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HighdisseminationofextendedspectrumlactamaseproducingEnter...PubMedNCBI

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WaterRes.2014Jun156:3747.doi:10.1016/j.watres.2014.02.041.Epub2014Mar6.

Highdisseminationofextendedspectrumlactamaseproducing
Enterobacteriaceaeineffluentsfromwastewatertreatmentplants.
OjerUsozE1,GonzlezD2,GarcaJalnI2,VitasAI2.

Authorinformation
1

DepartmentofMicrobiologyandParasitology,MicrobiologicalFoodandWaterLaboratory,
C.I.F.A.,UniversityofNavarra,C/Irunlarrea1,31008Pamplona,Spain.Electronicaddress:
eojeruso@alumni.unav.es.
2
DepartmentofMicrobiologyandParasitology,MicrobiologicalFoodandWaterLaboratory,
C.I.F.A.,UniversityofNavarra,C/Irunlarrea1,31008Pamplona,Spain.

Abstract
Waterenvironmentsplayanimportantroleinthedisseminationofantibioticresistantbacteria
amonghumans,animalsandagriculturalsources.Inordertoassessthespreadofextended
spectrumlactamase(ESBL)producingEnterobacteriaceae,weanalyzed279effluent
samplesfrom21wastewatertreatmentplantsinNavarra(northernSpain).Atotalof185
cefpodoximeresistantbacteriawereisolatedonChromIDESBLagarplates,withhigh
predominanceofEscherichiacoliamongisolatedspecies(73%).ESBLproductionwas
determinedbydifferentmethods,concludingitspresencein86.5%oftheisolatesbythe
combinationdisktest,75.7%bydoubledisksynergytestand73.5%byMicroScan()NM37
automatedsystem.PCRandsequencinganalysisshowedthatthepredominantlactamases
(bla)geneswereblaCTxM(67.4%)followedbyblaTEM(47%),blaSHV(17.4%)andblaOxA
(8.3%)furthermore,twoormorelactamasesgeneswerefoundin34.9%oftheisolates.The
resultsdemonstratethehighprevalenceofESBLproducingEnterobacteriaceaeineffluentwater
fromwastewatertreatmentplantsandconfirmtheneedtooptimizecurrentdisinfection
proceduresandtoimprovemanagementofwastewaterinanefforttominimizereservoirsof
resistantbacteria.Furtherstudiesareneededforexaminingthepresenceofthesebacteriain
otherenvironmentsandfordeterminingthepotentialdisseminationroutesoftheseresistances
aswellastheirimpactonhumanhealth.
Copyright2014ElsevierLtd.Allrightsreserved.
KEYWORDS: ESBLEnterobacteriaceaeExtendedspectrumbetalactamaseResistance
Wastewatertreatmentplant
PMID:24651016[PubMedindexedforMEDLINE]

http://www.ncbi.nlm.nih.gov/pubmed/24651016

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HighdisseminationofextendedspectrumlactamaseproducingEnter...PubMedNCBI

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