CH PHM THUC TR SU SINH

HC T R V V CY CHM RUT
(PHYLLANTHUS ACIDUS)
Nguyn Th Chin1, Nguyn Vn c2,
Nguyn Hi ng2, Thi Tho2
TM TT

S dng thuc tr su ha hc nh hng ti sc khe ngi dng

tc ng xu i vi mi trng. Mc tiu ca ti l iu ch ra dung dch c
kh nng tiu dit cc loi su hi cy trng t v v r cy chm rut. ti tin
hnh thc nghim quy trnh ly trch c t trong r v v cy, tin hnh xc nh
LD50, LD100 ca dch chit trn cc mu su hi, ang tin hnh th nghim trn mu
i chng cy trng.
T kha: Chm rut, r , v cy, thuc tr su sinh hc

I.

TNG QUAN V CY CHM RUT

-Tn gi khc: Cy Tm rut, Cy Tm

rut.
-Tn
ting
Anh: Otaheite
gooseberry, Malay gooseberry, Tahitian
gooseberry, country
gooseberry, star
gooseberry, West
India gooseberry,
simply gooseberry tree.
-Tn khoa hc: Phyllanthus
acidus (L.) SKEELS.
-Tn ng ngha: Ph.
distichus; Cicca acida ; C. disticha; Averrhoa acida.
Thn: Chm rut l loi cy thn g ln, t chiu cao trung bnh 4-6
mt, cy cao nht c th t n 10 m. C tn rng v hoa mu hng rt p,
thng c trng nh mt loi cy cnh sn nh hay trong vn.
Thn cy c g b, nhiu cnh mc t thn chnh, cnh gin d gy.
Nhnh cy sn si v vt so ca nhng cung l c. cui mi cnh
chnh c nhiu cnh nh mu xanh, di t 15 - 30cm, mc thnh chm dy c.
-L: L kp mc so le, l cht hnh trng, di 4 - 5cm, rng 1,5 - 2cm
1
2

B mn Ha-Trng THPT Chuyn L T Trng-TP Cn Th

Lp 11A4- Trng THPT Chuyn L T Trng-TP Cn Th

II.
III.

NGUYN LIU V PHNG PHP

Nguyn liu
R v v cy (3-5 nm tui) c thu ti khu vc Bnh Thy- Thnh ph Cn
Th vo thng 9/2013.
Chit xut v c lp
Giai on 1: Chun b nguyn liu
-

Chm rut ch ly phn v v r cy.

Phi kh

Hnh 3 - V chm rut

Hnh 4 - R chm rut

III. S TCH CHIT

S tch chit

V chm rut

R chm rut
2

Cho nguyn liu phi kh vo cc bnh nha, ghi tn.

Dung mi : cn cng nghip (ethanol 960 )

Ngm v v r chm rut vo cn trong 1 thi gian (15 ngy).

Lc ly phn dung dch.

C cn bt cn thu c dung dch bo ha.

Hnh 5 Ngm v, r chm rut

IV.

Hnh 6 - Dung dch ban u

Hnh 7 - Dung dch bo ha

THC NGHIM

Chun b cc mu th (su go, 50 con/ mu)

Pha long dung dch bo ha vi nc theo tng t l th tch khc nhau, sau
phun ln cc mu th, ghi nhn thi gian khi phn na s su (LD 50) v ton b
su (LD100) trong mu th cht ht.

Tip tc th thuc trn su hi rau ci (phun ln rau, th thuc trc tip trn
su)

V.

KT QU THU C

Kt qu th nghim vi su go

Hnh 8 - Cc mu su go phun thuc trc tip

Kt qu th nghim vi dung dch t v chm rut (Mo)

Bng 1: thi gian 50% s su trong
mu cht
T l th tch
Thi gian 50%
(M0/H2O)
su cht
1:10
1:20
1:30
1:40
1:50

1 pht
1 pht 15 giy
1 pht 35 giy
1 pht 50 giy
2 pht 30 giy

Bng 2: Thi gian ton b s su trong mu cht

T l th
tch
(M0/H2O)

Biu 1 - Mi quan h gia th tch v

thi gian 50% s su cht (bng 1)

Biu 2 - Mi quan h gia th tch v thi

gian ton b s su cht (bng 2)

Thi gian ton b

su cht
(LD100)

1:10

3 pht

1:20

8 pht

1:30

15 pht

1:40

20 pht

1:50

29 pht

 Kt qu th nghim vi dung dch t r chm rut (R0)

Bng 3: thi gian 50% s su cht

T l th tch
(R0/H2O)
1:10
1:20
1:30
1:40

Thi gian 50%

su cht
LD50
2 pht
3 pht 30 giy
4 pht
6 pht

1:50

10 pht

Biu 3 - Mi quan h gia th tch v thi

gian 50% s su cht (bng 3)

T l th tch
(R0/H2O)
1:10
1:20
1:30
1:40
1:50

Thi gian ton

b su cht
(LD100)
30 pht
32 pht
40 pht
47 pht
50 pht

Bng 4: thi gian ton b s su trong mu

cht

Biu 4 - Mi quan h gia th tch v thi

gian ton b s su cht (bng 4)

Bn lun: Dch chit t r v v cy chm rut c tc dng mnh i vi su

hi cy trng, tuy nhin nhn thy qua biu thng k LD 50 v LD100 thi tc
dng ca dung dch chit trong v cy c tc dng tt hn so vi dch chit t
r.

VI.

KT LUN
S dng thuc tr su ha hc nh hng ti sc khe ngi dng tc
ng xu i vi mi trng. Theo cc ti liu y hc, trong v v r cy chm rut
c cha cht c, cc cht c chit xut t v, r cy chm rut cng c kh nng
tiu dit su hi trn hoa mu. Trn th gii v trong nc vn cha c nghin cu c
th no v vn ny. Mc tiu ca ti l iu ch ra dung dch c kh nng tiu
dit cc loi su hi cy trng t v v r cy chm rut. ti tin hnh thc
nghim quy trnh ly trch c t trong r v v cy, tin hnh xc nh LD 50, LD100 ca
dch chit trn cc mu su hi, ang tin hnh th nghim trn mu i chng cy
trng. ti thnh cng gip cho ngi dn c thm mt la chn bo v ma
mng, mi trng, ngun nc an ton cho con ngi trnh c s nhim ca
thuc ha hc. Hng pht trin ca ti l tin hnh phn lp cc hp cht c trong
r v v cy gp phn vo vic bo tn v pht trin mnh loi cy ny.
TI LIU THAM KHO
Huy Bch (2004), Cy thuc v ng vt lm thuc Vit Nam, NXB Khoa hc v K
thut, tr. 842-843.
Mnh ng, KC.04.17: Cng ngh mi lm "sng dy" nhng bi thuc qu c truyn, Bo
Khoa hc v Pht trin, ngy 20/12/2006.
Nguyn Ngc Hnh (2001), Tch chit v c lp cc hp cht thin nhin, Gio trnh cao
hc.
Tt Li, 1995. Nhng cy thuc v v thuc Vit Nam, NXB Khoa hc v K thut, Vin
dc liu, trang 720-722.
Nguyn Khc Qunh C (1999), Bi ging chit xut dc liu.
Nguyn Kim Phi Phng (2005), C lp chit xut hp cht thin nhin, Nh xut bn i
hc Quc gia Thnh ph H Ch Minh.
Chung V cng cng s, Nhng cy tinh du Vit Nam, khai thc, ch bin, ng dng,
NXB KHKT HN, 1996.