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Question 1 (40 Marks)
Figure 1 illustrates a simplified piping and instrumentation diagram (P&ID) of a
biochemical plant for the production of specific protein X. Cells were cultivated in a
bioreactor to produce the specific protein X. The temperature of the bioreactor was
controlled through circulation of thermo-stated water into the bioregctor jacketed layer
ayhere the pHi of the bioreactor wa8 conirolled viz base addition: Temperature changes were
mainly due 10 heat of reaction. The dissolved oaygen level was maintained above 40%
uration above through gas control loop. Oxygen transfer was enhanced via vigorous
mixing scheme. Due to a high oxygen demand by cells, a high gas (i.e. air) flow rate was
set before being fed as o:
ren supply. Since foaming was expected during the fermentation
process in the bioreactor, antifoam was used to maintain the (desired liquid level inside the
bioreactor: The specific protein was qeqgvéred at purity of 99% efter underwent the
downstream processing phase. In the sedimentation tank, the biomass was discharged
whilst the supematant was fed to the chromatography Column for purification stage. The
entire plant was fully automated and the process control studies aimed at developing and
designing cfficient and safe plant operation. All together, there are eight feedback control
loops were installed for the plant, Identify all the feedback control loops and complete
Table 1
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Figure 1 Simplified piping and instrumentation diagram of « biochemical plant.
Universi Teknatog Malaysia Coarre: SKF 3143 Prucess Control & Dynamies 201272013 Seon 11
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