MANUAL BioCommand Lite BioProcessing Software PDF

Guide to Operations BioCommand Lite BioProcessing Software BEFORE INSTALLATION See Chapter 1 ___ al MANUAL NO: M1286-0050 Revision A January 4, 2002 NEW BRUNSWICK SCIENTIFIC CO., INC. BOX 4005 ¢ 44 TALMADGE ROAD ¢ EDISON, NJ 08818-4005 ‘Telephone: 1-732-287-1200 ¢ 1-800-631-5417 Fax: 732-287-4222 ¢ Telex: 4753012 NBSCO Internet: http://www.nbsc.com ¢ E-mail: bioinfo@nbsc.comBioCommand Lite M1286-0050 Users Guide(TERNATIONAL OFFICES: ‘THE NETHERLANDS ‘New Brunswick Scientific BV Kerkenbos 1101, 6546 BC Nijmegen P.O Box 6826, 6503. GH Nijmegen Holland Tel: (0)24 3717 600 Fax: (0)24 3717 640 E-mail: sales@nbsby.nl FRANCE, New Brunswick Scientific SARL. 3, me des Deux-Boules 75001 Paris France Tel: (0)1 4026.47 06 Fax: (0)1 40 26 $4.23 E-mail: sales@nbssarl.fr UNITED KINGDOM New Brunswick Scientific (UK) Ltd. Edison House, 163 Dixons Hill Road North Mymms, Hatfield, Herts. AL9 7JE United Kingdom Tel: (0)1707 275733, Fax: (0)1707 267859 E-mail: bioinfo@nbsuk.co.uk CHINA (Beijing) Room $-3-B. No. I Heng Fu Zhong Street Feng Tai District Beijing 100070 P.R. China Tel: 106371 0167, Fax: 10 6374 1107 BELGIUM ‘New Brunswick Scientific NV/SA “tVeldeke, 1 B-1970 Wezembeek-Oppem Belgit/Belgique Tel: (0)2 731 6787 Fax: (0)2 731 8130 E-mail: sales@nbsnv-sa.be GERMANY New Brunswick Scientific GmbH In Der Au 14 1D-72622 Nortingen Deutschland Tel: (0)7022 932490 Fax: (0)7022 32486 E-mail: sales@nbsgmbh.de CHINA (Shanghai) Room L406, Aijian No. 1 Bldg. Lane 590, WanPing (S) Road Shanghai 200030 P.R. China Tel: 21 6481 2658 Fax: 21 6481 2665 E-mail: nbsche@online.sh.cn New Brunswick Scientific Users GuideTHIS PAGE INTENTIONALLY BLANK BioCommand Lite © M1286-0050 User's GuideWARNING! This product must be operated only as described in this manual. If operational guidelines are not followed, equipment damage can occur. Please read and understand this User’s Guide before attempting to use BioCommand Lite. New Brunswick Scientific User's Guidevi THIS PAGE INTENTIONALLY BLANK BioGommand Lite @ 1286-0050 Users Guidevii Copyright Notice New Brunswick Scientific Company, Inc. Box 4005 44 Talmadge Road Edison, New Jersey 08818-4005 © Copyright 2002 New Brunswick Scientific Co.., inc. Ail Rights Reserved. . Reproduction, adaptation, or translation without prior written permission from New Brunswick Scientific is prohibited. Disclaimer Notice New Brunswick Scientific Co., Inc. reserves the right to change information in this document without notice. Updates to information in this document reflect our commitment to continuing product development and improvement. Manual Conventions ‘Baier Notes contain essential information that deserves special attention. Caution messages appear before procedures which, if caution is not observed, could result in damage to the equipment. CAUTION ! Warning messages alert you to specific procedures or practices which, if not followed correctly, could result in serious personal injury. WARNING ! Bold Text in bold face type emphasizes key words or phrases. New Brunswick Scientific Users Guidevill THIS PAGE INTENTIONALLY BLANK BioGommand Lite M1286-0050 User's GuideWARRANTY PS Every Instrument manufactured by the New Brunswick Scientific Co,, Inc. is warranted to be free from defects in material and workmanship. This apparatus, with the exception of glassware, lamps and electrodes (where supplied), is warranted for | year against faulty components and assembly and our obligation under this warranty is limited to repairing or replacing the instrument or part thereof, which shall, within 1 year after date of shipment, prove to be defective after our examination, This warranty does not extend to any NBS products which have been subjected to misuse, neglect, accident or improper installation or application; nor shall it extend to products which have been repaired or altered outside the NBS factory ‘without prior authorization from ‘New Brunswick Sciemtfic Co., Inc. ‘New Brunswick Scientific Users GuideTHIS PAGE INTENTIONALLY BLANK BioCommand Lite © M1286-0050, Users GuideTABLE OF CONTENTS 1 READ THIS FIRST. 1.1 BEFORE INSTALLING BIOCOMMAND LITE.. 1.2 OBTAINING-A PRODUCT REGISTRATION NUMBER, 1.2.7 Online Registration... 1.22 — Registration at Local NBS Sales Office 123 Registration at NBS US. Office 13. LICENSE AGREEMENT ACCEPTANCE svn 1.3] New Brunswick Scientific Software License . 13.2 US. Government Restricted Rights 2 INTRODUCTION. 2.1 MAJOR FEATURES 2.2. PRODUCT BENEFITS 221 General 2 Displays 223 Application. 2.3. How 70 Use Tuts MANUAL 24 ORGANIZATION OF THIS MANUAL 2.5. NOTATION CONVENTIONS USED IN THIS MANUAL 25.1 Mouse Notation. 25.2 Keyboard Notation 25.3 Menu Notation 254 Window Element Notation 2.6 NAVIGATING THROUGH WINDOWS®. 2.7._ REFERENCE FOR ITEM NAMING CONVENTIONS. 7.1 File Naming Conventions 3 BIOCOMMAND LITE INSTALLATION & SET-UP. 3.1 Compurer Setup 311 Windows? 98. . u 31.2 Windows NT R 31.3 Windows 2000°. 2 314 Windows ME* B 3.2. STARTING BIOCOMMAND LITE aimee 3 3.3 RECIPES AND BATCHES. 4 5.8 “STARTING BIOCOMMAND” SCREEN : ‘ 4 4 Tiring te String Riad Screen Off & On oma ‘ 15 3.5. MAINMENU. emit 3 3.6 MaIN TooLBaR zinc i 16 4 NBS HARDWARE CONNECTION SETUP. 4.1 CABLE CONNECTIONS. 411 Required Hardware 412 Making Connections 4.2 SETTING UP CoMPUTER/CONTROLLER(S) COMMUNICATION... ‘4.2.1 NBS Hardware Connection Setup. 42.2 Select Comm Port 423° Controller 1D List 424 Controller Staus. New Brunswick Scientific Users Guide5 CREATING A RECIPE & STARTING A BATCH 5.1 CREATING ANEW RECIPE USING BATCH WIZARD 522. EXPANDING A BASIC RECIPE. 33. ALTERNATIVE METHODS TO CREATE A NEW RECIPE 5.31 Howto Access Batch Wizard. 35.2 How to Access the New Recipe Startup Screen . 32 5.4 STARTING A BATCH. : - Be Ras, 838 SA.) Selecting a Recipe. 7 38 542 Detar a Recipe or Batch : eee eae: a $43 Starting the Batch from a New Recipe : al 5.44 Starting the Batch from an Existing Recipe : : 2 545 Starting the Batch from an Existing Recipe with Changes B 6 BATCH VIEWS.. = 4s 6.1 BATCH SUMMARY VIEW, 62 TREND VIEWS 21 Creating a Trend View. 622 Using the Trend Graph. 623 MAIN Control Panel Tab. 62.4 LOOPS Control Panel Tab. 6.2.5 GRAPHS Control Panel Tab 626 STYLES Control Pane! Tab 62.7 Modifving a Trend View 7 ALARMS.. 7.1 SETTING ALARMS 8 ENDING A BATCH. 8.1 ENDABATCH. 82 EXITING BIOCOMMAND LITE. 83. RESTARTING WITH ACTIVE BATCHES... 84 SHUTTING DOWN BlOCoMMAND LITE, 9 MAIN SCREEN FUNCTIONS, 91 FILE. 92 EDIT 93° View 94 Tools. 9.4.1 Offline Data. 9.5 Winnow 96 HELP 9.7 TOOLBAR DESCRIPTION. 10 RECIPE/BATCH FUNCTIONS. 10.1 CREATING OR EDITING RECIPES. 10.1.1 New Recipe Setup Window. 10.1.2 View Recipe Setup Window. 10.13 Using the Setup Window. 10.14 Recipe Tab 10.1.5 Loops Tab 10.1.6 Duplicating a Recipe. 10.1.7 Views Tab. 10.1.8 Alarms Tab. 10.2. STARTING A BATCH. 10.2.1 Batch Wicard. Biocommand Lite M1286-0050 Users Guideail 10.2.2 New Recipe or View Recipe es ‘ os 96 10,23 New Batch. 96 10.3. ALTERING A BATCH IN PROGRESS 97 10.3.1 Changing Setpoint. 7 103.2 Editing Trend Views. 98 103.3 Adding Views. nn ae -_ 99 1034 Deleting Views 99 10.3.5 Ending a Batch . sone 100 103.6 Resuming a Batch fn i 101 103.7 Changing Data Logging Criteria 7 2 es msstcssiin 10, 11 TROUBLESHOOTING . 111 TROUBLESHOOTING GUIDE 12. SPECIFICATIONS & SYSTEM REQUIREMENT! 12.1 BIOCOMMAND LITE SPECIFICATIONS. 122. SYSTEM REQUIREMENTS: 2.) Minimum Computer Requirements 122.2 Recommended Computer... 12.2.3 Computer Operating Systems 13. DATA LOG FORMAT. 13.1 SETTINGS, 13.1.1 AlarmSertings. 13.1.2 CascadeSettings. 13.1.3 StorilizeSettings. 15.2. CHANGES 13.2.1 ChangeRecord. 13.22 MgrChanges 13.2.3 OperChanges 13.24 SupeChanges. 133. BATCH EVENTS... 13.3.1 BatchEvents 134 REPORTS 13.4.1 ReportGraphs 13.4.2 ReportSettings. 13.5. Dara Loss... 135.1 Loop Data. 135.2 LogTimes 135.3 OflineData 13.6 BATCH IDENTIFICATIONS. 13.6.1 BatehiD. 13.6.2 Loops 13.6.3 CurreniStatus 15.7 Errors. 13.8 VIEWS DATA... 14 VIEWING BATCH DATA. 14.1 STARTING DBVIEWER32. 142 ExisTiNG BaTcHEs. 143 USING THE MAIN Data WINDOW, : . 116 14.3.1 Browse Button... ae 116 14.3.2 Batch Selection Box ei ui? 143.3 Settings Buton. WT 143.4 Export to Excel Bution. se 19 143.5 Central Data Pane. se ? 120 New Brunswick Scientific Users Guide20 xiv 125 143.6 DBViewer Main Memu 144 MORE BATCHES 1S INDEX. Users Guide Biocommand Lite M1286-005011 1 READ THIS FIRST Before installing BioCommand Lite BioCommand Lite is copyrighted software and may not be used without a license Before you do anything else, read this entire chapter. The license supplied with your purchase is good for a networked installation at one single site. Before BioCommand Lite can be used, you will need to obtain a Product Registration Number from New Brunswick Scientific 2S. NOTE: 1.2 From the time you receive your Product Registration Number, you may use it to activate BioCommand Lite during a period of two weeks. If you are not ready to install and activate the software, do not request the product Registration Number at this time. “The number of Product Registration Numbers that New Brunswick Scientific will issue against one BioCommand Lite serial number is limited. Be sure you aze ready to proceed with installation before you request a Product Registration Number. Also, be Sure to save the BioCommand Lite serial number in case reinstallation becomes hecessary, because a Product Registration Number cannot be issued without the serial number (which is found on the back of the CD case). Obtaining a Product Registration Number ‘The Product Registration Number can be obtained in several ways: «Online through the worldwide web (the fastest and easiest method to use in most cases) © Through your local NBS sales office, by e-mail, phone or fax Through our U.S. headquarters, by e-mail, phone or fax Contact this website: http://www.nbsc.com Fill out the online Registration Form. You will be asked for: New Brunswick Scientific User's Guide1.2.2 41.2.3 1.3 your name and title your “Institution” (the company, institution or organization where you work) your institution’s street address, city, state or province, and country your work phone number and e-mail address the serial number of your BioCommand Lite software, which is found on the back of the CD case. Registration at Local NBS Sales Office Contact your local NBS sales office by telephone, fax or e-mail, and provide the information listed in Section 1.2.1. Registration at NBS U.S. Office Contact NBS headquarters: ‘* by telephone: 1-732-287-1200 © by fax: 1-732-4222 * by e-mail: bioinfo@nbse.com to provide the information listed in Section 1.2.1. License Agreement Acceptance ‘You must also accept the License Agreement below before you can use this NEW BRUNSWICK SCIENTIFIC CO., INC. LICENSE AGREEMENT FOR BIOCOMMAND LITE This is a legal agreement between you, the end user, and New Brunswick Scientific By obtaining an installation password and installing this software, you are agreeing to be bound by the terms of this agreement. If you do not agree to the terms of this agreement, promptly retum the package and the accompanying items to New Brunswick Scientific BioCommand Lite M1Z86-0060, Users Guide4134 1.3.2 New Brunswick Scientific Software License 4. Grant Of License New Brunswick Scientific grants you the right to use the BioCommand Lite software program (BioCommand Lite) in a networked environment ata single site. You may Tastall BioCommand Lite on multiple computers within a single network domain, ‘You may not install this copy of BioCommand Lite in more thart one network domain at the same time. 2. Copyright The BioCommand Lite program is owned by New Brunswick Scientific or its suppliers and is protected by United States copyright laws and international treaty provisions. Therefore, you must treat BioCommand Lite like any other copyrighted material (e.2., a book or musical recording) except that you may make one copy of BioCommand Lite solely for backup or archival purposes. 