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2 drops
NOTE: When used as a counterstain, this stain should be diluted 5-10-fold in acid alum (the
base without the dye)
Stock Congo Red Solution (0.25% Congo Red in 80% EtOH)
Solution may require several days of stirring to dissolve (one day minimum); even with
protracted stirring undissolved particulates will remain. This solution is stable for six months.
100 ml
400 ml
15 g
1.25 g
H2O
Ethanol, 200 proof
NaCl
Congo Red dye (Sigma #C6277, histology grade)
Procedure
1. Filter hematoxylin solution using fluted Whatman paper.
2. Rehydrate sections in running lukewarm tap water for 20-30 minutes.
3. Stain in 10-fold diluted hemotoxylin for 15-20 minutes.
4. Rinse in running lukewarm tap water for 5-15 minutes.
5. Stain in congo red solution for 50-60 minutes.
6. Rinse in running lukewarm tap water for 15-20 minutes.
7. Dehydrate through an ascending alcohol series (70%, 95%, 100%) into xylene.
8. Coverslip with Permount.