DAFTAR ISI

DAFTAR ISI........................................................................................................iii
DAFTAR GAMBAR............................................................................................v
DAFTAR LAMPIRAN........................................................................................vi
I.

PENDAHULUAN..................................................................................1

II. TINJAUAN PUSTAKA.........................................................................4

2.1 Tinjauan Botani Voacanga foetida (Bl). K.Schum...........................4
2.1.1 Klasifikasi Tumbuhan............................................................4
2.1.2 Nama Daerah.........................................................................4
2.1.3 Morfologi...............................................................................5
2.1.4 Kandungan ............................................................................5
2.1.5 Khasiat....................................................................................6
2.2 Sel.........................................................................7
2.2.1 Siklus Sel................................................................................7
2.2.2 Apoptosis................................................................................9
2.3 Kanker..............................................................................................9
2.4 Kanker Leukimia..............................................................................10
2.4.1 Penyebab Kanker Leukimia....................................................11
2.4.2 Karsinogen..............................................................................12
2.5 Obat Antikanker................................................................................14
2.6 Sitotoksik..........................................................................................16
III. PELAKSANAAN PENELITIAN.........................................................16
3.1 Waktu dan Tempat Penelitian...........................................................16
3.2 Metodologi Penelitian......................................................................16
3.2.1 Alat dan Bahan.......................................................................16
3.2.2 Rancangan Penelitian..............................................................17
3.3 Prosedur Penelitian...........................................................................17
3.3.1 Pengambilan Sampel..............................................................17
3.3.2 Ekstraksi Sampel....................................................................18
3.3.3 Fraksinasi Sampel ..................................................................18

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3.3.4 Uji Kandungan Kimia.............................................................19

3.3.7 Pemeriksaan Aktivitas Antikanker.22
3.3.7.1 Pembuatan Reagen Uji Sitotoksitas .........................22
3.3.7.2 Persiapan Larutan Uji ..............................................23
3.3.7.3 Pembiakan Sel Leukemia L1210 .............................24
3.3.7.4 Uji Daya Hambat Terhadap Pertumbuhan ...............25
Sel Leukemia L1210 secara in vitro
3.3.8 Analisa Data............................................................................26
DAFTAR PUSTAKA...........................................................................................28
LAMPIRAN.........................................................................................................31

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DAFTAR GAMBAR

Gambar

Halaman

1. Struktur Senyawa Hasil Isolasi daun Voacanga foetida..................................6

DAFTAR LAMPIRAN

Lampiran

Halaman

1. Gambar Daun Voacanga foetida (Bl.) K.Schum...31

2. Skema Kerja Ekstraksi dan Fraksinasi...........................................................32
3. Skema Kerja Pemisahan Senyawa Hasil Isolasi ...........................................33
4. Prosedur Kerja Aktivitas Antikanker.............................................................34
5. Skema Kerja Uji Daya Hambat Pertumbuhan Sel Leukimia L1210.............35
6. Pemetaan Pengisian Larutan Uji pada Plat Kultur Sel..................................36

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