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Id Flowcharts Bacterial ID Chart
Id Flowcharts Bacterial ID Chart
GROUP 4
Description: Gram Negative, Aerobic/Microaerophilic rods and cocci
Key differences are: pigments/fluorescent, motility, growth requirements, denitrification,
morphology, and oxidase, read Genera descriptions
Examples: Acinetobacter, Pseudomonas, Beijerinckia, Acetobacter
GROUP 5
Description: Facultatively Anaerobic Gram negative rods
Key differences are: growth factors, morph., gram rxn., oxidase rxn., read Genera descriptions
Examples: Family Enterobacteriaceae and Vibrionaceae
GROUP 17
Description: Gram-Positive Cocci
Key differences are: oxygen requirements, morph., growth requirements (45C and supplements),
read Genera descriptions
Examples: Micrococcus, Staphylococcus, Streptococcus, Enterococcus, Lactococcus, Aerococcus
GROUP 18
Description: Endospore-Forming Gram positive rods and cocci
Key differences are: oxygen requirements, motility, morph, catalase
Examples: Bacillus, Clostridium
GROUP 19
Description: Regular, Nonsporlating Gram positive rods
Key differences are: morph., oxygen require, catalase
Examples: Lactobacillus, Listeria
GROUP 20
Description: Irregular, Nonsporlating Gram-positive rods
Key differences are: catalase, motility, morph., read Genera descriptions
Examples: Actinomyces, Corynebacterium, Arthrobacter, Propionibacterium
GROUP 21
Description: Weakly Gram-Positive Nonsporlating Acid Fast Slender Rods
Key differences are: acid fast, growth
Examples: Mycobacterium
Gram Positive
Cocci
Bacilli
Filamentous
Round in clusters
& tetrads:
Staphylococcus
Micrococcus
Peptococcus
Oval shape in
Chains:
Streptococcus
Peptostreptococcus
Enterococcus
Club-shaped and/or in
palisades:
Corynebacterium
Listeria
Erysipelothrix
Mycobacterium (Acid Fast)
Propionobacterium
Spore-bearing,
large, uniform:
Bacillus
Clostridium
Extensive
branching:
Actinomyces
Arachnia
Nocardia (partially
acid-fast)
Streptomyces
Gram Negative
Cocci
Neisseria
Veillonella
Bacilli
Medium-sized
cocco-bacillary:
Acinetobacter
Moraxella
Tiny-coccobacillary:
Brucella
Bordetella
Bacteroides
Pleomorphic
coccobacillary
& filamentous:
Haemophilus
Bacteroides
Pasteurella
Francisella
Actinobacillus
Eikenella
Cardiobacterium
Flavobacterium
Exaggerated
pointed ends:
Fusobacterium
nucleatum
Coiled &
sphero-plastic:
Streptobacillus
Fusobacterium
Curved,
comma-shaped:
Vibrio
Campylobacter
Spirillium
Uniformly
Bacillary:
Enterobacteriaceae
Pseudomonas
Aeromonas
Alcaligenes
Chromobacterium
Mycobacterium smegmatis
Spore Forming
+
Corynebacterium spp.
Lactobacillus spp.
Mycobacterium spp.
Bacillus spp.
Clostridium spp
Acid Fast
Strict Anaerobes
Corynebacterium spp
Lactobacillus spp.
-
+
Clostridium spp.
Bacillus spp.
Catalase
+
Corynebacterium spp.
Lactobacillus spp.
Starch Hydrolysis
Glucose Ferm. Activity
+
Corynebacterium kutsceri
Acid
Corynebacterium xerosis
Lactobacillus fermenti
Lactobacillus casei
Lactobacillus delbrueckii
Mannitol
+
Lactobacillus casei
Lactobacillus delbrueckii
Bacillus spp.
