Bai 4: XAC INH

PHOSPHAT, PHOSPHO
TNG TRONG NC
Nhom 4 12KMT
Trinh bay: Ng Quang Tri
GVHD: TS. T Thi Hin &
ThS.Nguyn Ly Sy Phu

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NI DUNG
S lc v phospho
Nguyn tc xac inh
Cac yu t anh hng
Dung cu hoa cht
Cach tin hanh

1. S lc v phospho
Dang tn tai:
Orthophosphate (PO43-, HPO42-, H2PO4-),
polyphosphate, phosphat hu c

Ngun phat sinh phospho trong nc:

- Thm thoat t phn bon d tha
- Cht thai ngi va ng vt
- Cac hoa cht ty ra va lam sach

1. S lc v phospho
Anh hng cua phospho
trong nc:
- Ham lng phospho cao tao phat trin
manh khi tao cht, xay ra qua trinh ky khi
giam lng oxy trong nc
mt trong nhng nguyn nhn gy nhim
mi trng nc
- Ngoai ra, phospho nng cao anh hng
xu n con ngi, gy mt s bnh v huyt
ap, h hp,..

2. Nguyn tc xac inh

Xac inh phosphat (orthophosphat)
Trong mi trng axit,

Orthophosphate + dd ammonium molybdate

(NH4)6Mo7O24.4H2O + dd potassium antimonyl
tartrate K(SbO)C4H4O6.1/2 H2O phc
antimony phosphomolydate + acid ascorbic
phc molybden mau xanh

o hp thu quang tai bc song 880nm.

Xac inh phospho tng

Ta v c hoa cac dang phospho v
orthophosphate, sau o lam tng t nh
phng phap xac inh phosphat.

3. Cac yu t anh hng

- Arsenate anh hng nng 0.1 mg

As/L vi cung tao mau xanh vi molybden
- Silicate khng gy anh hng nng
1-10mg/L.

4. Dung cu hoa cht

Xem tai liu thc hanh quan trc

trang 20-21

5. Cach tin hanh

1. Ly mu va bao quan
Chai thuy tinh hoc nha PE, PVC nu ham
lng phospho thp thi phai dung chai thuy tinh
Bao quan mu nhit < 40C

2. Phn tich mu
a) Xac inh phosphat
Erlen 125mL: Mu (50ml) + phenolphthalein
(1 giot) + acid (n khi dd mt mau) + thuc
th (4mL)
o quang bc song 880 nm sau 10 phut
va trc 30 phut

5. Cach tin hanh

b) Xac inh phospho tng
- Erlen 125mL: Mu (50ml) + phenolphthalein (1
giot) + acid (n khi dd mt mau) + 1mL acid
manh
- Sau o cho 0.5g K2S2O8 hoc (NH4)2S2O8
un dd con 10ml ( chuyn phospho v
orthophosphate) + nc ct (20ml) +
phenolphthalein + NaOH 2M dd mau hng nhat
- Chuyn toan b vao binh 50mL ri thm
4ml thuc th va o quang nh bc xac
inh nng phosphat.

5. Cach tin hanh

3. Dng ng chun
- Rut ln lt 0.0; 0.5; 1.0; 2.0; 3.0; 4.0; 5.0 mL
dd P 10mg/L cho vao 7 binh inh mc 50 mL
- Thm 4mL hh thuc th va inh mc thanh
50mL bng nc ct
- o mau bc song 880nm

4. Tinh toan kt qua

T hp thu A, ta da vao thi ng
chun tinh nng phosphat va phospho
tng trong mu.

Danh sach thanh vin trong nhom

1. Ng Quang Tri
1217280
2. Pham Thi Bich Trang
1217268
3. Trn Thanh Tung
1217303
4. Nguyn Vu Thinh
1217241
5. Nguyn Thi Thu Hin
1217080
6. Trn Nguyn Thin Trang 1217270
7. Nguyn Thi Tu Trinh
1217278
8. Nguyn Thi Thanh Vy
1217321
9. Trn Thi Thng Th
1217257
10. Nguyn Thi Thanh Tuyn 1217295

Cam n thy c va cac ban

a lng nghe !!!
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