ecaloia microarray Identificazione di mutazioni e polimorfismi mediante tecnologia microarray ‘Seti Frascon Sara Beeb? Lovina Ros, Bei Gis? "na Opera Gna Chogentie Aenda Oxpedir Caegs Pence: *Dipariment Arca Cros Medio Chiari, Universi’ a Fene Plrece “aro d Rfrneno Regionale Genco ePsigenomice perl Sila e Aplcaone dele Teno a Ala Iimovcione in Ambit Biomed, Regione Toscana, Aco Onoda Cares, Frese ‘TECNOLOGIE MICROARRAY DISPONIBILL cnrgrenert sora ate poe eng] Seem oes secvemeattoeaee beets Sra artepetientenremede Schaerer Sobre aeeten aetna ieeeeret saree teen Eee ieeretmce ere pot ceeee amram Shee leet neces Tomer eer oer atgeemeraes inkieeer emo Seeortaemsane Euiiecc cece eoahreeermatemcas cheubroesauoenae eee SO sonaneree ers gmmercemeninceee fecermmeateennceceeiaas fee Seccroe ieee seer ees ee leceerttaceyucitatnts Seeactsren manta Seugrencmlaontectitante Ssh eterna mate semaine Sas aed ra mae eranns Sepvacpeet ee Sega ote en ar Coacar arene Shetek Sena es tinea Gece cisamecoanc, ieee anitrvct ost Soc acececaayttens Since rorenenreerert ao Seccngieetncanios ota? 1 soqencao seeing matzo sug sala. Uo Seton fro 8 ponte quo pa za cata mente nutipHONA goramco. Tecnologie mleroaray basatesulbridazione passive La spect go torino acne ulate pr ‘eign msc pelos una sing bes rghit aymaphans SN) be fate co'atareereure dl mating torpor ala ae 10% a molec DNA ruta asrgl lea) Gogh ni DRAONA 0 DNAANA con porta crponenanaa Inte), oregon aust cha erecora negli he ‘on neo peta completa (misma). ime qua eniogn rll su oma Dae nok oe probiamanchedesote eg. *)evatoana es reorranoneloitvo diragp 9p fo condor svmpencaappoprate (srgenea {hoa e mea, Ux singers cove dai ao pore. {or al bs prtamentn compen, 8 renaer laa sl sip dake sonst sonar trent dala soperingofary dle el ce ron {ie patente conploneta. ta modo | sera hee var susray sno cou slo apt Brecon ‘ott comperereaet, Appeam ua gee chin [Expropratm aga lee saan sine acon {razon avr atararet geo mecle Iago mado aspectic La snorsa oma scots ‘Staogunge eoptac aenay un orport che oo ‘Stans dctmnazione ral reprotarere comp ‘neta 9 ron poretiamert conpemanat fair ‘zt da vrs puto ing. Appar pomato {drt come agpunginrio dle ror tial Steg nn wes crt pre 2) Lanan vera dt agnodeltip utara gt au cost porta clzzazore Netter igh ape oa ebustzza tal dele aches basse sulerdazone, sno Sa Propose tsa tata una set tapos in raga mental flere naa empertira a mat ve [ta poretorense canplonorat © on poetanors ‘erploneian Luse on evan ara (THA) ‘enorpornoarens con a modrazone cima dru ‘loots Att cooeetidna, 0) nd Seah i Inatquosorotnsera,cnsoreetraggungenrta ara magpie stabi tama dl OMA Ina so metig Iestonchetritean AT oC pa ba) an (rasp mica npendore lr cote baaLa tecaologia mioaray * Lalor apples outer nog tut dmuazione cen wcndnla mctoarayo par ancra ca mos Prep dbdazere dca anche wz a mato femora (ordasone wlonont areal medal do rain) austutlzare sequorzs "taf eves ace Sate "brcoa? 