3. Other Restrictions You may not rent, lease or transfer BioCommand Lite. You may not reverse engineer, decompile, or disassemble BioCommand Lite U.S. Government Restricted Rights The BioCommand Lite software program and documentation are provided with RESTRICTED RIGHTS. Use, duplication, or disclosure by the Government is subject to restrictions as set forth in subdivision (b)(3)(ii) of The Rights in Technical Data and Computer Software clause at 252.227-7013. Contractor/manufacturer is New Brunswick Scientific Co.. Inc./44 Talmadge Road/P. O. Box 4005/Edison, New Jersey 08818-4005, USA. “This Agreement is governed by the laws of the State of New Jersey. Should you have any questions concerning this Agreement. or if you desire to contact New Brunswick Scientific for any reason, please write: New Brunswick Scientific Customer Sales ‘and Service, 44 Talmadge Road, P.O. Box 4005, Edison, New Jersey 08818-4005, USA. New Brunswick Scientific Users GuideTHIS PAGE INTENTIONALLY BLANK BioCommand Lite @ M1285-0050, Users Guide2 INTRODUCTION BioCommand Lite software enhances the capabilities of NBS Bioprocessing equipment. It oversees the independent operation-of one or many NBS process controllers. BioCommand Lite eriables centralized control, monitoring and data acquisition in applications ranging from a single benchtop laboratory fermentor through arrays of large-scale production vessels. This software uses industry-standard conventions, including the familiar terms of Recipe (all of the variables associated with a fermentation run) and Batch (an active application of a recipe) Major Features Centralized monitoring and control of fermentors/bioreactors (from one to eight units) Automatic process data recording Clear graphical numerical displays Product Benefits General BioCommand Lite is a Windows®-based, multi-bioprocess supervision program. It is designed primarily for use with New Brunswick Scientific’s fermentation and cell culture equipment, but can be extended to the control and supervision of other equipment as well. This software uniquely retains the easy- to-use character of its precursor, AFS BioCommand, while adding 32-bit architecture; expanded graphing capabilities; and recipe-based process control. ‘We are confident that the power, flexibility and convenience of this software will enhance creativity and productivity in laboratory, pilot, and manufacturing applications. Because BioCommand Lite is software that can be validated, itis suitable for use in FDA-validated processes. BioCommand Lite runs under the popular and familiar Windows XP°, Windows XP Pro®, Windows ME®, Windows® 2000, Windows NT® and Windows” 98 operating systems. New Brunswick Scientific Users Guide2.2.2 Displays BioCommand Lite provides easy to use tabular and graphical displays of process data. 2.23 Application BioCommand Lite is compatible with all New Brunswick Scientific computer- capable products. The number of units supported is limited to eight. 23 How to Use This Manual We recommend that operators first acquire skill with the controllers, then the software. ‘The overlap between some BioCommand Lite functions and some controller functions can steepen the learning curve for those trying to learn both simultaneou: Follow the first three chapters carefully to ensure that the software is installed correctly, and the appropriate interface connects the controlled instrument(s) to the host computer(s). These initial steps are best performed with the assistance of MIS personnel. Refer to Chapters 3-8 to set up and complete a first BioCommand Lite run. 24 Organization of This Manual Chapters 1-2 ‘Chapter 3-8 Chapters 9-10 Chapter 11 Chapters 12-14 Introductory material. These chapters take the new user from system set-up and program start-up through fermentor shutdown in step-by-step tutorial style. They include sufficient detail to enable further independent exploration. Reference chapters containing in-depth explanations of features. A troubleshooting guide to help you diagnose and resolve some potential difficulties. Detailed information that is usually not necessary for routine program operation (product specifications, system requirements, data log formats and instructions for using the DB Viewer utility), but which may be helpful to advanced users. BioCommand Lite © M1286-0050, Users Guide25 2.5.1 26.2 2.5.3 2.5.4 Chapter 15 ‘An index to key terms used throughout the manual. Notation Conventions Used In This Manual Mouse Notation Notation Application Glick Press the left mouse button once. Double Click Press the left mouse button twice, in quick | succession _| [Right Click Press the right mouse button once. Double Right Press the right mouse button twice, in quick ick succession. : Keyboard Notation Keys are displayed with their keyboard label between the “<” and “>” symbols, such as (Escape) or . Modifier keys (, , ) which are pressed along with another key are also shown between the two symbols . The modifier is separated from the other key by a dash, For example, means that both (the Control key, which is labeled Ctrl) and (the key labeled D) must be pressed simultaneously. If modifier key(s) are required, press and hold down the modifier(s) while pressing and releasing the other key. ‘Then release the modifier key(s). If two modifier keys are required both are shown within the symbols < >, and are separated by dashes, as . Function keys are displayed with an “F” preceding a number: . Menu Notation Menu items are described by the selection sequence that accesses the item. A dash separates items in the sequence. For example, the Save function under the File menu is written as File ~ Save Window Element Notation Window names (in the window title bar) are enclosed in double quotation marks, as “Loop Setup Options”. © Tab labels are also enclosed in double quotation marks: “Recipes” New Brunswick Scientific Users Guide26 © Data-eniry-field names use single quotation marks, as in ‘Loop’. © On-screen (virtual) Button captions are enclosed in curly brackets “{ }". Navigating Through Windows® Skip this section if you are very familiar with the Windows® Operating System. Those in need of a review should read on, For more information, consult Microsoft or third party manuals on the use of your operating system. © The Close Button— this button (X) exits the program. ‘* The Maximize/Restore Button ~ the active window can be set to occupy the entire sereen (maximized) or just a portion of the screen with this button (C1). When the window is not maximized, it can be resized with the following steps 1. Place the eursor over one of the edges or at the comer of the window until the cursor changes to a double arrowhead. Click the left mouse button and drag the cursor in or out to reduce or enlarge the window. 2 The Minimize Button —this button (_ ) reduces the program window to an icon on the task bar on the bottom of the screen. See the sample screen below to locate the Close, Maximize/Restore and Minimize buttons. Close Maximize/Restore Minimize — ‘Tonle Wiexdow Heb ra Be fet Vi BSls\<|_ ¥j/alelx| ala] ©} Users Guide27 274 ‘© Shortcut keys are indicated by an underlined character in a menu item... From the ‘Main Menu, press the key along with the shortcut key to open that menu item. While the menu is active, pressing the key associated with the underlined character is the same as clicking on that menu item. Most of the selection buttons that are used throughout this program also include shortcut keys. © Many of the windows throughout this program consist of tabbed notebooks. When the Wifidow is first opened, one page of the notebook will be active. The rest of the pages will be hidden, but their tabs will be visible atthe top or bottom of the ‘window. To change pages in these notebooks, click on the tab for the page you ‘want; that page will become the active screen. Reference for Item Naming Conventions The BioCommand Lite program uses a system of naming items that is described in this section. Once a display name is given to @ recipe or a batch, that name is used to identify all items belonging to that recipe or batch. The format for these items is: display name.Bx:item name.y where display name —__ is the name given to the recipe or batch x is the number of the batch being used item name is the functional name for the item y is the number of the instance of this item Naming Conventions FileName Function: Extension: -RCP Recipe Files (contain all information necessary to run a batch) BDB Batch Files (contain all logged data, including alarms and, where appropriate, offline data) MDW User Workgroup identification files (indicating recognized Users and their security levels) New Brunswick Scientific Users GuideTHIS PAGE INTENTIONALLY BLANK BioCommand Lite @ M1286-0050, Users Guide" 3 BIOCOMMAND LITE INSTALLATION & SET-UP This chapter guides you through initial program installation, configuration and startup. This chapter also introduces and defines the terms “recipe and “batch” For detailed system requirements, consult Chapter 12. 34 Computer Setup ‘The following subsections provide step-by-step instructions for computers running ‘Windows® 98, NT, 2000 or ME. The Windows® 2000 instructions are applicable to ‘Windows® XP and Windows® XP Pro. 3.1.1 ‘Snore: If you do not know your Product Registration Number, vi (www.nbsc.com) to register your software and to obtain on 1s Windows® 98 isit the NBS website Install BioCommand Lite: a. Start your computer in Windows® 98. b. Close all open programs, including virus detectors, desktop managers, and application toolbars. ¢. Insert the CD-ROM in the appropriate drive. 4. You will be prompted to “Please enter the Product Registration Number”. Enter the registration number and click on OK. e. Follow the on-screen instructions to run the installation process. Default values are recommended for file locations. Ifa program group window is open, close it. NBSHardwareConnection is a program that sets up communications between the computer and controller(s). Before running BioCommand Lite for the first time, or whenever attached controllers are changed, run NBSHardwareConnection as explained in Chapter 4. Start BioCommand Lite and verify communication with connected fermentors, New Brunswick Scientific User's Guide2 3.1.2 Snore: Windows NT® If you do not know your number, visit the NBS website (www.nbsc.com) to register your software and to obtain the Product Registration Number. 1 2, Make sure Windows NT® service pack 5 is installed. Install BioCommand Lite a. Start your computer in Windows NT®. b. Close all open programs, including virus detectors, desktop managers, and application toolbars, ©. Insert the CD-ROM in the appropriate drive. d. You will be prompted to “Please enter the Product Registration Number”. Enter the registration number and click on OK. e. Follow the on-screen instructions to run the installation process. Default values are recommended for file locations. If program group window is open, close it NBSHardwareConnection is a program that sets up communications between the computer and controller(s). Before running BioCommand Lite for the first time, or whenever attached controllers are changed, run NBSHardwareConnection as explained in Chapter 4 Start BioCommand Lite and verify communication with connected fermentors. Windows 2000° ‘These instructions are also applicable to Windows® XP and XP Pro. TS NOTI If you do not know your number, visit the NBS website (www.nbsc.com) to register your software and to obtain the Product Registration Number. 1 Install BioCommand Lite: a, Start your computer in Windows 2000°, b. Close all open programs, including virus detectors, desktop managers, and application toolbars. cc. Insert the CD-ROM in the appropriate drive. . You will be prompted to “Please enter the Product Registration Number”. Enter the registration number and click on OK e. Follow the on-screen instructions to run the installation process. Default values are recommended for file locations. BioCommand Lite © M1286-0050, User's Guide3 PS. notE: 3.1.4 If the program group window remains open, click anywhere outside its edges to return to the installation wizard. 2. Ifa program group window is open, close it. $¢ WBSHardwareConnection is a program that sets up communications between the computer and controller(s). Before running BioCommand Lite for the first time, of whenever attached controllers are changed, run ‘NBSHardwareConnection as explained in Chapter 4 4, Start BioCommand Lite and verify communication with connected fermentors Windows ME® SL note: 3.2 If you do not know your number, visit the NBS website (www.nbsc.com) to register your software and to obtain the Product Registration Number. 1. Install BioCommand Lite: 2. Start your computer in Windows ME*. ‘4. Close all open programs. including virus detectors, desktop managers, and application toolbars. b. Insert the CD-ROM in the appropriate drive. ¢. You will be prompted to “Please enter the Product Registration Number” Emter the registration number and click on OK 4. Follow the on-screen instructions to run the installation process, Default values are recommended for file locations. Ifa program group window is open, close it. -NBSHardwareConnection is a program that sets up communications between ‘the computer and controller(s). Before running BioCommand Lite for the first time, or whenever attached controllers are changed, run NBSHardwareConnection as explained in Chapter 4. 5, Start BioCommand Lite and verify communication with connected fermentors Be Starting BioCommand Lite “After BioCommand Lite has been successfully installed on your computer, it may be started in any of the usual ways, such as: New Brunswick Scientific Users Guide14. 