B. subtilis
B. cereus
B. megaterium
B. stearothermophilus
B. polymyxa
B. mycoides
B. macerans
B. thuringiensis
B. macquariensis
B. licheniformis
B. lentus
B. alvei
B. anthracis
B. alcalophilus
B. coagulans
B. brevis
B. circulans
B. firmus
B. pantothenticus
Starch Hydrolysis
(amylase)
+
(add creatine as catalyst
w/ reagents)
+
B. subtilis
B. cereus
B. polymyxa
B. mycoides
B. thuringiensis
B. licheniformis
B. alvei
B. anthracis
B. coagulans
(makes insecticidal
protein)
B. mycoides
B. popilliae
B. pasteurii
B. lentimorbus
B. azotoformans
B. badius
B. insolitus
B. larvae
B. laterosporus
VP
B. megaterium
B. stearothermophilus
B. macerans
B. pantothenticus
B. macquariensis
B. lentus
B. alcalophilus
B. badius
B. brevis
B. circulans
B. popilliae
Catalase
(spore is not
round, rest are)
(colony has a
rhizoidal appearance)
B. azotoformans
Swollen Cell
Swollen Cell
B. stearothermophilus
B. megaterium
(citrate pos.)
B. macerans
B. pantothenticus
B. macquariensis
B. lentus
B. badius
(citrate neg.)
B. sphaericus
B. pasteurii
B. laterosporus
(oval spore,
rest are round)
B. alcalophilus
B. brevis
B. circulans
(Motile - It is this
one )
B. larvae
(gelatinase pos.)
B. popilliae
(gelatinase neg.)
(containing spore)
B. cereus
B. insolitus
B. marinus
Nitrate Reduction
B. pasteurii
B. sphaericus
Citrate
6.5% NaCl Growth
+
B. coagulans
B. licheniformis
B. subtilis
B. insolitus
B. laterosporus
B. pasteurii
B. marinus
B. sphaericus
B. subtilis
B. polymyxa
B. lichenifomis
B. alvei
B. coagulans
Citrate
B. badius
(containing spore)
+
B. thuringiensis
B. azotoformans
B. larvae
B. pasteurii
(the width)
(non-motile)
B. marinus
B. sphaericus
B. schlegelii
B. anthracis
B. pumilus
B. polymyxa
(manitol pos.)
B. alvei
(manitol neg.)
+
B. macerans
B. macquariensis
B. alcalophilus
B. brevis
B. circulans
B. pantothenticus
B. circulans
+
B. subtilis
B. coagulans
B. licheniformis
B. macquariensis
(growth at 55C)
B. brevis
B. macerans
B. alcalophilus
B. circulans
Catalase
+
Micrococcus spp.
Staphylococcus spp.
Streptococcus spp.
(see Bergeys)
Micrococcus spp.
Staphylococcus spp.
Streptococcus spp.
Enterococcus spp.
Bile Esculin
Streptococcus pneumoniae
Streptococcus mitis
Streptococcus pyogenes Group A
Streptococcus spp. Group B
Enterococcus spp.
Streptococcus spp.
Enterococcus faecalis
Enterococcus faecium
Enterococcus hirae
+ Enterococcus mundtii
Enterococcus spp.
Streptococcus spp.
Growth w/ Tellurite
Gamma (!)
Mannitol Fermentation
Hemolysis
+
Staphylococcus aureus
Enterococcus faecalis
Enterococcus faecium
Staphylococcus spp.
Micrococcus spp.
Enterococcus hirae
(mannitol neg.)
Enterococcus mundtii
Beta (")
Yellow Pigment (Colony)
Alpha (#)
+
Micrococcus spp.
Staphylococcus spp.
Pyrrolidonearylamidase
(PYR Test)
Streptococcus pneumoniae
Streptococcus mitis
Novobiocin Sensitivity
Glucose Fermentation
+
Micrococcus varians
Yes
Staphylococcus epidermidis
- Micrococcus luteus
No
Staphylococcus saprophyticus
Optochin Sensitivity
Yes
Streptococcus pneumoniae
No
Streptococcus mitis
Enterobacteriaceae
(See Separate Flowchartt)
+ Acid
Glucose Fermentation
- Acid
Pseudomonas spp.
Aeromonas spp.
VP
Vibrio spp.