026, acct door seats Berle mataacn/SAPs. Quote sequanza tag ighorro sabi a atone aay, reaenge i aipgrase ‘Seuguatret sepa specter apateo dain spr ‘Spans fennductiespreseaia PCA Voces Uinsoreie spat auet ana mead na pare (ach dacs nto basa sa aez0 oir Un Store supe el etd beats bacon pase ‘sap unto concomo serait al agra co ty, 5 per righoneert al process aon, eit con supp dls oencaga dl soar etn baa au aeze de cap cote Coens Nanogen ¢ dota Bison tn Tal erlona, ee ‘Sa angen rca © chm tara cha Gs ‘etwni La dosarisone quota ead vera ato ‘ial aucesev poral Depa questa proms, ocazram Fatnne si eta starts basa caltordaone pais, et sono esr satcamert san taonce ene ano I quadagno do sega caso (gah of pret sina eee ce vlan a pra eat ‘tai bazar (ons ot Pyaseton str pi taza olgenussch corplemontar ad uncon name- "0 varacen doa sequenca & iaesso © murano, ‘pata un eampone dormer, I guacapre dts gil dibndacone cash alcaso cit carpe Setar i matt. Gueeto meta onset cae: ‘ezagone @ maasioniShPe ot ea Tensions ‘mascot Ps nen nt, scons azo sigonases ‘ compementr ta equa bapa vaao a pata elotvedl gras ditscaina ch sha rl aso owen aealzzare un vos pop een et Sonia SP, snes pre’ pane irc dle ‘vo varara ganache, che dr essere aa sro. Gunso ce sonal a itidcione Toco ce vata uadgra dl sega ‘ona pus sess wszao per str tte le psa ‘rain al soqueaai nto DNA fs nn et) (he pr caraotzare le mario gt SNPs ok Vins ‘Stictzata ura opeadalgonssaob ane od eon 4) po opt paseo mutazora ua dtm oe ‘one de ge. Ops eigonasoos,sltarante nde 2025 bes, pesos avararie nposzane cass) ‘edo cine amigos spect cata eazore (bigacon. lta Fgura apr Ioogn 3 nats zea per fara amie medians {eqn segnae brdazone* Goes comprendan, ‘Holgonacleots per esa tute poseb oxen! tin ipo nue (24%, dove We trate bas ‘ata soqunca ce onde Suda) gone ‘erento prosrgac ever astzon 2x4", {Gwe x8 ln rancecza det sera) lelponacece pet {ec lapresnaad detenon luna crt mast EN dove 81 numer ch lero orc), ‘acnarscomprorsbie cme, con esta metosege, 8 ‘esc sero pk ne aba rn isle nents otemabie: i prods aud le daar ‘ample marca @ ala canon. n pesaca deg ok ‘enclst spt por all mut, a carn are ‘api eae wise nn avers sn game ‘is eaten non sa sta segs ures a2 mado chvaldala sera del sgrae eavione taza un aay a lgonatoots carlomerar la ‘enc wipe. Lavra sequen ovo vali ean a rane ala per hen etme nconsponden solemn otponaseoks Sh rooede qu she Beacons dt campers mare © fia seanacee, Una mene onecigale fasta tne amelta persa de ogale aso a elo dog ‘sigenaseeta spect! pg all real lost ‘oegt la mulszone Geena we pera det seal (it 0% goto al sognde cele h wn cargane tesmors (Pg). Con acto metode unary degra pr vere, alain tamer N pai bt, et "arazor dl seqvoea pol conse 2M algonses a Untre dfpprocco pod de sega Se Snes utnatinara seen anaes ears man. (Cvavnone at gsmgrodanpaie eoreama gas ‘Stunaon aera as aca ce as Monro Retest | Pe eR mnt ow ‘Seow pancannucncee maaanLa tenon meray Hence onsite pmo espe ale noma ua tsp: m prose muah pea et Sop otaone stag str pt en See ees ee x Sentara a ater eters arene Sone rrstermaraceteern sismesasowmae eet Sone oe eeirent ae Sei Sire icmsigt pear nr ee enterecetnts steee ngs spews seca pogo ae ssa tees Leese Sone SoS cn pon arais seas mnceraecie Soetectieceeamemrteareea enieeta eae ona Sonne eee stand ae aca Saree