3.3 34 © From the 3 Start button: Click 34 Start > Programs > NBS % BioCommand Lite From a desktop shortcut: Double click a shortcut previously placed on the desktop (refer to a Windows® manual) From the Windows Explorer®: double click on “BC32Lite.exe” in the NBS folder. Recipes and Batches ‘The terms Recipe and Batch appear throughout this manual. ‘A Recipe is a process description that BioCommand Lite uses to control a fermentation process. Recipes are created and defined by users. A Recipe is a template that dictates how the process will proceed. Recipes include initial setpoints for controlled parameters such as temperature, pH, dissolved oxygen and others. Recipes may also include programs that alter setpoints at certain times or under specified conditions. Other Recipe parameters may include display types. data log interval, alarm and limit values, types of reports to generate, and even which operators have authority to alter the recipe itself or the runs derived from it. Batch refers to a specific fermentation or cell culture run, Every Batch starts from a Recipe. The same Recipe may be used over and over to run several Batches. Once a batch has begun, Recipe dictates may be overridden. “Starting BioCommand” Screen Whenever you open BioCommand Lite, you will see the “Starting BioCommand” sereen represented below (unless you turn it off): ‘Want to rm a fermentation batch? First youneed a recipe. You can: 6 Cute arm ace wih Balch Ward | © Opn abinirce | © Openaneitngrecne ey © Ewtact arene om 8 batch You can also: — | © Renae ebatchwering hen Carmen eed TF eet saith apa on tu ‘BioCommand Lite @ M1286-0050 Users Guide45 This screen offers five options: © Create a new recipe with Batch Wizard: opens the Wizard which guides you step by step through recipe creation, © Opena blank recipe: once you are familiar with BioCommand Lite, you can use this shortcut to create a recipe, without the Wizard. Open an existing recipe: one of several ways to access existing recipes. Extract # recipe from a bateh: this use of the Batch Wizard will allow you to create a recipe used from a previous batch’'s archive file. E © Resume a batch running when BioCommand Lite exited: whether it was a power failure, a PC failure or user error, even though BioCommand Lite was not properly shut down, this command will allow you to resume computer supervision of an active batch. 3.4.4 Turning the “Starting BioCommand” Screen Off & On To prevent the “Starting BioCommand” screen from appearing in future runs, click in the white checkbox (“Don’t show this display on start up”), then close the window You can tum it back on by selecting File — Start Options from the Main Menu. The “Starting BioCommand” screen will open. Click in the box to remove the checkmark, then close the window (or continue if you wish to use the Wizard), 3.5 Main Menu ‘The menu items on the main BioCommang Lite screen comprise the Main Menu. Below the ‘Main Menu is the optional Toolbar, Many of the most commonly used functions that are available from the Main Menu or its submenus are available by clicking on the appropriate icon on this toolbar. When the cursor is placed over an icon, a one- or two-word hint describing the function of that icon is displayed for a short time in a small box beneath the icon. New Brunswick Scientific Users Guide‘This is the appearance of the Main Screen, under the “Starting BioCommand” screen, when the program is started 3.6 Main Toolbar The Toolbar may be toggled off from the View dropdown menu if more screen area for programoutput display is desired. The figure below indicates the function 7 each icon on the Toolbar. z New Batch shi P asriniee fi What's This? HELP | BjB|o|<| x [ele] x| BlS| | Cut Copy Standard Windows® functions Print BioGommand Lite © M1286-0050, Use*s Guide7 44 4414 41.2 4 NBS HARDWARE CONNECTION SETUP This setup program is required to allow your computer, BioCommand Lite, and all controllers and fermentors in your system to communicate with each other. This ‘program must be run before you attempt to use BioCommand Lite Cable Connections Before you run this set-up program, you must make sure that your hardware is properly cabled. Required Hardware The cable (#M1286-8000) included with BioCommand Lite provides communication using AFS protocol with any single NBS controller that supports RS-232 communications. This controller may be any one of the following: '* BioFlo 110 Primary Control Unit © BioFlo 4000 © BioFlo 2000 © BioFlo 5000 © BioFo Il © CelliGen Plus © BioFlo 3000 * Mobile Pilot Plant © BioFlo IV * ML-4100 controller To supervise this combination of parameters: More than one controller * Controllers that do not support RS-232 communications (such as the NBS ML-6100) you will need to add additional components: * One NBS Multi-Controller Adapter (#M1286-0100) to provide communication between the computer and the controllers © One NBS RS-422 cable (#M1171-8010) per supervised fermentor. Making Connections In this section, the term “controller” includes the BioFlo Primary Control Unit, dedicated controllers, and built-in fermentor controllers. New Brunswick Scientific Users Guide18 To use BioCommand Lite to supervise a single controller: 1. Connect cable (#M1286-8000) to link to the controller’s AFS serial port. 2. Connect the other end of the cable to the computer’s serial port. Pa NOTE: With either of the following two multiple controller set-ups, all controllers must use the same protocol. ‘i To use BioCommand Lite with AFS protocol to supervise two to eight controllers: 1. Connect an accessory NBS RS-422 cable (#M1171-8010) to the AFS serial ‘output port of one controller. 2. Connect the cable end of an accessory NBS Multi-Controller Adapter (#M1286-0100) to the NBS RS-422 cable 3. Connect the adapter end marked “RS-232" to the serial input port of the computer. 4. Make sure the controller is set to AFS protocol. Consult the controller user guide for instructions. 5. Additional controllers may be connected using additional RS-422 cables to daisy chain from the first controller to the second, and so on. BioCommand Lite also works with the NBS Interface (see interface box manual for details). 42 Setting Up Computer/Controller(s) Communication 23 note: Follow the instructions in this section carefully to ensure that BioCommand Lite communicates with your hardware. In order for BioCommand Lite to share information with each of the attached controllers, you must provide three essential pieces of information to the software: © type of conection (direct/multi-fermentor adapter or NBS BioCommand Interface) © which computer port is connected to the controller(s) © the unique “ID number” of each controller. Use the NBS Hardware Connection Setup program to enter this information. ‘BioCommand Lite M1286-0050 Users Guide419 4.24 NBS Hardware Connection Setup 1, Open NBS Hardware Connection Setup by clicking, in succession, on 2 Start > Programs > NBS > NBSHardwareConnection 2. The program window opens: sess) } | NBS BioCommand Hardware Connection Setup NS Corel ug cet caemcton ‘oth NBS afore NBS Coe vaNBS BeCermid neice is connie oss Pe Cree how the Contes coment he FC (ier btGaamand sony Kea soir ieraaton {D'tamantste mi oe comcabotel New Brunswick Scientific Users Guide20 4, Click on one option button (): © NBS Controller(s) using direct-to-PC connection or the NBS Multi- Fermentor Adapter. Choose this option if your controller :s connected either (a) via cable directly to the Comm port of your comptter, or (b) via cable to the NBS Multi-Fermentor Adapter (NBS kit number M1291- 8000), which in turn is connected to the Comm port of your computer. © NBS Controller(s) via NBS BioCommand Interface. Chcose this option if you have installed an NBS BioCommand Interface between the fermentor(s) and the Comm port of your computer. ‘The NBS Multi-Fermentor Adapter and the NBS BioCommand Interface are optional accessories that allow the use of up to eight controllers with your software. ‘Snore: Anytime you change connection type, to protect you from operator error, the following warning screen will open: CE A\, Yous saute change te det conection ypo used by BicConmane. Oroe ths change i mace, FeConmard ? DA a Rae tempts mach conrartsotasuang tne Ube of comacton Go ahead and mak ts cranga? [yes] Click on {Yes} to apply the change or {No} to cancel Click on (Next >>} to open the Computer Communications window (see Section 4.2.2), 4.2.2 Select Comm Port 1. Inthe “Select Comm Port” pane, indicate which computer Comm port, 1 or 2, is connected to your controller(s) BioCommand Lite @ M1286-0060, Users Guide4.23 Click here to change the number \— 9600 is the jeereiie only choice here. 2. Inthe “Comm Settings” pane, the correct choices are preset. There are no user-selectable items in this group of settings. 3. Click on the {Open Comm Port} button to test the selected Comm port. 4, Ifyou get an error message. it most likely means you have incorrectly identified the port connected to your controller(s). Change the “Select Comm Port” setting. then click again on the {Open Comm Port} button, When the test message indicates no error, click {Next>>} to open the Controller ID List window (see Section 4.2.3). Controller ID List In the Controller ID List, you may enter up to eight “ID” (identification) numbers for the controller(s) attached to your computer. ID numbers are electronic “names” that allow the sofiware to communicate individually with each of the attached controllers. New Brunswick Scientific, Users Guide‘Be Heb Controller 1D List Enter the 1D numbers of nowky-eltachod controllers) SA note: « ID numbers have nothing to do with product, model or serial numbers. ¢ An NBS BioCommand Interface or Multi-Fermentor Adapter Is required to use more than one controller with BioCommand Lite. Refer to your fermentor, bioreactor or controller User Guide to learn how to view ‘the ID number for that specific instrument (see Sample Retrieval of ID Numbers on the following page). In some older NBS controllers, ID numbers are referred to. as “MD" or “multidrop” numbers. Sample Retrieval of ID Numbers To obtain the ID numbers for the BioFlo 110, for example: 1. Press the upper right BioFlo 110 touchpad key to display the Setup Screen’ BioGommand Lite M1286-0060 Users Guide2: English NBS BioFlo 110 Franpals Version : 0001.01 Config: 1.10 Deutsch @) Date: 2/2000 Espajfiol ~~ hto:www.nbso.com er] Lanna ‘SELECTOR BUTTONS 2. Press the More... selector button to display the Systems Deual screen: System Details Unit Base Address: 0 BaseAdd Unit 41D: 0 Unit 21D: 4 | Unit 31D: 2 Unit 41D: 3 Comm. Protocol: AFS Comm Recorder Status: Off Recorder 3. Note the four Unit ID number(s). 4, Ifyou have only one vessel, use only the first ID number. Enter that number in field | of the Controller ID List. 5. If you have more than one vessel. enter additional numbers in subsequent fields of the Controller ID List. End of Sample Retrieval. New Brunswick Scientific Users Guide24 ‘When you have entered the ID numbers in the Controller ID List, click on {Next>>}. Within a few moments, the Controller Status window opens. 42.4 Controller Status ‘The Controller Status window indicates the ON/OFF status and available loops in each controller, - 1D Loops Statue Loop Lat Glick, here to FPF fe | check [Teme | entire loop lists 2S. NOTE: If the indicated status does not agree with what is observed at the controller(s), click {<< Back}, confirm the ID numbers, then click {Next >>} to return to this screen and recheck the status. If the status is still not correct, check all cable connections, and confirm the operation of each controller. When you have confirmed the correct controller status, click {Finish} to exit NBS Hardware Connection. BioCommand Lite © M1286-0050 Users Guide25 5 CREATING A RECIPE & STARTING A BATCH Follow the steps in this chapter to create a new Recipe (with or without the help of Batch Wizard), start a Batch from that Recipe, and view the Batch status using a Batch Summary display. The Recipe, Batch, and Display in this chapter are “minimalist” in that they do not invoke the most powerful or impressive BioCommand Lite capabilities. Nevertheless, performing the steps in this tutorial will impart a feel to the new user for how the software works and what it does. To start a Batch you must either specify a previously saved Recipe or create a new one. A Recipe must contain at least the following information: A data recording interval A data log file name A list of parameters (loops) identified by name and the controller in which they reside Recipes usually contain more than the minimum required information. The Recipe created in this section is minimal, but it will be expanded in later chapters. The easiest way to create a recipe is to use the Batch Wizard; other methods are discussed in Section 5.3 54 Creating a New Recipe using Batch Wizard In the “Starting BioCommand” screen (see Section 3.4): 1. Click on the white option button next to Create a new recipe with Batch Wizard (if it is not already selected) 2. Click on {Continue} or, if you change your mind, Click on {Close} to exit Batch Wizard. New Brunswick Scientific Users Guide26 If you choose {Continue}, the “BioCommand Batch Wizard Introduction” screen opens: BioCommand Batch Wizard Invoduetion Notice, too, that the New Recipe Setup screen will also open behind it. You will have access to the Setup screens when you have finished Batch Wizard, If you do not wish to see this introduction screen when you use Batch Wizard again, click in the white checkbox next to Skip this screen in the future ‘You may use any of the active buttons at the bottom of the screen to obtain help, to cancel use of the wizard, to move back to the previous screen ot to move ahead to the next, Ifa button is gray, itis inactive for that screen. 