Cu 1: Trnh by vai tr cc ha cht s dng trong bi

Dung dch H2SO4 m c v HClO4 : v c ha mu

Hn hp xc tc CuSO4 : K2SO4 = 1:10 : xc tc qu trnh v c ha mu
Dung dch H3BO3 4% pH=5,5 : hp th NH3 bay ra
Dd Tashiro : ch th
Dung dch NaOH 0,1N :
HCl 0,1N : dd chun
Dung dch phenolphthalein 1%:
Giy qu: th xem NH3 bay ra ht hay cn
Cu 2: Cch x l mu nh trong bi p dng cho loi mu thc phm no?
Cch x l mu nh trong bi p dng cho tt c cc loi mu thc phm
Cu 3: Vit cc phn ng xy ra trong qu trnh tin hnh.
V c ha mu:
Protein, polypeptit, pepton
v cc hp cht cha nit

H2SO4 c, to

(NH4)2SO4 + SO2 + CO2 + H2O

Xc tc

y NH3 khi mui (NH4)2SO4 bng mt baz mnh :

(NH4)2SO4 + 2NaOH
2NH3 +2H2O + Na2SO4
nh lng NH3 bay ra bng mt acid:
2NH3 + H3BO3
(NH4)2B4O7 + 5H2O
(NH4)2B4O7 +2HCl +5H2O

4 H3BO3 + 2 NH4Cl

Cu 4: Thit lp cng thc tnh m N (mg/kg) trong mu ban u.

N

m ( protid tng )=

mD g ( NV )HCl 100
6,38
mmau

Cu 5: C th hp thu lng NH3 b phn gii bng acid khc c khng? Nu c,

trnh by cch xc nh .
C th hp thu lng NH3 b phn gii bng acid H2SO4 ( phng php gin tip).

Cch xc nh: Cho NH3 hp thu vo mt th tch chnh xc dung dch H2SO4 0,1N .
Lng d acid H2SO4 c chun bng dung dch NaOH 1 N .Khi , hm lng
nit ton phn c tnh theo cng thc:
i vi mu rn:
Nit ton phn

(V V 2) N 10 14 F 100
g
= 1
100 g
m

i vi mu lng:
Nit ton phn

(V 1V 2) N 103 14 F 100
g
=
100 g
Vm

Trong :
V1 : th tch dung dch H2SO4 0,1N cho vo bnh tam gic (ml)
V2 : th tch dung dch NaOh 1N tiu tn khi chun (ml)
N: ng lng dung dch H2SO4 0,1N
Vm: th tch mu th (ml)
m : khi lng mu th (g)
F: h s hiu chnh nng dung dch NaOH 0,1N

BI TNG TRNH:
Khi lng mu (g) :
Th tch nh mc (ml) :

Th tch mu c chun (ml) : 1 ml

Th tch HCl 0,1N tiu tn (ml): V1 = 7,6ml; V2 = 7,6ml; V3 = 7,6ml; Vtb = 7,6 ml
m N(mg/ml) trong mu ban u:
mD g N ( NV )HCl 100
m ( protid tng )=
6,38
V mau