snattonampans Sesetgntm erate once ignitor nat seater ior anewen Smeedatytapncnyetoe mracnaameatye Sontag See aten ayetose se te rs ante Eeeccetedrpetenreeet ies satscar So ieee Bias ts Sa gn eo eae fanaa Ss rece mapa ea aon 9 Someta s2 entazor (6) endzone. Weainete nin carne ‘torte eee) un erat Sl segrie © ‘ison (qusapne det soya) ra ponzane dato. ‘icles pester carolontre als eeu ‘an 2) ora po del 50% al sograle rapa a ‘anti pra sagan corspanoea deg Sercleats spect! por a sequrea wip casa (ila mancarze a portetaconplerenafai feat 8 Coil compen cores: Scare, et ‘uta gossano nen tapoccha uel doe stazo. ‘6 coate quad soe qusso prime mada Ia resorea dl egorulata rata eappeasione ‘iragrtneconplseso per remorareleersbi ot ‘octet arse? Ltzo cient onda 1h parmaseo un ererent a apoto sega ‘Sion una mgr certo neta quanozone gale guna uso da oto toes sone I ‘Sogo spermartale esse a prod Hac» colar ‘orth eeena pnts dale 1 mon Frosort a canpin eam, Lamina non ot ‘ain un nsrsone na ang a rune seen Riteimenns GP) RY Cumpione : «a | int itt rt pe cereecrrance ‘Sriram mado 9 ested Ta === ron Beemer omesta, Le costo nilotc sono sist ers tite cratorzzto nun, gonomirate, rodcono tne porta sepale&bsasone ps apart dle ootie neeton'® diesen. Os suas suo age Frat a fone none araizsre doctor Su Pio ohe sel ea. rts pera! egrale stnino meta na igre smite spestosfepato a aprocso Qu “ign dl spi cibaiona, Lo tue esas gu ‘gr dl annals pu eseareeizztoinmmers compe: ‘ents parappotnaee eral segral a Turescncs “il otnat con matdo porta det sora esta & Inti prtco senmeesogerto par kt he Utzzaof ese pass ()una inate eg Dara eampon da amine marin ery care enous obgorlets complomera a rar | {lament do sequen wipe =i porcte pe- ‘pee dtemsate muazorsSNP no. Oui aa ‘his pode egrlepo abrcaion de ein Inoroseie a miaronsehPs « rai da gadogre ‘Seal pera conerzasone datacorvhPe no 2) ‘ra scored fave, da eopute slope qu camoion pst aaa’ d vations dala poral sgnaa, ‘ear un aay con gone Ste po ole feta as ato sro basen passa pest va int) fn ‘émat fal nogane pont dovt ametepiccauso cre ‘ignano aa cemplsco precoseo brane fomaco od prodot dl unghera eres curate fprocosse Femmentazere 391 carol fetmono carpine [adacor: eanblaront 3 sequaea ho Taerscon © ‘vane iavon a eaa sat vane do st ‘lois, prsaneaeoquone pti plein) ‘so appro segmunionore nde (2) cen Clruseta scons por aurora Speci © Sensis da ust ets che scan neo Iezarone dle condzani tiger temic ech (2) necssaaeampin cameos a fang (6) Searaestit deg ary. Teepologlemleroaray basa su tz ot ‘Abia vito sino ad ora metas ela i: a dllgorcleta ences sll specs acunped ST'DNA mara Ne suczasta paropa vero nace ano WA pase vaztasinvere eb ONA pale ‘at nl dengue Feseran a peta corglamertae regi ets OWA ONA ONAN Teenage misoaray cho utzzee la "pena eenson* par foirmas vorgote arial ven moa (reson ‘astersioneinsrgoonasacsdomedatedr peer tecnologia meray ‘a rasta ner lgarcia aap ‘oitezal (3) oar exonsne caorgorutetio ‘Sheps ra GNA tiaras es ‘oimers con annus bison (equa 9 Ezquenaloos apootia)Imetedipr cetera ‘Sruaarone SNPs tants eo ncn pesonaro ‘unrosvaron pet a ua basa uta iano. Ine gran