3. Click on {Next>} to move ahead. The “BioCommand Batch Wizard - Step 1” screen will appear: BioGommand Lite M1286-0050 Users Guide2 The default name for the recipe, which appears in the edit box, is Recipel 4. Ifyou wish to keep this name, simply click on {Next>}. This is the name that will appear on the bottom Recipe tab in the Setup view when you finish Batch Wizard. It will also appear in the title bar of all windows associated with this recipe. 5. If you wish to rename the recipe, enter the new name in the edit box. You may use up to 12 alphanumeric characters, including spaces but no punctuation marks. Then click on {Nex}. The “BioCommand Batch Wizard ~ Step 2" sereen will appear: PieCommand Batch Wicaid St Sie sect the hardnreoops obe raed in ths ‘oops There logs canbe frm specs ant recy ‘ecto ts tr Yousan browse for hardvart ove therein New Brunswick Scientific Users Guide6. Click on the {Select Loops>>} button, ‘The “Add/Change Hardware Loop List” window will appear: I ore “toons InBetch Lilien fos 7. Click on the {Browse Local Server} button, Wait a moment while BioCommand Lite searches. The available controller(s) will appear in the left “Available Loops” pane. 8. Click on the + next to the controller(s) of interest to see the drop-down list of available loops. 9. If you wish to select all loops from a controller, click on the controller name, then click on the {>>} button. The controller name (and all its associated loops) will move into the right, “Loops in Batch” pane. 10. If you wish to select one loop at a time, click on the loop name, then click on the {>>} button. The loop name will move into the “Loops in Batch” pane. 11. When your selections are complete, click on {Close}. You will return to the Step 2 Batch Wizard screen. BioGommand Lite @ M1286-0050 Users Guide29 12. Click on {Next>}. The “BioCommand Batch Wizard — Step 3” screen will appear: ‘You must assign a ‘Recipe File Name’, which is both a file name and its folder location, Note that every recipe must be assigned the extension RCP that marks it as a recipe. 13. Click on the {Change Directory/Name >>} button. 14. The “BioCommand Save Files” window will open: 15. To see existing File Name/Folder strings, click on the down arrow (~ ) to the right of the ‘File’ edit box. 16. Click on an existing File Name/Folder string to bring it into the edit box as a patter. You can type over it to create your new name, This will not affect the existing File Name in any way. New Brunswick Scientific Users Guide17. If you wish to find other folders, click on the {Browse} button. The screen that appears will allow you to explore possibilities (in network if shared, or in your own PC ifnot), just as you would with any other Windows® -based exploration tool 18, Create anew one based on this example. Be sure to follow the pattern: Drive:\FolderName\FileName.RCP (the example shown on the Step 3 screen above is CABC32\BioRep2.RCP). Normal Windows® file-naming conventions apply 2S. NOTE: All recipe file names must have the extension “.RCP”. Click on {OK}. Your selection will move into the ‘Recipe File name” edit box. 19. Click on {Next>}. The “BioCommand Batch Wizard — Step 4” screen will appear: 20. After you have read this screen, click on {Next>}. The “BioCommand Batch Wizard — Finished!” screen will appear: BioCommand Lite @ M1286-0050, Use"s Guide21. Click on {Finish}, You will now be in the Setup View for the Recipe you have just created. ‘At this point, you have a very basie recipe that is sufficient to start a batch. If you are ready 10 do so, see Section 5.4, Starting a Batch, for further instructions. If you wish to add any or all of the additional features mentioned in Step 4 of the Batch Wizard, see Section 5.2, Expanding a Basic Recipe, below. 5.2 Expanding a Basic Recipe At any time (before you start a batch or while a batch is running), you can expand a recipe using any or all of the features below, which are also mentioned in Step 4 of the Batch Wizard: © Change the logging interval See Section 10.1.4, Time Data Pane ‘* Set the Elapsed Fermentation Time format See Section 10.1.4, Time Data Pane ‘© Add Alarm settings See Chapter 7 ‘© Select parameters for trend views See Section 6.2 © Modify the hardware loop list See Sections 5.3.2 and 10.1.5 New Brunswick Scientific Users Guide5.3 Alternative Methods to Create a New Recipe There are a number of ways to create a recipe. The easiest is to use the Betch Wizard, as outlined in Section 5.1. You can also create the recipe without the Wizaré, directly from the “New Recipe Startup” screen. There are several ways to access the Wizard, and several other ways to access the “New Recipe Startup” screen. 5.3.4 How to Access Batch Wizard : There are two ways to open the Batch Wizard: ¢ On sstart-up, from the “Starting BioCommand” screen, cl recipe with Batch Wizard From the Main menu, click on the Batch Wizard icon. 5.3.2 How to Access the New Recipe Startup Screen There are three ways to open the “New Recipe Startup” screen: + From the “Starting BioCommand” screen , click on Open a blank recipe, then click on {Continue} ‘Want to run fermentation batch? ‘First you needarrecipe, pYou-can: Croat a naw recipe with Batch Wea © Open abiark coe © Open an exiting recipe 1 © Sitat wes toma batch | You cen also: ——————. (© Resume abarch uring when BicConmand ested T Dont show bis day on sat up © Select File - New Recipe from the Main Menu. © Click on the New Recipe icon on the toolbar. BioGommand Lite @ 71286-0060 Users Guide33 After any of the above actions, the “New Recipe Setup” screen will appear: rele | Dip ate | Dereon 2 Se TineDela | Reopen nae i cal See 1. Click in the ‘Display (Tab) Name:’ edit box and type the display name for this recipe. The name may be any combination of up to 12 alphanumeric characters and spaces 1k inside the ‘Description:" edit box and enter descriptive text as desired. This Description may be any combination of alphanumeries, spaces, and punctuation marks. The default Log Interval is one minute. To change the interval, click the {Change Interval} button in the “Time Data’ pane. Enter the desired Log Interval in whole minutes in the edit box, or choose a preset interval from the dropdown list, 4, Select the Time Display Format, either HH:MM-SS (Hours : Minutes : Seconds) or Decimal Hours, by clicking in the appropriate circle. Decimal format is convenient for mathematical processing. New Brunswick Scientific Users GuideLogintval Mie) CuretF Loe 5. Click on the “Loops” tab, The “Add/Change Hardware Loop List” window appears, This window allows you to select loops from any of the attached controllers. Usually all the loops from a single fermentor are selected. You may choose to include loops from ancillary equipment, such as a gas analyzer or scale. 2 | Avabable Leone: Loaps nate 4 BioGommand Lite M1286-0050, Users Guide35 6. Click the {Browse Local Server} button. Controllers that are accessible to BioCommand Lite appear in the field labeled “Available Loops”. In the sample screen below, loops from two local controllers are available for this recipe: 7. To display the individual loops associated with each controller, click on the “+” box to the left of the controller name. The list expands as shown below: 8. Pressing the {>>} button moves selected loops from the “Available Loops” pane to the “Loops in Batch” pane. Click on a controller name and then click on the{>>} button to include all the loops from that controller in your recipe, or select an individual loop from the expanded list and click on the {>>} button to include that loop in your recipe. Continue until all desired loops are in the right pane of this window. New Brunswick Scientific Users Guide36. 9. Use the {<<} button to move loops from the “Loops in Batch:” list back to the back to the “Available Loops” list, if necessary. 10, When all the desired loops are in the “Loops in Batch:” list, click {Close}. The Loops page of the Setup View reappears FIASCO FEWERSON\Cosie 244A [EMERSON Cemetec4s\002 9) [EMERSONS Cermleces\Foan |) (EMERSON Geode 4s [EMERSONSCaroteces 4) [EMERSONSCarslatAres 1) [EwERSONSCarrota4s\ oop 11. The “Current Loops” pane shows the selected loops. On the left of each line is the loop name as transmitted to BioCommand Lite by the controller. On the right is complete loop data in the format [Server Name\Controller Name\Loop Name]. BioCommand Lite M1286-0060 Users Guide37 You may change any default loop name to a display name containing a maximum of 10 characters. You may also enter an initial setpoint for each loop in the Recipe. Both of these options are executed through the “Loop Setup Options” window. 12. Click on the {View loop details} button. The Loop Setup Options window will open: I toes: fag _ EMENSONS\Cowods 7p 7 Sioa A. Select the loop from the pull-down menu. B. Enter a new display name, if desired. C. Enter a setpoint, if desired. D. Click {Apply}. E. Repeat these last four steps for each loop to be changed 13. Click {OK} to retum to the Loops tab of the “New Recipe Setup” window The Recipe is now sufficiently complete to start a Batch. Many other Recipe functions are available, but they are not necessary to start a Batch. They will be covered in later sections (see Section 5.2 for a cross-reference guide of the most popular features), Before you start a Batch, however, you need to save the Recipe: 1. Click {Save}. ‘The “BioCommand Save Files” window will open 2. Enter a file name in the File edit box, identifying the drive, folder, name, and extension. Click on the down arrow for sample file names (note that the File extension must be .RCP and that your default recipe folder is BC32). A typical file name might be CABC32\Recipel.RCP. Click {OK}. The “New Recipe Setup” screen re-appears. 4. Click the {Save} button. At this point you can either close the Recipe window or start a Batch from this recipe. 5. If you opt to close, the “Save Changes” window will open. Click on {Yes} New Brunswick Scientiic, Users Guide5A 5.4.1 Starting A Batch Before starting a Batch, prepare the fermentor. Be certain that these steps have been taken: ‘The fermentor and controller are powered on. There is media in the vessel. AlFappropriate liquid and/or gas addition systems are attached to vessel The vessel, media and liquid addition systems have been sterilized as required The probes have been calibrated. All ancillary equipment has been connected, powered on and tested. Any other procedures that need to be performed prior to starting a culture should be completed before starting computer supervision and control. ‘Most users prefer to start the Batch in BioCommand Lite before inoculating, Doing so sufficiently in advance establishes correct growth conditions according to the Recipe, and enables confirmation of data logging and other functions before growth begins. The Elapsed Fermentation Time clock can be reset to zero when inoculation or any other biologically significant event occurs. Selecting a Recipe To start a Batch, you first need a Recipe. There are several ways to select the desired recipe: (1) “Starting BioCommand” Screen A. Click on Open an existing recipe (see screen below): i (oid | Want to runa fermentation batch? Firstyounced a recipe. You can: ———— ae | © Cmte reps th Beh Wiad | © Gam ebienctecpe | [© Beeranesing reco BioCommand Lite © M1286-0050 Users Guide39 B. Click on {Continue}. The “Select File to Open” screen will appear (see sample screen below). You will also notice the “View Recipe Setup” screen shadowed behind it. joke [See da ies) __ ere eects elite Tel Recoste Stee [picrccsie — lalizep [Bliecpe5 6 BiResOreo a BioRenSiep sf Recetiep Sal Renesas [eB0Rest ep deco liecipeico Ea} Recbere: BoRep2 rep leicoakinsep i Racinediep a) Revs PeleoAcearcp §— (SYEVRee2co—ieceetiep lal Receows il ee ay a a + edie FRR ————S] oe Click on the recipe of interest. Click on {Open}. The “Select File to Open” screen will disappear, and your selected recipe will be displayed in the “View Recipe Setup” screen. You will be able to verity the recipe parameters by cycling through the tabs. If you wish to modify the recipe, see Section 10.1 for more information. E. Continue in Section 5.4.3 below. co (2) Main Screen File Menu ~ New Batch Click on File, then New Batch from the dropdown menu, The “Select File to Open” sereen will open (see sample above), with the “New Recipe Setup” screen shadowed behind (3) Main Screen Toolbar - New Batch Icon Click on the New Batch Icon, The “Select File to Open” screen will appear (see sample above), with the “New Recipe Setup” screen shadowed behind it. (4) Use “Starting BioCommand” Screen to Extract Recipe This option is handy if you know the Batch File Name but not the Recipe File Name. New Brunswick Scientific User's Guidea 1 ‘Want to rama fermentation batch? First you needa recipe. oleae re a \ © Create a new recipe with Batch Wizard | Sortirae | © oometlekicee | © Open an existing recipe: | a 6 Eaiesaiaee Taal | You can also: — © Bona abacheercantenSeczementess | 7 Dont show hie ea en tt up A. Click on Extract a recipe from a batch and then click on {Continue} The “Select File to Open” screen will appear, with the “View Recipe G Setup” screen shadowed behind it. Note that this “Select File to Open” screen will show all available Batch files (*.BDB), instead of recipe files (*.RCP). See sample screen below: fa) ‘al Specittbeb Recpeliods x} Speoaa bb Recpetb bch a] SpecaBa db namie bal Specatichdb [Slsencle3 bob) Seecssancb pect acta fh Fem [se] Pesce FRoeRRCaT —————S] cae Select the Batch file of interest, then click on {Open}. The “View Recipe Setup” screen will open, displaying the parameters) from the selected batch, recipe (and its D. Verify the parameters by cycling through the tabs of this screen. Now that you have selected a Recipe, if you wish to modify parameters for the new batch, you may do so at this time. See Section 10.1 for more information, Note that changing parameters for a Batch does not alter the Recipe file BioCommand Lite M1286-0050 Users Guidea 5.4.2 Deleting a Recipe or Batch ‘You can use Windows® Explorer to delete a recipe or a batch record, 5.4.3 Starting the Batch from a New Recipe “he Click the {Start Batch} button, - 2. The “Batch Display Name” screen appears: TsachOiite Nom Roce ont Chenge? FRecpetaat ox. 3. Choose a Batch display name (or accept the one already assigned) and click fOK}. 