Yizhi granules received intragastric
administration of 0.8, 1.6 and 3.2 g/kg Tianqi-Yizhi granules, respectively; the rats in the huperzine A group
received intragastric administration of 0.02 mg/kg huperzine A solution; and the rats in the sham operation and
model groups received intragastric administration of equivalent volume of normal saline for 30 days. Learning
and memory were evaluated using the dark avoidance test. The activities of superoxide dismutase (SOD) and
glutathione peroxidase (GSH-Px), and malonaldehyde (MDA) level in the brain tissue were determined.
Results

In comparison with the model group, the latencies to step into the dark chamber in the high- and

DOI: 10.3760/cma.j.issn.1673-4246.2015.03.013
2013ZX09103002-002
81430100
075100 100078

100875
Email: matao327@126.com

240 20153373 Int J Trad Chin Med, March 2015, Vol. 37, No.3
medium-dose Tianqi-Yizhi granules groups were significantly longer (239.05 48.42 s, 214.35 74.52 s vs.
97.39 30.69 s; all P<0.01), the numbers of errors significantly decreased (1.93 3.25, 2.27 3.09 vs. 6.62
3.45; all P<0.05), the activities of SOD (177.27 63.10 U/mg, 164.53 72.58 U/mg vs. 72.5621.04 U/mg; all
P<0.01) and GSH-Px (2 899.36 362.27 U/g, 2 407.68 472.14 U/g vs. 1 397.64 442.17 U/g; all P<0.01),
and MDA level (24.75 9.94 nmol/mg, 27.74 5.82 nmol/mg vs. 37.56 17.23 nmol/mg; all P<0.01) in the
brain tissue significantly increased. Conclusion

Tianqi-Yizhi granules could attenuate oxidative stress in the

brain tissue, and improve learning and memory in AD rats.

Keywords Alzheimer disease; Learning; Memory; Oxidative stress; Rats; Tianqi-Yizhi granules

Alzheimers disease, AD

2013112020140504SOD

AD

GSH-PX

MDA

AD

20140401

2014032520140305

AD AD

AD

1.3

[1]

DBA-2

AD

5810R

Eppendorf Ultrospec

[2-3]

AD

3300 pro Amersham Biosciences

FLX800 Biotex

1.4

AD -(amyloid-, A)1-42

1 mg A1-42

AD AD

4.5 mg/ml 60 min-20

DMSO

[4]

10 min PBS 2 g/l

1
1.1

4 24 h 10%

SPF SD 4

250300 g

SCXK2009-0015SPF

0.8 mm 1.5 mm

21340

3.5 mm A1-42

5% 12 h

5 l 1 l/min

1.2

5 min

20140913
6.4 g /g

130814AA1-42

1.5

CL-05-00776Sigma-Aldrich

RIPA BCA

15

90 SD

20153373 Int J Trad Chin Med, March 2015, Vol. 37, No.3

241

A1-42 AD

3.21.60.8 g/kg

0.05 P0.01

0.02 mg/kg

30 d

0.05 1

1.6

2 h

1 5 min

(mg/kg)

(s)

()

15

276.3459.29

0.731.21

15

97.3930.69a

6.623.45a

15

0.02

225.8269.03b

1.723.90c

15

3 200

239.0548.42

1.933.25c

15

1 600

214.3574.52b

2.273.09c

15

800

194.2133.04b

5.432.32

24 h

1.7

( x s )

aP0.01bP0.01cP0.05

RIPA 4
10 000 r/min BCA

2.2

SODGSH-PX

SOD

MDA

GSH-PX MDA

1.8

SPSS13.0

P0.01

x s one-way

ANOVA Post Hoc P0.05

SODGSH-PX MDA

P0.05 P

0.01

2
2.1

30 d

P0.01

A1-42

SODMDAGSH-PX ( x s )
(mg/kg)

SOD (U/mg)

MDA(nmol/mg)

243.2647.65

72.5621.04a

37.5617.23a

146.0354.04

33.4214.05

1 647.05329.77

177.2763.10

24.75 9.94

2 899.36362.27b

164.5372.58

27.74 5.82

2 407.68472.14b

132.4969.14

35.05 9.43

2 409.39448.93b

6
6
6

0.02
3 200
1 600
800

22.63 8.86

GSH-PX (U/g)

3 572.12543.29
1 397.64442.17a

SODMDAGSH-PXaP0.01bP0.01cP0.05

242 20153373 Int J Trad Chin Med, March 2015, Vol. 37, No.3

AD

A1-42

AD

AD

AD
AD

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