a tet spestea dg enn ‘Ho sat. quest tol sora caratrazab Je Un BO pu steatzaia acca miseqiencra surangas pre opal & vetnno modems Vostromta 8, mode tle che fatmerzzazone, Uaonulete romps hello ‘tmuceoi adacn ala posse rtrd {0 PCR ron mares vare qund lato bie a ‘Sigenusnest La DNB pmo. pa lat 0 ‘ussca pe ovals dogredazone 35" digo ‘Side. ne ulzst prensa vn deoereseo {io (STP) maven aloe sto rss Nel feasonedpoimestzncaravenono acres? ‘guna eto dogg por eenrae 1 NTPs on ‘Seaport Laransone feata mogana meee Steoreeorza nquase odo oa GANT neo eran Raat rts Prt we, ‘itamert Suncare logon bs perio lponba “aranon a senna, conaae al miamo 838 sen ‘ots cn scraping, Sigal sour asec ‘ete ao sao trot prosaranno aun sgn Sondra Wont gt ao ak Con ave meta Trance cantare corte raat vor Pasi @ colar dl 197! dese 'o svupgo dea anes minaequenong ¥ pees Breage Ce LY Prod PCR | Ofeonctst Fedo ot nisonaranis proveoo We Saqun anal: (1) PER ‘rigor da NA genomic: (2 pepaavone cl prodat SPCR a sngie farmer: brcaone de pod oR aay) reason ot minsequensg su ray ‘redertoONA pleas risuasore dla uerence™ ‘Ziecauncsaercaron dr GaNTP ncaa che fe ‘Gros iovola! gnaipe. Cargaando mnsequenrg ‘inl nica taro passa emerge cei aps (88 dducrmnazone vaigenotpionongcs os etca9o0 ‘hamaroun on grande pa gene a mee ‘querang ache riniseuercng preset tavinae tin (1) copa tase dl deaturarone posto d PCR, IStensersa de thran DNA rain po’ ear lies aston sopgorscone&osegie una PCR B- rss supp com anna doar za amor non btn pele reazion mesa ‘At aut prepangor a ceadazonodt carers (st prosto POR medares apotone con ssrusoas., nen avo Ranero ¢ prt ae gup esteoat Ineo mediate pmo” uzza pra PCR (2) ‘toate che reconranonala mash 8 puro PC utc) a necsata a tmuver crwleument gt ‘lgoaeods es | aNTPs prime defesenon: (4) Bt ‘rer nat ala ONA pmo pve ats coo Sa (04-106) (yu tanoche ipod maaioneinuenca Ie pactotl da eazoe ron so as pte sh Sistema) (6) ls omacune str sorte 3a ince ch ieretacatat Sot sigonssenss rm asi (7) inpooobat euare a snes gh lonusectd prc qustaprocos da a8 enema Testamed 3 dalfaigpnoceioe tera po la suzzesva ‘Sioe. Keston clabraon nol 1903" hanna tuna wig Un tena cho porte Fivorsone oo ®t Reanpone elses iene dt mine ony .82 Sequansng, per quale matadca 8 ncsasars i 30 fetta arsed quatro die oiganaceai mst Iza pat quite, a roloe ate ce, geo is, he ancte un proto d esensore ps hnge ‘petal meiesqorang minemqanang Terenas oroigeracecioe sblespecice mostato conunque tra speatata smi, Teenage misoaray ce uz ‘peered aoe specemdta de DNA peneras sao slip un utorore mated lime oxen. sonal pion redata Gla NA plete” Guest 5 basa sla capacta, propa dia Tag pales. ‘Sermnare bra crron sora portanertscorglome" {ai arene pr stan una engaln bac in nn Zone etonoe denon pec raza ‘Staray 1d cngruooa50 ty earo ga leur ‘str asters, sao ears {Traetde compensa resale dao. IF , Ly Vex peaeali‘0 (Fa) carvione inst raprsenae da ONA ‘ing flares qustutina pus ess propa © Imadarte PCR asinmetcao moar apes cones" "las on laments dt pret PCR mor abo amen proto dala deacon dS gure fs teat (P10) od atectomié 8 deogorureatio Utzzio ela PCR La eane &eslonsene