4. The “BioCommand Save Files” window (isha ox 5. To see existing File Name/Folder strings, click on the down arrow (+ ) to the right of the ‘File’ edit box or click {Browse}. 6. Click on an existing File Name/Folder string to bring it into the edit box as a pattern. Type over it to create a unique name for the Batch datafile. This will not affect the existing File Name in any way. 2S. NOTE: All batch file names must have the extension “.BDB”. 7. Click {OK}. New Brunswick Scientific, Users Guide42 544 _Fermentation Time) clock running in the Setup screen, You will see two fleeting screens: “Setting permissions...” and “Connecting to hardware...” as the software prepares to launch the new batch. A batch tab with the suffix letter “B” and the selected Batch Display Name appears at the bottom of the BioCommand Lite screen, Use this tab to select which Batch to view when several are running. The batch is now running, as indicated by the ‘Current EFT’ (Elapsed Starting the Batch from an Existing Recipe When you start a new batch from an existing recipe without changing any parameters: 1. Click the {Start Batch} button, 2. The “Save Changes” screen appears, even though you have made none: QD) sevechogstoReces? Click {No}. The “Batch Display Name” screen appears: 4. Choose a Batch display name (or accept the one already assigned) and click {OK} ‘The “BioCommand Save Files” window opens: BioCommand Lite @ 1286-0050, Users Guide6. To see existing File Name/Folder strings, click on the down arrow (+) to the right of the ‘File’ edit box or click {Browse}. 7. Click on an existing File Name/Folder string to bring it into the edit box as a pattern. Type over itto create a unique name for the Batch datafile. This will not affect the existing File Name in any way. &._Click {OK}. 2S. NOTE: 54S All batch names must have the extension “.BDB”. You will see two fleeting screens: “Setting permissions...” and “Connecting to hardware...” as the software prepares to launch the new batch, A batch tab with the suffix letter “B” and the selected Batch Display Name appears at the bottom of the BioCommand Lite screen. Use this tab to select which Batch to view when several are running. The batch is now running, as indicated by the ‘Current EFT’ (Elapsed Fermentation Time) clock running in the Setup screen. Starting the Batch from an Existing Recipe with Changes When you start a new batch from an existing recipe and you change any of the recipe’s parameters: 1. Click the {Start Batch} button 2. Ifyou saved the changes before you clicked {Start Batch}, skip to Step 3 If you did not save the changes while you were in the Recipe Setup screen, the “Save Changes” screen appears. Click {Yes} (2D) seve chegee To Recon? New Brunswick Scientific, Users Guide3 4, Choose a Batch display name (or accept the one already assigned) and click {OK} 5. The ‘ommand Save Files” window opens: CE 6. To see existing File Name/Folder strings, click on the down arrow (~ ) to the right of the “File” edit box or click {Browse}, 7. Click on an existing File Name/Folder string to bring it into the edit box as a pattern, Type over it to create a unique name for the Batch datafile. This will not affect the existing File Name in any way. 8. Click {OK}. 2S. Note: All batch file names must have the extension “.BDB”. ‘You will see two fleeting screens: “Setting permissions...” and “Connecting to hardware...” as the software prepares to launch the new batch. A batch tab with the suffix letter “B” and the selected Batch Display Name appears at the bottom of the BioCommand Lite screen. Use this tab to select which Batch to view when several are running. The batch is now running, as indicated by the ‘Current EFT” (Elapsed Fermentation Time) clock running in the Setup screen. BioCommand Lite @ M1286-00506 BATCH VIEWS In BioCommand Lite, the Batch Summary display and Trend Views offer tools to automatically chart the activity of your batch. Master the easy BioCommand Lite display tools, and you can create informative, convenient displays. 6.1 Batch Summary View ‘You can view a text summary of current Batch status by calling up the Batch Summary View. This is a tabular list of the loops that are active for the Batch being run, showing the current value, setpoint, and output levels for each. ‘The Batch Summary view is available by default in all recipes. It is automatically set up by BioCommand Lite. Its layout, function or content cannot be changed by the user. ‘There are several ways to display the Batch Summary View: (1) From the Setup window, click on the Batch name tab at the bottom of the BioCommand Lite screen. (2) From the Main Menu, select View — Batch Summary. (3) From the Setup window, click on the Views tab, then double click on Batch Summary in the ‘Current Views’ pane. The Batch Summary view will open: eA CMe [SP OU[Mode 350, PLD. 50 PAD TO7622| PD New Brunswick Scientific, Users GuideBatch Summary View Column Headings Column Heading Description Loop Loop Name cv Current Value offoop Units Measuring Units for Current Value ‘SP Loop Setpoint fou ‘Controller Output for loop [Mode ‘Control Mode for loop 6.2 Trend Views : Many times it is most desirable to view the data plotted as a scientific graph in real-time A Trend View is a line graph of fermentation data plotted against time. The program allows you to select the data items to plot on the graph, the scale values to use, and other parameters regarding the plot. You may select up to eight different data items to plot in real time while the Batch is running. Each data item is displayed in a unique color so that the plots may be easily distinguished from each other. AA different vertical scale may be assigned to each plot. ‘You can create multiple Trend Views to display more than eight loop items. 6.2.4 Creating a Trend View ‘You can create a Trend View in the Recipe Setup window, or you can add one to the Batch view after the Batch is running. To create a simple Trend View: 1. From the main menu, select File-View Recipe, or click on the View Recipe icon. Select the Recipe of choice. From the recipe Setup window, select the Views tab (see sample screen below): | Joe = BioCommand Lite © M1266-0050 Users Guide47 4, Double-click on Trend in the ‘New Views:” pane to open the Trend window, which will look like the sample screen below: | PLOT AREA: CONTROL PANEL: see Sections 6.2.3- 6.2.6 for details. 5. Select the “Loops” tab in the control panel at the bottom of the window. This, changes the control panel to the ControlLoop selection screen, as illustrated below: 6. Click on the {Setup Loop! } button to open the “Select Loo} list. These are the available loops that have been specified for this recipe ott oe: Foun’ = + wo Pet | Teno New Brunswick Scientific User's Guide7. Click on the loop that is desired for the first plot and then click the {OK} button. This will add the loop name to the ‘ControlLoop’ window. Click on the {>>} button to change the control panel button to {Setup Loop2}. This procedure may be repeated for as many as eight loops. 8. Select the “Graphs” tab on the control panel for the Trend View. The control panel will change to a screen as shown below. This allows you to set the high and low limits on the y-axis for each data item that is to be plotted. Normal Windows® editing techniques will allow any of the numerical parameters to be changed. You may also edit the Start and End times for the Trend View. Note, however, that changing either time value will change the value for all parameters plotted 9. Click on the {>>} and {<<} buttons to step through the selected loops. 10, Click on the “Styles” tab on the control panel to open a window, as shown below, that allows the line and plot style for each loop to be set. ‘The default for each plot is a point-to-point thin line. This may >e changed to a thicker line and to any of the styles shown in the view above. To change the style: A. Click on the option button next to the desired style and thickness. B. The {<<} and {>>} buttons may be used to step through the loops to set the parameters for each. C. Click on any other tab to save your changes. This completes the design of this simple Trend View. To save it; BioCommand Lite @ M1286-0050 Users Guide49 1. Right click with the cursor anywhere in the gray area of the lower screen (on either side of the control panel) 2. Select Save View from the dropdown menu to save the Trend View you have created. This will add the view to the Recipe 3. The Trend View, automatically labeled by BioCommand Lite as Trend.1, Trend.2, ete., will appear in the ‘Current Views’ pane the next time you return to the “Views” tab. ee 6.22 If the trend graph was entered as part of the New Recipe or View Recipe function, the trend graph will be available to all batches ini recipe. ited from the If it was entered from a Batch function, then the trend graph will be available only in the present Batch. Using the Trend Graph The Trend View window is shown below: ¢ The Plot Area is the region where the data is plotted © There are two y-axis scales visible at any time. Their scale may be changed to be appropriate for the various loop values that you select for plotting. See Section 6.2.5 for details. © The x-axis isa time axis for the Elapsed Fermentation Time (EFT). It initially starts at zero and is one hour wide. The time scale may be altered to whatever you feel is appropriate. This will be explained later in this section. New Brunswick Scientific Users Guide© Atthe bottom of the window is the control panel that is used to set up and modify the plot, and to display the Current Values for the loops being plotted (In the sample screen, some of the values are erroneous because this is a virtual batch, not a real one.) RIGHT Y-axis: ‘Sealed & colored for Loop LEFT Y-AXIS: Scaled & colored for Agitation Loop ‘EE PLOT AREA XAXIS: = XAXIS: end rarer ofp FT plot ert © } contro change pot Panel ne oe ‘CURRENT VALUE readout (loop identined by color) © Each of the notebook tabs on this control panel is described separately below. When the Trend View is onscreen, the “Main” tab should be selected on the control panel. This will present a screen on the control panel with eight display windows on it. Each window, as illustrated in the sample screen below, will display a loop name in the color of the plot, as long as the cursor is outside the plot area. If the cursor is moved inside the plot area (i.e., onto the graph), each of these windows will display the current value for the loop, as illustrated below: BioCommand Lite @ M1286-0050 Users Guide54 t PLOT AREA Beteeee rE ‘rent Tere If the Batch is not running, these values will all be 0 (as indicated in the sample screen above) When the view is first entered, the left y-axis will display the name, color and scale for Loop!, and the right y-axis will be named, colored and scaled for Loop2. Clicking on the loop name on the control panel “Main” tab will shift these labels. With each click the new loop label will replace the current label, first on the left side, then the right side. It will alternate with each succeeding click. You can change the Trend View to show a larger graph area by clicking anywhere on the gray’ areas of the view. The control panel will reduce to colored buttons, each representing its assigned loop, as show below. ‘rete ef EERE PEECEPEEEEE New Brunswick Scientific User's GuideWhen you click on a loop button, one of the two y-axes will be assigned to that loop. Each additional click, on that button or another one, will alternate from the left side to the right side of the graph. At any time you can click again in the lower gray area of the window to return to the main Trend View (with the control panel). While you are in the colored block view (see sample above), if you click on the small, white triangle at the lower right comer of the view, the display will ‘Change. Now it will show only the two loops identified by thé y-axes on the left and right sides of the graph, f ‘When your cursor is outside the plot area, two small panes separated by the EFT time clock will identify the loops they represent. Note