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12 Daohungcuong Lexuanvan
12 Daohungcuong Lexuanvan
2011
1. M u
Cy da cn cn gi l trng xun, hoa hi ng, bng da. Tn khoa hc
Catharanthus roseus L. G. Don, thuc h Trc o Apocynaceae. Cy nh, cao 0,4 0,8m,
c b r rt pht trin, thn g pha gc, mm phn trn, mc thnh bi dy, cnh thng
ng. L mc i, thun di, u l hi nhn, pha cung hp nhn, di 3 - 8cm, rng 1 2,5cm. Hoa trng hoc hng, mi thm, nm cc k l pha trn, di 2 - 4cm, rng 2 3mm. Da cn pht trin tt v c quanh nm Bnh nh [1].
Vincristine (vcr)
Vinblastine (vlb)
Sau khi chy ph HPLC (hnh 3), chng ti kt hp vi khi ph lin hp (MS)
xc nh thnh phn ha hc cc cht c trong dch chit ca r cy da cn hoa
hng. Kt qu c th hin hnh 4 v hnh 5.
Trn sc k hnh 4 v 5, sau khi qua h thng LC/MS, trong khong khi
lng 1001000, vi thi gian lu (RT) t 925,01; xc nh c 2 cht
(Vinblastine v Vincristine) vi thi gian lu l 10,62; 10,82 pht (hnh 4). Ngoi ra, ti
2 peak vi thi gian lu l 10,09 v 10,29 pht, mi peak ny c hai cu t ging nhau
v cng thc phn t nhng khc nhau v cu to (hnh 5). Bng phn mm
Chemsketch, chng ti phn tch m/z cho tng peak c th nh sau:
* Kt qu phn tch ti thi gian lu 10,09 pht.
Trn sc k hnh 5, tng ng vi peak c m/z = 353,4 cn c cc peak
tng i ln c khi lng 321,5; 222,3; 144,3. Trong peak 353,4 chnh l ion phn
t hay peak gc [M+] c nhn din l ca mt trong hai cht Rhazimol v Ajmalicine.
Kt qu s phn mnh c th hin hnh 6 v 7.
(1)
H 2C
(2)
N
N
O
(1)
N
(2)
H
(1)
O
N
H
Ajmalicine
Formula Weight
H
CH
Formula Weight
[B]
[A]
HC
= 321.3923914 O
H
N
= 352.42686
HN
CH
Formula Weight
(2)
3C
+
2
2+
Formula Weight
[A
++
= 222.2839628
H
+
= 144.1925514
88
CH
+
2
HOH2C
HOH2C (3) CO2CH3
(4)
(3)
Formula Weight
N
(4)
CH2
(4)
(3)
Formula Weight
CH3
Rhazimol (deacetylakuammiline)
Formula Weight
= 352.42686
= 280.3642
= 238.32752
Peak 354,4 nm bn cnh peak gc, ng thi c khi lng bng [M+ 1]+
chnh l ion ng v ca [M+]. Cc speak ng lu nht l 321,5 v 144,3, chnh l
cc ion mnh ch yu do s bn ph ca ion phn t c my ghi li.
Da vo cng thc cu to ca 2 cht cng vi s h tr ca phn mm
Chemsketch, chng ti cho rng c ch phn mnh nh hnh 6 v 7.
Vi ph khi hnh 5 v s phn mnh hnh 6 v 7, cho thy s phn mnh
m/z 352,4 c th theo 4 hng:
+ Hng (1): [M]+ (352,42686) [A]+ (321,3923914)
[A]+ (321,3923914) [A1]++ (222,2839628)
+ Hng (2): [M]+ (352,42686)
[B]+ (144,1925514)
89
O
(7)
HN
CH3
O=C
(1)
H
N
O
O
(1)
H 3C
Formula Weight[A]
CH3
CH
Alstonine
+
Formula Weight [M] = 349.4024914
O
+
(2)
C
H
N
O
N
= 317.3606314
[A1]+
= 335.3759114
Formula Weight
CH3
H 2C
= 295.3540142
O
N
H 2C
Formula Weight
= 289.3505314
+
[A2]
(3)
CH3
(3)
(4)
O
(4)
= 277.3398314
(3)
O
NH
Serpentine
Formula Weight
H 3C
= 349.4024914
N
H
Formula Weight [D]
CH3
+
= 235.3031514
(5)
N
N
H
Formula Weight[E]
= 223.2924514
N
H
+
Formula Weight [D 1 ] = 220.2686314
Peak 350,4 nm bn cnh peak gc, ng thi c khi lng bng [M+ 1]+
chnh l ion ng v ca [M+]. Cc speak ng lu nht l 335,4; 317,4 v 289,3;
chnh l cc ion mnh ch yu do s bn ph ca ion phn t c my ghi li.
Da vo cng thc cu to ca 2 cht cng vi s h tr ca phn mm
Chemsketch, chng ti cho rng c ch phn mnh nh hnh 8 v 9.
Vi ph khi hnh 5 v s phn mnh hnh 8 v 9, cho thy s phn mnh
m/z 349,5 ca Serpentine v Alstonine nh sau:
+ Hng (1): [M+] (349,4024914)
[A]+ (335,3759114)
[A]+ (335,3759114)
[A1]+ (317,3606314)
[B]+ (295,3540142)
Cc hng (3), (4) v (5) mc d cng c s phn mnh nhng cc ion phn
mnh li khng nm trong cc peak ca ph LC/MS.
Da vo nhng lp lun cng nh kt qu nhn c trn ph , chng ti
xc nh s xut hin ca cc mnh ion c m/z 335,4; 317,4; 289,3 l do s ph v
[M]+, cn ion m/z 350,4 chnh l ng v [M+1]+ ca [M]+ m/z 349,5. Nh vy hp cht
ti thi gian lu 10,29 pht chnh l Alstonine [5].
Nh vy, cc thi gian lu 10,09 v 10,29 pht xc nh c 2 alkaloid l
Ajmalicine v Alstonine, iu ny ph hp vi cc ti liu tham kho [2] [5].
T nhng kt qu xc lp, chng ti xc nh c trong dch chit ca r cy
da cn hoa hng c 4 alkaloid (vinbalstine, vincistine, ajmalicine v alstonine) [5],
trong c hai alkaloid quan trng l vinbalstine v vincistine. Ta tnh c hm lng
ca 2 alkaloid theo total ion:
%vlb =
311703752
x100%
584909354
= 53,29% ; %vcr =
73396665
x100%
584909354
= 12,55%
92