aware h [pecan dn 4NTP ono de qué melo (O}SEUTP), ‘touno tampa a ONA sang tamont con soqnes emplamenta iolgraeotse ize. Lae Slonsconend15cc drassonedenakrasorelegae (tron ostencona) © viene esapula eu veto Inedarto PCA at Nellvro ds roan e clatter 2001) @iponato un esonpio ot eriearone 48 SNPs mocon Dreson n'a sense tbe unos o> fama morons. € popra quedo varie cla Der eters aie speaticn media oa ONA palo eTipossa arate SNPremtadon nsequnas {Fn lngresza vara comprosa a 25 6 44M, ‘Queto conor att aeparmare # tang vo ‘Sptoaastmentn gabe PCA cho mm ota ape toto metcaray ulzzata neta Korean & mut ‘ontapaimertam: Arce uote toncapresena ci ‘socatmentadvus al rosotea al pot co ‘eantano nae rare! & estonsora dl per anche ‘Eero gue venir espa amen ao ‘ivdimaracazone ster 9 mewrosalsre deg olgens- ot ier gonson ese Wa esta dala Me Tecnologie miscanay “Single Bas0 Estnsin- TAGs (SETAC) ‘Un ers crap micoaay e etesona smuason epatortom erappesenaladaunmsedosho imbing Spock dle fas needa con Ter ‘sont Guta terol pu easte dlrs como ‘cake etrioe un singe aon o mise ‘quencing madata da DNA potmera La eazone ula Sloonasnos cet bara qui cet “ra coqionen on spec pe aga la sequoia ‘implomerarin grads svpporarea easier ‘Sone od ura soqunzn TAG (0 bacase op 8 sons egt suo) pr fr seve ores are ete ‘itary (ha), so mang velar arson pe Seam od onsen im ree dt Saray, sa ‘Sire d reason meisouuaneng fate aren cre ‘Somneamont, Le eoqueze TAG mipsorao a fossa {ester aray cen dcp un pasa nas ‘Seingioe rosso numero restr gent {azine sutaoo ch prevecoe Tuo 8 era cae ‘une dl mineoquencing. Tecnologie merwaray ch utzane razon medae da Onaga ‘Quest tecnicos bane su na reason medias a oA iga! cho even a ONA 6 i olor ‘mobizaa, In Fgwn 10.8 roan 9 sokena a ‘enon OLA La enon shan ura sop igen ‘Seo alte speotes por og munsanaSN? seae iesoun lgorceotdopetaind trletincaatestco ‘died var sloth AI DNA sso anera8 lope wo oe cu lgenot ate spon! og leo Inrlotdeigndo spe mein sacar a mse ‘sonora a sta detrsee Se @potatacomo ‘mental taghcigon secs ea 0NA BONA Noa p> ‘Spotwela son ised aera un sana Here 3 nso ran o peat complements asl ot [peoee leigenuSntte endo pei mareat gaa ‘ar: no aver gur i sopra iva ‘Con ueata crag mecearay etna ie cient’ final og tanmaro & ONG da are Sre otense medaredigestoncon Oras. La esto ‘Sirmceapegwomurapipasora dexge ‘Gs sudare, Dopo afore oct ala ma. ‘Shura dl sequanes gra © zsaro oignaat- SF immotizassuaray costa crea 20 basa Sona costar equates ancnapp0) edn bs (0 Sjoompemensr ato equnea ames uo rear aa oY aw Feedeusne scans date wera monary 5: ‘REE SSecenge base mors SNPs meee paLa tecnologia meray ur ‘haa na aro, nd OM oa, ra veanpere @ _psgonmices oennicano ary Nse sce low some 3 fe posi sostuzet (Fg. 11). Un ‘lgoueote completa ala sequaca ancora ‘90 vino io m rede Ga generar un ido con a dn 8 on! oem'n onan ace cameo pt ‘gor tlgare det mlcol DNA atest Solo- "ate. Uando dt paretl dh elgnacieoba conte Dosh pete, quosto metodo et most velo pla ‘Solesrara mason an oe Thaueetlo asa, un veut accurate pc ee- sec en compsran fescnzs mest dua {etsy a pana de unk bdo con un ONA a "Tomer re soands eon