that each Icop name is written in the color you assigned to it (see sample screen below). ‘a i . If the cursor is inside the plot area, the panel will show the current values of the two loops as shown in the sample screen below BioCommand Lite M1286-0060 Users Guide3 i You can use the slider bar (just below the x-axis) to move the graph display through the Batch run time record. Place the cursor on the slider, hold the left ‘mouse button down, and move the mouse horizontally right or left. The plot will move forward or backward through the data, You can also use the arrow keys (4 of >) to move through the time record. For convenience, at any time you can click on the far left clock readout to return to the 00:00:00 start time, You can also scroll through the graph using the cursor. As your cursor moves horizontally within the plot area, click once and hold it. rolling vertical plot line will appear under the point of your cursor. The time and current values will read out as you move the plot line. For easy reference, double click to leave plot lines at desired intervals. If you right-click the mouse while your cursor is on the Trend View window but outside the Control Panel , a submenu with five choices will appear (as shown below): SEESERES ER? 3 5 4 3 gOTyrTerses New Brunswick Scientific Users Guide6.2.3 6.24 ‘The fianctions of these selections are: © Default View ~ selects this view as the Default view. ‘Save View ~ updates the Recipe or Batch file by saving the current view. ‘¢ Delete View — removes this view ftom the Batch or Recipe thet is active at the time. © Print View — prints the view on the default printer connected to the system. Zoom — allows the time scale of the plot to be changed to 2 greater (“Out”) value or lesser (“In”) value. . At any time, click again in the gray area to return to the tabbed control panel. MAIN Control Panel Tab When a batch is active, the loop panes in this control panel will display the Current Value for each loop, as long as your cursor is somewhere inside the plot area, In the sample screen above, all panes display zeroes because no batch is active. Note that the each value is shown in its loop’s plot color. When your cursor is outside the plot area, the loop panes display the loop name, each in its plot color. LOOPS Control Panel Tab Selecting the “Loops” tab from the Control Panel changes the display to the window shown below Use this window to assign colors and plot order (1 to 8) to the loops you will be plotting in this view. 1. Click on the {Setup Loop!} button. The “Select Loop” window will open. BioCommand Lite @ M71286-0050 Users Guide55 2. Select the name of the loop you want to see plotted first, and click on {OK} in the “Select Loop” window. The loop name will appear in the “ControlLoop’ box, 3. Click on the {>>} button to move to the next loop. In a new color, the button will now be labeled Setup Loop2. 4, Repeat the above steps until all loops have been assigned a number and color. 6.2.5 GRAPHS Control Panel Tab Once the loops have been selected for plotting, it is appropriate to set the plot scales for each, 1. Click on the Graphs tab: Loop number you have assigned Loop name you have assigned, chosen color ‘You will automatically be given Loop | to work with. Set the low and high limits of the loop’s y-axis scale by entering numbers in the ‘Low’ and ‘High’ edit boxes. Click on the {<<} or {>} buttons to step through each loop, and to set scale limits for each one individually. As each loop number is selected, the box on the right of the pane will display the name of that loop. Each time you click on {<<} or {>>}, you save the settings you have just made for the previous loop. Moving from the Graphs tab to another tab will also save your settings. 4, You may also set the x-axis limits here: ‘View Start’ and *View End’. The default values are 00:00 to 01:00. 6.2.6 STYLES Control Panel Tab You may choose the line width and plot style for each loop in the graph from the “Styles” tab selection: New Brunswick Scientific, Users Guide6.2.7 “A. There are two line widths available. The thinner line is thie default selection: you will see that its option button is selected. If you wish to change to the thicker line, click on its option button, 2. Four plot styles are available. Click the option button of the style you choose. (The seamless point to point graph line is the default choice.) Modi g a Trend View 1. Return to the Main tab on the Control Panel. 2. Right-click with the cursor over any active loop button to open a submenu of four items as shown below: ‘The functions of these selections are: Graph Settings — this item is not curently functional Change Graph Color — allows you to change the plot color for the selected loop and the background color for the entire Trend graph. See details below. © Delete Graph — removes the selected loop from the graph BioCommand Lite @ M1286-0050 Users Guide87 Zoom — allows the time scale of the plot to be changed to a greater (“Out”) value or lesser (‘“In”) value. ‘When you select “Change Graph Color” from the above submenu, the following window opens: Pe Choose the desired ‘option: Line or —— Background ‘are going to change. Sliders to ‘custom mix a color Palette of pa ‘Suroce icp ne solor ‘colors. ‘background color. Selected loop: you are changing colors for the plotting of this loop. ‘The button-like pane in the lower right comer of the window displays the name of the loop selected for change. 1. Decide whether you wish to change the plot line color or the plot background color. 2. Click on the appropriate option button: Set Line Color or Set Background Color. 3. The pane below the Set Line Color option button displays the color that will be changed: in the sample screen, it is red. If we had selected Set Background Color, this pane would be green, as itis in our sample graphs. 4. To change the color, click on the desired color in the palette. If you do not see the color you want, you can custom mix one, Each of the three slider bars will modify the percentage of one of the three primary colors that will make up your color: New Brunswick Scientific Users Guide© The top bar controls the amount of red, the middle controls the green, and the bottom is for blue. Change the proportions of each color by moving each slider bar to the left (less) or right (more). © The left and right pointing arrows (4 and») on the ends of the slider bar may also be clicked to move the slider bar in small increments across the range. 6 The pane below the slider bars displays the current color of both background and loop plot. As you make changes, the new colors) will be cisplayed in this pane. The {Cancel} button will remove the last changes you applied. ‘The {Default} button will return the colors to the original colors defined by the program. 7. Click the {Apply} button to save and to make effective any color changes you have made. As you make color changes, they are also displayed on the ‘Trend View that is on the Main Screen after you click the {Apply} button S\NoTE: You can change the plot background color via any loop’s “Change Graph Color” screen. Of course, that new background color will apply to the plot graph for all of the loops. 8. Once you have applied your new colors, click the {Close} button to exit this function. BioCommand Lite @ M1286-0050 Users Guide58 B ALARMS BioCommand Lite allows you to enter a set of high and low limits for any or all of the loops in the Recipe. These “Alarm limits inform you when 4 loop value has passed into a dangerous range. When a value enters an alarm range, the display tab at the bottom of the screen turns red. There is also an easily accessible Alarm Summary report that displays any activity beyond the limits you set. 71 Setting Alarms 1, From a Recipe or Batch Setup screen, click on the “Alarms” tab. 2S. Note: First you must select the loops for the Recipe. These settings may be made as part of the Recipe or added to a Batch while it is running. eT io Ifa batch Is running, this button will be labeled End Batch New Brunswick Scientific User's GuideTo set an alarm for this Recipe: 2. In the first row of the ‘Loop Name’ column, click on the down arrow (¥). ‘A submenu will open with a list of the loops available for this Recipe. If the down arrow is not present, click inside the cell to make it appear. Double-click on the desired loop. That loop name will then appearin the “Loop Name’ box. Click, in the same row, the box immediately to the right of the loop name, under ‘Alarm Type’. A down arrow (¥) will appear at the right side of the box. Click the down arrow to open submenu of two selections for the alarm trigger: Absolute or Setpoint Deviation, © Double-click on Absolute to generate an alarm if the actual Current Value for the selected loop exceeds or drops below the absolute limit parameters you set, independent of Setpoint. © Double-click on Setpoint Deviation to generate an alarm if the actual Current Value for the selected Joop exceeds or drops below the setpoint tolerance you set. The submenu will close and the alarm type you have chosen will appear in the ‘Alarm Type’ box: BioGommand Lite M1286-0050 Users Guide61 6. Next, enter, in the appropriate edit boxes, the numeric value for the Low (‘Lo’) Limit and the High (‘Hi’) Limit that will govern the alarm function: New Brunswick Scientific Users Guide62 The availability of the alarm function for each loop may be turned on or off independently at any time: 1. In the row corresponding to the desired loop, click under the ‘On/Off heading, A down arrow (¥) will appear to the right. 2. Click on the down arrow to open the submenu: 3. Double-click ‘On’ to activate the alarm, or ‘Off to deactivate it. The selected action will appear in the box. 4. Click in the ‘Audible On’ checkbox if you want an alarm sound to be generated when an alarm is triggered. ‘CSNOTE: A sound card is required to use the Audible option. 3. Repeat the same steps for as many loops as you wish. 6. Click on the {Apply Changes} button, Your alarm settings for the selected loop(s) are now part of the Recipe or Batch. BioCommand Lite © M1286-0050, Users Guide63 The sample window below shows alarms set for five loops, with the Audible option enabled: New Brunswick Scientific Users GuideTHIS PAGE INTENTIONALLY BLANK BioCommand Lite @ M1286-0050, Users Guide8 ENDING A BATCH After a fermentation run has ended, and before exiting the BioCommand ___ Lite program, perform an End of Batch procedure to close the data log file and to terminate Batch supervision. Otherwise, exiting BioCommand Lite will be interpreted as an interruption, and the program will attempt to resume data logging and control of the same Batch the next time the program is started. 81 End a Batch 1, Open the Setup View. 2. Click on the {End Batch} button. A window called “End Batch?” will open, asking for confirmation that you do indeed wish to end the Batch. If you wish to terminate the Batch, click on {OK}. If you have made an error or changed your mind, click on the {Cancel} button to return to the Setup window ‘with no interruption in Batch operation. | endatch CNBCIABCA2ExaeeT 8087 a = 8.2 Exiting BioCommand Lite If you wish to exit BioCommand Lite without ending all running Batches, an advice window, “Exit with Active Batches”, must be acknowledged before the program will close: Click the {OK} button to exit the program, or click on the {Cancel} button to return to BioCommand Lite. New Brunswick Scientific Users Guide8.3 Restarting with Active Batches Usually a shutdown with Batches running is the result of an accident, such as power failure or operator error. Recovery is easy. 1. Restart BioCommand Lite. On restart, you will see the “Starting BioCommand” opening Wizard screen. (If you have tured it off, skir to pia #2.) = 7 © Click on the option button to select Resume a batch running when BioCommand Lite exited. © Click on {Continue}. The “Resume Batches” window will open (see below) 2. On restart you will see the “Resume Batches” window immediately if you have tumed off the Wizard: Select the Batch(es) you wish to resume. 