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Page Question 1 (40 Marks) Figure 1 illustrates a simplified piping and instrumentation diagram (P&ID) of a biochemical plant for the production of specific protein X. Cells were cultivated in a bioreactor to produce the specific protein X. The temperature of the bioreactor was controlled through circulation of thermo-stated water into the bioregctor jacketed layer ayhere the pHi of the bioreactor wa8 conirolled viz base addition: Temperature changes were mainly due 10 heat of reaction. The dissolved oaygen level was maintained above 40% uration above through gas control loop. Oxygen transfer was enhanced via vigorous mixing scheme. Due to a high oxygen demand by cells, a high gas (i.e. air) flow rate was set before being fed as o: ren supply. Since foaming was expected during the fermentation process in the bioreactor, antifoam was used to maintain the (desired liquid level inside the bioreactor: The specific protein was qeqgvéred at purity of 99% efter underwent the downstream processing phase. In the sedimentation tank, the biomass was discharged whilst the supematant was fed to the chromatography Column for purification stage. The entire plant was fully automated and the process control studies aimed at developing and designing cfficient and safe plant operation. All together, there are eight feedback control loops were installed for the plant, Identify all the feedback control loops and complete Table 1 OUalversil Tekaolog! Malaysia Cours SKF 5143 Process Control & Dynamics 20122013 Sem It DO NOT WRITE ON THE BACK OF THISSHEET: USE ONE SIDEONLY INSTRUCTIONS: Esch paze ofthis ra must be signed by the suden. Heese complet all infernstion nected Nan: ICNo. @UIM OF i @X ees « \ Oo ——_ xe @ ths Jans sor tukontaroc EOLUNN. aceon! sancny {e+ — cots Lore e-e Cte puny pier GH) 8H marie Dee ott 3 leone GG) nea rae C_ Seb BENTATION toi Tiomase-dcarne Flow come Fo tana: Figure 1 Simplified piping and instrumentation diagram of « biochemical plant. Universi Teknatog Malaysia Coarre: SKF 3143 Prucess Control & Dynamies 201272013 Seon 11 DO NOT WRITE ON THE BACK OF THIS SHEET: USE ONE SIDE ONLY INSTRUCTIONS: Ean pegeortns erat mus be signed by the suden,Fesse complete information nese Name ICNo.

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