ONA dl campene cx ‘alas (P92, eda compara lina gna ‘Sirlondont aot eve dase coc © re ‘Soon maple OA sigenasecie™ ‘uosa acca pesria sequent vartsgp: capac i dlcalazr ed entteae noe maz aps {is suave vats ONA fina‘ 2 Icon laa ‘scovratezra (cra -00%) ears coramporn econ root PCR mute ‘TECNOLOGIA MICROARRAY BASATA SULL'APPLICAZIONE DEL CAMPO ELETTRICO A fine superar ale lint emer net pp sion dal acne mcroaray pa dein & Ita pelimoram @ sata eentrerteavtppas na metosea microaray (aogen) ha ura caret ‘Set por espa apd eg acu dee nce olgene Gancne as arate rood © pd) de hp che per neemertar einen de eo [Bigazone'? Gua ates, ote alastngenca rica Ssimica, viz gts tronien = pron nna Tesazonestve"** 1 marscnp hanno dmotio ‘ina ome person conente 210040 i ot: ono prio store ary Sx, 1x10 920207 13). leo soolealzz nt oon cats daray ono hn anata Opn at ae Sree oatonce mavaian osc ane ‘Sip Male Boy Woratonytwasprssltno olden a proaat a PCM vera seams plano una corents posta almrsltod sleo- ‘aray éxito mato loch vrs morse ted sue sua supers possoro esate soketararie ‘area agabvamertopstvanet. O37 eames Set ‘hip gana indents dg at “Gabe sqm cratreo, a eenlaga Nanopen tenors arg (1) Fz dla sngoea det tonen parma aggurgerele once basaone ‘emai por SNPainfazon vor 2) campo eco Scorn modo spies pane mabe an tt a vee dl process: Msn sane “ar anechein ae (9) eaomarete ects gates taspano adele det garcia de podoPCR ‘pesto specie dara una tecnope ver ‘itl quai op sto testa 8gonaete aap ak Siesortperato lo sto rarmone mlacars SNPs och sgt op atazarlPan rumor ar fo () pub ese ulate et analazre gi ‘rowed biege datale cares sete mole ite appleab tere banadex, dopostca, ganar (a amocogencca.La tecnologia mew "eanpo site, a campestone dle sao Inidatne oa pocrza dott pes utars ‘Sendai mporancaprieepeezora dross ‘Stsarione atv eanpo eee era rls Sains rmansur ‘daca apenas permet ‘opal trgeran medal vtaronedeacuatoren (Sengorza strona). La oz de! cargo ait 8 on Sr rears pe ogra a endo ‘dono 8 rappresntta destin 5 min 0 ra ‘tampons amet pr ereason dl adora naa Impodece bus sabia pt ucsea app one del cargo sets pando moe ease '# raasone) ad ha ina corte moto basen {1005 tvs para I owen la ele foie acolrando tproosse t eaora™ Uo sto emeable (1-10 en), oxi da apecsa © peter ‘Shloi, semata geet (F 4) eda ance Demene 1 easpro oleic (b100 nA 0 V2 Deeg reac a ize gt lt elton “mos! ahaa siperioe place dt mercatiogo (Grotusone ion HO. lt OM 6 adeat te) © Ania da matics pr fate des post PCR De lan quae corsena aide © sepisnm. Oued {Fase areraciora speci va bot © rota, tha ota che prods PCR sora at asp au Uno Specto stot, mangora sana ge, "Nutro arcana aNaneip woe Bi ‘egy Weritaton en hip (Naneip Crag) con "00 2 tn, I nanbo gun he soupy corals © ‘hmzzio una melaiouge pero stato sage sel rumereseSNPutrascor rasa cts gens Ua “alone ata meteden 6 et sop 50 sggst (Geqsenionano in storste, anal dl estitanee Scrminaiene atc) megane lo tudo 18 var ‘ora, peril 2250 carters Inbrove inal ia mi ro dP cor ‘Suse rebotzaa,donausal © Yasar ltrnen ‘ete apace soos. nse iene dl gore = (asa eurtzzo do sonde (012 ban) spear por (mutazonatSNP che