EXPERIMENTAL STUDY ON DISINFECTION

PERFORMANCE OF A KIND OF QUICK-DRYING

ANTISEPTIC SOLUTION
SU Yu-xin,JIANG Li,WU Xue-bing,REN Zhe,TIE Jin-feng,ZHANG Wen-fu(Institute of
Disease Prevention and Control,Academy of Military Medical Sciences,Beijing
100071,China)
Objective To study the disinfection performance and influential factors of a kind of quick-drying liquid
skin antiseptic with chlorhexidine acetate as the active principle.Methods Suspension quantitative
germicidal test and animal toxicity test were used to carry out laboratory observation of this
antiseptic.Results At room temperature,the killing rate of Staphylococcus aureus,Escherichia
coli,Pseudomonas aeruginosa and Candida albicans exposed to the 10-fold dilution of compound
chlorhexidine alcohol antiseptic solution containing chlorhexidine acetate 8 mg/L and 70%(v/v) ethanol for
2 min were 100%.Adding BSA to the bacterial suspension slightly influenced the germicidal efficacy of the
antiseptic solution in killing E.coli.With the increase of pH and temperature,the germicidal efficacy of the
8-fold dilution of the antiseptic solution could obviously be enhanced in killing E.coli.The acute oral toxicity
test of the disinfection solution showed that LD50 values in both rats were 5 000 mg/kgbw,which were
practically non-toxic.The skin irritation index of stock solution on rabbits was 0.The mouse bone marrow
micronucleus test showed no non-nuclear-induced effect.Conclusion The quick-drying antiseptic has rapid
germicidal action on vegetative forms of bacteria and natural bacteria on skin,no action of inducing
micronucleus,no irritation to skin,and belongs to non-toxic substance in animal test.
Key Words chlorhexidine acetate ethanol compound antiseptic bactericidal effect toxicity

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