4, To reinstate computer supervision of the selected Batch(es), you can click on the {Resume Batch} or {Details} button (see step 5 below). If you click on {Resume Batch). the Batch Setup window will open. Expect a brief pause while the program locates and connects to the hardware. The process will continue as it was prior to program exit. ‘You may prefer to click on {Details} to directly enter the Batch Setup window fora selected Batch. This will give you additional options: © Note that this window (see sample below) has two new buttons at the bottom: {Terminate Batch} and {Resume Batch} © This option allows you to modify batch parameters before you resume the batch, or to end the batch. BioCommand Lite M1286-0060 Users Guide84 ‘CSLNOTE: If more than one batch is available to be resumed, the “Resume Batches” window will return. You may select another batch to resume, or click on {Exit}. The “Resume Batches” window can be recalled later by selecting File-Resume Batches from the Main Menu. Shutting Down BioCommand Lite There are two Ways to exit BioCommand Lite: A. Click the “Close” icon (>) in the upper right comer of the Main Screen, or B, Select File ~ Exit from the Main Menu. SLNOTE: To prevent the loss of data, always exit BioCommand Lite before you turn off power to the computer. In addition, always shut down Windows® itself in the prescribed manner. New Brunswick Scientific Users Guide68 THIS PAGE INTENTIONALLY BLANK BioCommand Lite © M1286-0050 Users Guide9.1 New Brunswick Scientific MAIN SCREEN FUNCTIONS This chapter provides an overview of the File, Edit, View, Tools, Window and Help menus available from the Main Screen, The Main Screen also includes an optional foolbar for quick access to several operations. File The file menu, shown below, provides access to the functions that are used to control the recipes and batches that may be used for process control with this program: ea 2 PET EE ET New Batch ~ creates a new set of Batch parameters. New Recipe ~ creates a new set of Recipe parameters. View Recipe — views an existing set of Recipe parameters, Start Options — reopens the “Starting BioCommand” Batch Wizard window. Resume Batches ~ allows you to view the list of Batches still considered open by BioCommand Lite and offers to resume data collection on any of them. Exit — will close the program and exit back to the Windows® operating system. Users Guide70. 9.2 Edit This selection provides the standard Windows® functions that are available in conjunction with the Windows® clipboard. Any item that is selected using normal Windows® techniques can be removed from its current location and saved on the clipboard (Cut), copied from its current location to the clipboard (Copy). cr inserted into the location of the cursor from the clipboard (Paste). A selected item may also be deleted from the screen without going to the clipboard by using the Delete selection. The Copy alll selection will copy everything on the screen onto the clipboard. 9.3 View This selection provides you with several functions related to the display during operation of the program, as shown in the sample window below. BioCommand Lite @ M1286-0060 Users Guide9.4 m1 Leena © Toolbar ~ If there is a checkmark beside the Toolbar label, the Toolbar will ibe displayed on the main window. This is a toggle function: if tis on (displayed), clicking this item will tum it off; likewise, if it is off, clicking here will tum it on, See Section 3.6 or Section 9.7 for further information on the main Toolbar functions. © New Trend View - enter the Trend View creation and editing function, ‘The following are examples of other selections that may appear on this menu. The actual items will depend on the Batch you are running, or the Recipe you are creating or modifying. ‘© Setup.1 — opens the Batch Setup notebook. Alarm Summary. — opens the Alarm Summary sereen for the Batch. «Batch Summary.1 — opens the Batch Summary sereen for the Batch, © Trend.1 — displays 2 defined Trend View screen. If two or more Trend Views are defined, they will all be listed on this menu, with increasing suffix numbers (i.e., Trend.1, Trend.2, Trend.3, ete.), Tools The Tools dropdown menu offers an Offline Data function that may be used in conjunction with the rest of the BioCommand Lite program while running a process. New Brunswick Scientific User's Guide72. 9.4.4 OffLine Data Data collected by techniques or instruments not connected to the operating fermentor may be entered into the database for a batch by using this function. ffLine from the Main 1, While the batch is running, select Tools — Menu. The Offline data window will open: For the time data you enter into the database, there are three time format, settings available. ‘The default time format setting is Calendar, which allows you to input both date and time of the data reading. EFT means that you will enter the time in an HH:MMSS format. Decimal EFT means time recorded as a decimal number. If you wish to change the time format setting, do Step 2. If not, skip to Step 3. 2. Click on the option button of choice for the ‘Time Format’ BioGommand Lite M1286-0060 Users Guide73 Enter the time entry. 4, Move to the Comments column: either press the key, the right arrow key (<->) or the key, or move the cursor (with the mouse) and click inside the desired cell. Valid time entries are from the batch start time through the current time. 5. Enter Comments to describe the data value you are recording. -Fe make your comments more meaningful, you will probably want to add at least one more column for the actual data values. The program will treat this new parameter as another loop; the name you enter here will appear in all loop lists and reports generated by the database for this Batch. To add a column, or “new parameter”: a NOTE: Any New Parameter you add will become a permanent record. There is no function to delete it once it has been entered. 1, Click on the {New Param} button. The “Offline data” window will temporarily disappear as the “New Offline Parameter” window opens Enter the name of the new column (use no more than 6 characters) in the edit box. Click on {OK} (unless you need to {Caneel}). The “New Offline Parameter” window will close, replaced by the “Offline data” window. ‘Your new parameter will appear as a third column, (See sample sereen below.) New Brunswick Scientific Users Guide74. Door Ete New Paar Come : 4. Repeat the above steps to add other columns. Note that you can move the cell borders just as you do in a Word table or an Excel spreadsheet. ‘Now that you have established the records, an offline measured data value can be added to the database at any time during the running of the Batch. To enter a new line of data: 1. Move your cursor to the * in the far left column. 2. Click when your cursor changes to an arrow (>). A new row will appear, and your cursor will be positioned in the time column, 3. Enter the new data, 4, If you wish to delete an entry, you can use the {Clear Edits} button as long as the line is still active. 5. Click on the {Close} button to end the Offline Data function. 9.5 Window Whenever two or more windows are open on the screen within the BioCommand Lite program, this function allows you to view them in a choice of formats. (See sample menu below) BioCommand Lite © M1286-0050, Users Guide75 The available formats are the standard Windows® 98/NT/2000/ME/XP and XP Pro selections: Tile Horizontally © Tile Vertically + Cascade © Arrange Ieons also appears on this menu, This standard Windows® format is not functional in BioCommand Lite The above screen shows the Window menu when there are several windows open but minimized to icons. Any of the windows may be closed, restored to previous size, or maximized to full screen size by clicking on the icon, which opens a submenu with selections for Restore, Maximize, or Close. Double-clicking on the icon will restore that window or view to its previous size. When several windows are open at the same time, selecting Window - Tile Horizontally or Window ~ Tile Vertically will change the screen display. It will be similar to the one shown below. Each window can be individually selected, resized, or restored to full screen size. New Brunswick Scientific Users Guide76 zen Selecting Window ~ Cascade will rearrange the screen display, stacking the windows like a deck of cards. It will resemble the example shown below. Each window can be individually selected, resized, or restored to full screen size. Clicking on any part of a window will bring that window to the top of the cascade. BioGommand Lite @ M1285-0060 Users Guide9.6 9.7 Help This selection provides the standard type of Windows® Help functions. (ee) on ret ent Fe tao Contents — A list of help topics available. Search for Help On — Provides the option of searching for Help using a key word or phrase. © About BioCmd32 - Displays a window of information about the BioCommand Lite program. Toolbar Description The Main Screen of the BioCommand Lite program includes an optional toolbar. This toolbar can be used to initiate several functions within the BioCommand Lite program, through a single mouse click. When you place your cursor over an icon on the toolbar, a hint appears to remind you of that icon’s function. ‘The toolbar can be toggled on and off: 1. Select View — Toolbar from the Main Menu. If the toolbar is on, there is a checkmark beside Toolbar on the submenu. Click on the checkmark to remove the toolbar from the screen. The checkmark will also disappear. 3. To redisplay the toolbar, select View - Toolbar from the Main Menu. Click in the checkbox to the left of Toolbar. 5, Accheck will appear in the checkbox, and the toolbar will appear onscreen. v New Brunswick Scientific Users GuideBelow is a picture of the toolbar and its various functional icons: New Batch New Recipe a ee Hi What's This? HELP eet va Bsisix| s(mlelx| ala| 2.” Cut Copy ‘Standard Windows® functions. Print 2S. NOTE: Each of these icons performs the same function as the similarly named selection from one of the menus. BioCommand Lite © M1286-0050 Users Guide79 10 RECIPE/BATCH FUNCTIONS BioCommand Lite allows you to enter a variety of run parameters and ____ save them for future use without having to re-enter the parameters each time a similar set-up is desired There are two componenis‘to this function: Recipes and Batches. A Recipe is a set of parameters that define the fermentation process. A Batch is a single run based on that Recipe. The same Recipe may be used for any number of Batches; and each Batch must start with a Recipe. 10.1 Creating or Editing Recipes ‘You can create new recipes or edit existing recipes by using the Batch Wizard. ‘The Wizard is active as soon as you open BioCommand Lite, unless you have cancelled it. If it does not appear and you wish to bring it back, select File ~ Start Options on the Main Menu, or you can click on the toolbar Batch Wizard icon. Refer to Chapter 4 for instructions on using the Batch Wizard. ‘You can also create new recipes using the New Recipe function or you can edit existing recipes with the View Recipe function, 10.1.4 New Recipe Setup Window Click the New Recipe icon on the Toolbar or select File ~ New Recipe from the Main Menu. The “Setup” window will open: New Brunswick Scientific Users Guide80. This is a dialogue box with 4 tabbed pages. Each page is a new window with different functions. These pages are: Recipe ~ for the entry of basic recipe parameters Loops — displays the loops that are available and allows you to select the loops to be included in this recipe. Views ~ allows the creation and editing of Trend views. Also provides an entry to display these views, as well as the standard views that are included in the program: Alarm Summary, Batch Summary, and Setup. © Alarms ~ allows the setting of Alarm levels for any of the loops included in the batch, This section provides detailed instructions on using the various tabs. 10.1.2 View Recipe Setup Window 1. Click the View Recipe icon on the Toolbar or select File - View Recipe from the Main Menu. The “Select File to Open” windew will open first, listing the existing Recipe files: Eee allies Site Tal Reciness ‘Siew Brepesie Brecowtico — [slReopesa lrcoedieo Resto Z]Respeics — Aeceee Blecoesep a Reorete a Be] Fleectype FRCP Fit ACR I coroe! I 2. Select the Recipe of choice: click on its name or type the file rame into the File name edit box. 3. Click on {Open}. The “View Recipe” Setup window will open. This window is the same as the “New Recipe” Setup window shown on the previous page. Note, however, that the fields are filled in with the parameters of the Recipe you selected. BioCommand Lite @ M1286-0050 Users Guideet 10.1.3 10.1.4 Using the Setup Window From this point forward, the functions are essentially the same, whether you are creating a new Recipe or editing an existing one. ‘The four standard buttons at the bottom of the window are visible at all times, regardless of which notebook page is displayed. These four buttons “ate: ¢{Save As} — this function will save the Recipe under a different name than the original Recipe. @ {Save} — this function makes your changes to the Recipe parameters effective for the Batch. © {Start Batch} — this function will start a Batch run, using this Recipe’s parameters. This button will not be available unless the Recipe contains enough information to run a Batch. When a Batch is running, this button changes to {End Batch}. {Close} — This will close the Setup screen. If you click on {Start Batch} or {Close} but you have not saved the Recipe changes you have made, the “Save Changes” window will open: Seve Changes To aobe? © Ifyou click the {No} button, all of your changes to the Recipe will be lost. © Ifyou click on the {Cancel} button, the program returns to the Create Recipe or View Recipe function, whichever you were using. © If this is a new Recipe and you click on the {Yes} button, the “BioCommand Save Files” window will open. Enter the file name under which you wish to save the new Recipe Recipe Tab The Recipe page of the notebook (see sample below) contains items that are considered descriptive of the Recipe: New Brunswick Scientific User's Guide82 ‘File Data’ Pane: The edit box in the ‘File Data’ pane displays the name under which the Recipe will be saved. If this window was opened via the View Recipe selection, this box will contain the name of the Recipe file that was opened. If, instead, you have created a new Recipe, this box will be blank until you save the recipe file to disk and you fill in the “BioCommand Save Files” window. * Ifyou are going to use an existing Recipe as the starting point for a new Recipe, click on the {Browse Recipes} button to bring up the “Select Files to Open” window. This window allows you to choose the Recipe file you wish to use. © You may also use normal Windows® techniques to navigate through the disk folders, to locate the desired Recipe file. File Data pane Recipe Data pane rasa Recipe Data | oiparenNane tab Time Data le tab I Timadas | Recoe Dae bola hia) ~Canet FT ———— Tein Foret © ems owe FT © Decenlieus | Charged BioCommand Lite © M1286-0050 Users Guide‘Recipe Data’ Pane: The ‘Recipe Data’ pane contains two items: © The ‘Display (Tab) Name:’ is a descriptive name that you give to the Recipe. This is the default name that will appear on the tab at the bottom of the screen when a Batch using the Recipe is active. It is also ~~~ the default name suggested for use as the file name for data storage This item may contain up to twelve alphanumeric characters. © The ‘Description:” item gives you the option of entering text that more completely describes the function or special features of this Recipe. It may contain spaces and punctuation marks as well as alphanumeric characters. Near the bottom of the window is a pane that contains a sub-notebook with two tabs, ‘Time Data’ and ‘Recipe Data’, as shown in the sample screen above, ime Data’ Tab: The “Time Data” tab allows you to select the time interval between data point acquisitions. 