deere side ne on iron open aca en ‘mateata con Oy Oop # asp stro do prodot PCR ena eeapula ics sone cores [atnzzare (250 bas atu, Sogn na. iro afesomta 3 doa sora, ba 3 pode me cosas ura sano “basen 5) ‘clusvarerte con a son pettamere conpaent ‘@alprooto PCR, ratzsndone toga Seando ‘nln tore Wao cant pe a erin a sonco poratarans congeners oon La dest ‘oowe du sande, eeguta medrese a ase sua vor Rabin ge cones a PPodoto dl PCR spe ala onda non peetarate Femplomertse (Fp. 1, amelie, eos me ‘rola pamete detamnare ea cspone cee ‘9 Sprin ewe ‘etary oo ace care at pra a pss PCE Saaremaa Eneyia Base SACkiNe Gustto aN -_ Reporter » cenmennf sent) » > GSREMERRETEDME) 5 Foun 6 EES nase acl ates eactctar a ae ‘ann arn ea cunt rreaanscorpanata oun rk dy Serene rn ‘tr te emt eeLe tceooga mroaray Seavey ceca eo st, eagaty slo nso ra nse Ba fake canon apis coo “alle= ‘2 0 a expats pe ree pert: nora ‘Sirota nga svor c at tuoi 6), oS ettan (Fe, 17.peganina Por Tana aa sega etrada i genipo & Shecucmge F918 Cano oe a zone dol soins, La strazone de rumor @ endo ‘naps sin ao eg crete reds PCR. Dae ana! al pio SNPIntazion, onde sno alrtanate ppg) das to monte incenenoatimprara oscuzere acalna ed orpote leap Vene poaro sagt endaiona con sone lo saezsore spc po sac usonalsAP SSiverete.Ipeecote desta & spa poo sso te rata hPa ies. roots stnare he ronan isuseg naps process bedazoraeppng non sb. 1 svt un rent ere de anh ste cago, pared oe pea PCA ra caso 4) anal 5 vara gene pr icon eat 70:02 tote de op, Pont, nent gutta enon {08 govern pe ry), Timezone dpe ‘alo spemertl ao eet seca an ory "saa rat prepa erry Ae (Peder qv metic oe ave fequensamet ‘Momates ena eso euserminate ales) {poem corcordnca ose coeria pentane isi scarp ena. gt rach ora nea aprons trndo reset he (i) dsogn dete onde, a sun vl eporeale daa Scuatrza dla endoa, © spose (2) po” Pte Su ataso ao ea: quo acsbanenone compra ta pl cata vlaason tale dl preoclo spe intl atbane fa ala stgenee stones ‘temo ara maggie accurate rl caatazanon Sete, rae nasa roe movers Sn Le dvewetcnonge macory tora eso ‘ove, sore aban og ose appreseriaba te 1 possi mtosche meoaray 2 dponbl pt lo Sidra, an art ate scote dale cng pu Koa a soto 1 Popo eager sano ose reached agnosia. ‘ogg tamte ene soso tute questa tase 6 ‘eopecti dala impossa ezato I DNA geno Io detaont su chp Non pra ancora posse ‘artes anasto qual PCRefuticazon comp ‘Nalmomaea eso cl favaenmen ter. 3) Censored tlzare 1 DNA genio eretarents au tstenamgaa se te tcaogereamer BIBLIOGRAFIA 1. Nason IK, Fore 0, Asie Ue a Sine te era ig of DNA 2 Maa JG. Resetecing and minal ays wing 3. Maa 16, Bray LC, Chee MS, ea: Dost of 1 Kelathowskd M, Preson §,takson Ayspect, hightroweiut genome by alle spec 7. Redon F, Khmer R Mann W, a Det of 8 Fam 5, Chen X, Hasta MK, Berne A et ab Nbdenyolesolode tg saps Cerone es 10, Gunderson Li, tng XC, Mori MS, tal Par cosponsors Sabina Frscons ‘nt Operatin Goeton«Caegenton ‘AzendaOspaer Caren Vale Worpage 05 te Penge Ta: 055470989 Fx 0554270688 stecnologiamiroaray ee BB, Lin SW, Vide J a Beco aman CF, Renn DE, Wa Dt a Hee eld Gee C, Ta Jama, ab. Micke allo, Bec 00225 1563.50