AKAPULKO EXTRACT BY SOLVENT EXTRACTION

1. Sourcing, Collection and Authentication of Plant Material

2. Preparation of Dried Leaves for Extraction
2.1 Treatments
a. wash with tap water, air-dry to % LOD not more than 10%
b. wash with tap water, dry using dehumidifier to % LOD not more than 10%
c. wash with tap water, treat with 1.0% hydrogen peroxide solution, air-dry to % LOD
not more than 10%
d. wash with tap water, treat with 1.0% hydrogen peroxide solution, dry using
dehumidifier to % LOD not more than 10%
2.2 Ground dried leaves from treatments a, b, c, and d. Store in air tight containers
prior to extraction.
3. EXTRACTION
3.1 Aqueous Extract
3.1.1 Subject powdered leaves to series of maceration in distilled water 2:5, 2:10
and 2:15 at leaves to water ratio.
3.1.2 Decant extract at an interval of 24 hours for 5 days.
3.1.3 Evaporate to dryness the decanted extract.
3.2. Ethanol Extract
3.2.1 Subject powdered leaves to series of maceration in ethanol at 2:5, 2:10 and
2:15 leaves to ethanol ratio.
3.1.2 Decant extract at an interval of 24 hours for 5 days.
3.1.3 Evaporate to dryness the decanted extract.
4. DETERMINATION OF MIC AND MBC OF EXTRACTS
Determine the MIC and MBC values of Akapulko Leaf Extracts on Candida albicans,
Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus
epidermides, Bacillus subtilis, Aspergillus spp., microorganism obtained through
swabbing.

Preparation of Dried Leaves for Extraction

Mass of
unwashed
Leaves, g

Treatment
Treatment
Treatment
Treatment

Mass of
Powdered
Dried Leaves,
g

Yield, %

% LOD of
Powdered
Dried Leaves

A
B
C
D

AQUEOUS EXTRACT
Dried Leaves to
Water Ratio

Mass of
powdered dried
leaves, g

Volume of
Distilled Water,
mL

Remarks

Mass of
powdered dried
leaves, g

Volume of
Ethanol, mL

Remarks

2: 5
2: 10
2: 15
ETHANOL EXTRACT
Dried Leaves to
Ethanol Ratio
2: 5
2: 10
2: 15
Aqueous Extract 2:5 Ratio
Organism
C. albican
S. aureus
E. coli
P.
aeruginosa
S.
epidermides
B. subtilis
Aspergillus
spp.
Swabbing

12.5 mg

25 mg

MIC
50 mg

100 mg

200 mg