+
A. hydrophila
A. salmonicida
Luminescent
(subsp. masoucida)
P. aeruginosa
P. chlororaphis
P. cichorii
P. fluorescens
P. putida
P. syringae
(Oxidase Neg.)
A. sobria
A. veronii
+
V. fischeri
V. logei
V. orientalis
V. splendidius
Motility
-
Pigment
(Yellow Colony)
A. hydrophila
A. veronii
V. fischeri
V. logei
V. orientalis
V. splendidius
A. salmonicida
(subsp. masoucida)
P. chlororaphis
P. cichorii
P. fluorescens
P. putida
P. syringae
+
P. aeruginosa
H2S Production
Nitrate Reduction
+
A. hydrophila
30C Growth
A. veronii
37C Growth
+
V. fischeri
P. chlororaphis
+
V. logei
V. orientalis
(Colony Pigmented)
P. fluorescens
V. splendidius
P. putida
(Lecithinase Neg.)
P. syringae
(Oxidase Neg.)
P. cichorii
(Lecithinase Pos.)
Family Enterobacteriaceae
Lactose Fermentation
+
Citrobacter diversus
Citrobacter freundii
Enterobacter aerogenes
Enterobacter cloacae
Enterobacter amnigenus
Enterobacter intermedius
Erwinia carotovora
Erwinia chrysanthemi
Escherichia coli
Klebsiella oxytoca
Klebsiella pneumoniae
Serratia fonticola
Serratia rubidaea
Indole
The Rest
+
Citrobacter diversus
Escherichia coli
Erwinia chrysanthemi
Klebsiella oxytoca
MR-VP
+/+
-/+
Klebsiella pneumoniae
(subsp. pneumoniae)
Enterobacter intermedius
+/Citrobacter freundii
Serratia fonticola
Klebsiella pneumoniae
Citrate
Enterobacter aerogenes
Enterobacter cloacae
Enterobacter amnigenus
Erwinia carotovora
Serratia rubidaea
(subsp. ozaenae)
Lysine Decarboxylase
Citrobacter diversus
Escherichia coli
Enterobacter cloacae
Serratia rubidaea
Enterobacter amnigenus
Non-motile, No Pigment
(VP neg.)
Erwinia chrysanthemi
Enterobacter aerogenes
H2S Production
Klebsiella oxytoca
Motile, No pigment
??
Erwinia carotovora
+
Citrobacter freundii
Serratia fonticola
Motility pos.
Edwardsiella tarda
Morganella morganii
Proteus vulgaris
Providencia stuartii
Serratia marcescens
Serratia liquefaciens
Shigella spp. (boydii, dysenteriae, flexneri)
Shigella sonnei
Yersinia enterocolitica
Yersinia pestis
Yersinia pseudotuberculosis
Erwinia cacticida
Proteus mirabilis
Proteus penneri
Salmonella bongori
Salmonella enterica
Serratia marcescens
Serratia liquefaciens
Shigella sonnei
Yersinia pestis
Yersinia pseudotuberculosis
Indole Test
Urease
??
Please See Bergeys to ID:
Shigella spp.
Proteus mirabilis
Yersinia enterocolitica
Proteus penneri
Erwinia cacticida
Salmonella bongori
Salmonella enterica
Serratia marcescens
Serratia liquefaciens
Shigella sonnei
Yersinia pestis
H2S
Yersinia pseudotuberculosis
+
Edwardsiella tarda
Proteus vulgaris
Morganella morganii
Providencia stuartii
Motility
Erwinia cacticida
Shigella sonnei
Yersinia pestis
Urease
Salmonella bongori
Salmonella enterica
Serratia marcescens
Serratia liquefaciens
Ornithine Decarboxylase
Proteus vulgaris
Providencia stuartii
-
Edwardsiella tarda
Ornithine Decarboxylase
Morganella morganii
H2S
+
-
+
Salmonella bongori
Serratia marcescens
Salmonella enterica
Serratia liquefaciens
Red pigment
No Red pigment
Shigella sonnei
Yersinia pestis