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  • Hexokinase Enzyme Structure and Reaction

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    Hexokinase is the enzyme EC 2.7.1.1, which catalyzes the first step in glucose metabolism by phosphorylating glucose to glucose-6-phosphate using ATP. Hexokinase reactions have been found in all organisms. It has a molecular weight between 50-100 kD and works best at a pH of 7.5-9.0. Hexokinase requires magnesium as an activator and is inhibited by glucose-6-phosphate. There are four types of hexokinase enzymes. Hexokinase is isolated from samples using Triton X-100, Percoll/NaCl separation, and analyzed using nanoLC/ESI/MS/MS to determine purity.

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    HEXOKINASE (2)

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    Hexokinase is the enzyme EC 2.7.1.1, which catalyzes the first step in glucose metabolism by phosphorylating glucose to glucose-6-phosphate using ATP. Hexokinase reactions have been found in all organisms. It has a molecular weight between 50-100 kD and works best at a pH of 7.5-9.0. Hexokinase requires magnesium as an activator and is inhibited by glucose-6-phosphate. There are four types of hexokinase enzymes. Hexokinase is isolated from samples using Triton X-100, Percoll/NaCl separation, and analyzed using nanoLC/ESI/MS/MS to determine purity.

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    8 views11 pages

    Hexokinase Enzyme Structure and Reaction

    Uploaded by

    desyabilla
    Hexokinase is the enzyme EC 2.7.1.1, which catalyzes the first step in glucose metabolism by phosphorylating glucose to glucose-6-phosphate using ATP. Hexokinase reactions have been found in all organisms. It has a molecular weight between 50-100 kD and works best at a pH of 7.5-9.0. Hexokinase requires magnesium as an activator and is inhibited by glucose-6-phosphate. There are four types of hexokinase enzymes. Hexokinase is isolated from samples using Triton X-100, Percoll/NaCl separation, and analyzed using nanoLC/ESI/MS/MS to determine purity.

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    of 11

    HEXOKINASE

    Korry Novitriani
    20510045

    Pendahuluan
    Heksokinase

    EC. 2.7.1.1
    (2) menunjukkan nama kelas
    (transferase),
    (7 ) subkelas (fosfotransferase) dan
    (1) sub-sub kelas (fosfotransferase
    dengan gugus hidroksil sebagai
    penerima),
    (1) D-glukosa sebagai penerima
    gugus fosfat.

    Hexokinase
    Reaksi

    hexokinase telah
    ditemukan di setiap organisme
    Glukosa
    ATP

    Reaksi Heksokinase

    Karakteristik Hexokinase
    Ukuran

    : 50 kD - 100 kD
    pH Optimum : 7,5 - 9,0
    Titik isoelektrik: PI, 5,25 dan P-II,
    4,93
    Stabilitas: suspensi kristal yang
    stabil selama 6-12 bulan 2-8 C.
    Aktivator : Mg2+
    Inhibitor : Glukosa-6-Phospat

    Jenis Heksokinase
    Heksokinase

    I
    Heksokinase II
    Heksokinase III
    Heksokinase IV

    Struktur Heksokinase

    Preparasi Sampel

    Isolasi Heksokinase
    Sampel

    Triton X-100

    Percoll/ NaCl

    NanoLC/ESI/MS/M
    S

    Kemurnian

    Daftar Pustaka

    Toshihiko Aki and Shuichi Yanagisawa. Application of


    Rice Nuclear Proteome Analysis to the Identification of
    Evolutionarily Conserved and Glucose-Responsive
    Nuclear Proteins. Department of Applied Biological
    Chemistry, The University of Tokyo, Japan, and Core
    Research for Evolutional Science and Technology
    (CREST), Japan Science and Technology Agency (JST),
    Kawaguchi, Saitama,Japan. 2009

    Mioara Larion and Brian G. Miller. Global Fit Analysis


    of Glucose Binding Curves Reveals a Minimal Model
    for Kinetic Cooperativity in Human Glucokinase.
    Department of Chemistry and Biochemistry, The
    Florida State University, Tallahassee, Florida. 2010

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