MCB 3203L: Pathogenic Microbiology Lab

Midterm Study Guide

Media and Tests
Plates
5% Sheep Blood Agar (SBA)
Differential
Hemolysis patterns , ,
Whole red blood cells from sheep
Phenylethyl Alcohol Agar (PEA)
Selective for gram +
Mannitol Salt Agar (MSA)
Selective and differential
7.5-10% NaCl, mannitol, phenol red
NaCl content selects for staphylococci
Differentiates mannitol fermenters
o Yellow fermentation/acid produced
o Cerise no fermentation/no acid
Chocolate Agar (CA)
Supportive
Lysed red blood cells
o Releases nutrients from cells
Modified Thayer-Martin (MTM)