1, Click on the {Change Interval} button under the edit box. In the Change Log Interval screen that appears (see below), you have two choices: A. Use the edit box down arrow (¥) to open a list of time selections and choose one by clicking on it. B. Enter any whole number from 1 to 99 directly into the edit box Jabeled “Log Interval (Mins)’. New Brunswick Scientific Users GuideWhen a Batch is running, this pane also continually shows the Elapsed Fermentation Time (EFT) in the ‘Current EFT” box. ‘The {Reset EFT} button, which will reset the EFT to zero, is available to you only when a Batch is running: 1. Click on it at any time during the Batch run. The “Reset Eft” window = will open: . Feaethe Eerie back? aaa 2. Click the {OK} button if you want to turn the clock back to zero, or click the {Cancel} button to abort this action. This tab also allows you to select the format for EFT display. On the far right, in the pane labeled “Time Display Format’, there are two selections: A. HH:MMSS displays the EFT in the format of hours, minutes, and seconds (ex: 12:59:03). B. Decimal hours displays the EFT as a decimal fraction (ex: 12.98), If itis not already selected, click on the option button next to your format choice, ‘Recipe Data’ Tal After a new Recipe is created and saved, the ‘Recipe Data’ page of the sub-notebook (see below) will display information entered while the Recipe was being created: BioGommand Lite M1286-0060 Users Guide| seen] 2 se Seiten | cow © The ‘Recipe File:” box displays the file name under which the Recipe is saved. The ‘Date:’ box displays the time and date when the Recipe was created, Nothing on this page can be edited; itis an information display only. 10.1.5 Loops Tab ‘The Loops tab opens up the notebook page where the loops to be used in the Recipe can be selected or modified. There are two panes on this page: “Change Loop List” and “Current Loops’ (see sample screen below): New Brunswick Scientific Users GuideWhen you open this page, if no loops were previously specified for this Recipe, the “Add/Change Hardware Loop List” window will open over it ae ‘This window is used to tell BioCommand Lite where to look for loops that may be used with this Recipe. 1. Click on the {Browse Local Server} button to display loops that are available to your PC. ‘The sereen shown below is an example of a system connected to two local controllers. Loops that are accessible through this connection are displayed in the left pane, which is called ‘Available Loops:". BioGommand Lite # M1286-0050, Users Guide| change Caynt Hardnere Loop it EMERSON a Comelean, = Conwoleas 1, Click on the + box next to the controller name (or double-click on the controller name). An expanded list will drop down, showing all loops associated with this controller and therefore available for your Batch: S\NOTE: Be sure to expand all controller loop lists to see everything available to you before you complete your selection. 1. To select all the loops associated with a controller, click on the controller name, then click the {>>} button. 2. To select individual loops from one or more controller(s), click on the desired loop name, then click on the {>>} button. a New Brunswick Scientific User's Guide3. Each time you select an item and click on {>>}. that item will transfer to the right pane, “Loops in Batch:’. When you expand the controller loop list, the + sign becomes a ign. To collapse the expanded list, click on the - sign. The list wil fold back into the controller name, leaving a + sign again., eae Avaisble Lops To remove a loop from the ‘Loops in Batch’ pane: * Click on the loop name in the right window pane * Click the {<<} button. The loop will move back to the left side, which removes it from this recipe. 4. Afier all of the desired loops are in the Recipe, click on the {Close} button to return to the Loops page of the ‘Setup’ View The loops you have selected will now be listed in the ‘Current Loops” pane (see sample below), in the following way: BioCommand Lite @ M1286-0050 User's Guide| ‘© The loop name (default to the controller) appears on the left side of the window pane. © To the right of the loop name is the location of the loop. The location is listed in the format of “(Server Name\Conttroller ‘Name\Loop Name)”. ‘You now have the option of assigning new Display Names and initial Setpoints to the loops for this Recipe. If you do not wish to change the default controller loop names and setpoints, skip the following steps (5-9): 5. Click on the {View loop details} button or double-click on any loop name. The “Loop Setup Options” window will open. When there is no active bactch, this window allows you to set a Display Name and an Initial Setpoint for each loop, The window is inactive while a batch is running. Eo] | Loon: [agit (EWERSONS\Conmote23Vigt 1) a Dit =| | I New Brunswick Scientific Users Guide90. 2S.note: After each loop is edited, it is necessary to click the {Apply} button to make the changes effective. 6: To set a loop Display Name that is more meaningful to you than its controller default name: 2 © Cick in the ‘Display Name:’ edit box. © Change the name there to any combination of letters, numbers and spaces, up to a length of 10 characters. 7. To set an initial setpoint for a loop that is different than its controller default setpint: Click in the ‘Initial Setpoint:’ edit box. Enter the desired setpoint. 8. Continue editing these parameters for other loops without leaviag this window: click on the down arrow (¥) on the right side of the “Loop:” edit box, then select the loop name from the dropdown menu. 9. When you have finished editing, click on {Apply}, then click on {OK}. 10.1.6 Duplicating a Recipe The Loops Tab can also be used to easily duplicate a recipe for use on another fermentor that is connected to the system: 1. Click on the {Replace loops in this recipe with those from another controller} icon on the Loops page: BioGommand Lite @ M7265-0050 Users Guide34 ar EaRSaaeT Fa [Eveasouncoroman i Bod + [EvensouacireeaRS0? 9) | Fes [Euensovncrras Af an! [Euensmuncorenan on’) Vat [Euensovacrnts sen [Euensonscoeetezeos 1) | tot [Euahsonscrele zee 2, Click on the down arrow (¥) at the right side of the “Replace:” edit box. 3. From the dropdown menu, select the controller you want to replace. 4. ‘Now select the new controller from the controller names in the ‘With:” 5, Click on the {Apply} button to effect the change. 6. If there are any loops in the original controller that do not have equivalents in the new controller, the “Error Replacing Loops” window will open to inform you of the discrepancy: New Brunswick Scientific Users Guide92 were Leone recipe not found innomist: ‘Bibesoks oem! MERSONE\Coniole22\Levst- EWERSOND\Cortole 2B Stor Delete these loops om ecne end Contre? Ce» | ‘© Click the {No} button to abort the replacement operation, or © Click the {Yes} button to delete the loop(s) from the loop list, in which case the following confirmation message will appear. Click the {OK} button. ‘After the loop selection process is complete, the Loops page on the Setup view will resemble the sample shown below: eae ‘The “Current Loops” pane now lists all of the currently selected loops: BroCommand Lite © 1286-0050 Users Guide93 ‘* On the far left side is the display name of the loop. This will be either the default name of the loop from the controller, or the name as edited on the “Loop Setup Options” screen. * The label to the right of the pane is the location and assigned name for each loop. The format for this label is “(Server Name\Controller NameiLoop Name)”. 10.1.7 Views Tab ‘The Views Tab opens the notebook page that allows you to create several types of views or to open default views established by the program. This page (see sample screen below) consists of two panes: ‘Current Views:” and ‘New Views:". “Current Views’ Pane: ‘This pane lists all of the views that are available by default and those that have been created for this Recipe. Initially there are three Views which are standard with each Recipe: Alarm Summary, Batch Summary, and Setup: The Alarm Summary View is a table summarizing any alarms that are active while a Batch is running, This view is available while the Batch is running, © The Batch Summary is a table summarizing the loops used in the Batch. It is available while the Batch is running. This displays the Loop Name, Current Value, Setpoint, Output Value and Control Mode. The Setup View is the notebook you are already using: it contains the {our tabs that open the pages vou use to enter parameters for the Recipe and to control the Batch. Below is a sample Setup View, opened to the Views tab. New Brunswick Scientific, User's GuideSapo ae ‘New Views’ Pane: You can use this pane to add a new Trend view to a Recipe or a Batch: 1, Double-click on Trend to open the screen, You can then create a new view. 2. Chapter 6 gives detailed instructions on how to create and modify Trend Views. 10.1.8 Alarms Tab BioCommand Lite allows you to enter high and low Alarm limits for any of all of the loops in your recipes, while a Batch is running. Alarms inform you when a loop value enters a dangerous range. When a loops value exceeds your preselected Hi Limit or drops below your preselected Lo Limit, the Alarm display tab (see sample screen below) turns red to signal a visual alarm. If you have tumed the audible alarm ‘on, you will also hear an alarm signal. There are three ways to see what triggered the alarm: A, Select View - Alarm Summary from the Main Menu; BioCommand Lite M1286-0050 Users Guide95 B. Click on the Alarms tab in the Setup view; C. Click on the Views tab in the Setup view, then click on Alarm Summary in the ‘Current Views’ pane; The “Alarm Summary” window will open to the Current Alarm Settings page. ‘See Chapter 7 for detailed instructions on setting Alarms. 10.2 ‘Starting a Batch ‘2S. NOTE: There are four ways to start a Batch; whichever method you choose, each Batch must always begin with a defined Recipe. The options for starting a Batch are: Batch Wizard, New Recipe, View Recipe and New Batch. 10.2.1 Batch Wizard See Section 5.1 to start a batch using Batch Wizard. New Brunswick Scientific Users Guide10.2.2 New Recipe or View Recipe You may create a new recipe, modify an existing recipe, or use an existing recipe following the procedures in Chapter 5 When the Recipe has the minimum amount of information needed to completely define a process, the {Start Batch} button on the bottom of the ~ Window will become available” After you click on {Start Batch}, the program will ask you for some additional input (see Section 5.4). After you comply, the “Recipe” tab on the Setup view will change to “Batch”, the {Reset EFT} button will become available, and the {Start Batch} button will become the {End Batch} button, 10.2.3 New Batch S.noTe: Following is a brief recap of New Batch procedures. Be sure to consult Section 5.4 in-detail before starting a new batch. Aside from using Batch Wizard, you can start a New Batch in two other ways: A. Select File — New Batch from the Main Menu; B. Click on the New Batch icon on the Toolbar, The “New Batch Setup” view window will open, with the “Select File to Open” window on top of it. The top window lists the Recipe files that are currently available for use. 1, Enter the desired Recipe file name in the ‘File name:” edit box. 2. Click the {Open} button. The selected Recipe file will be loaded into the program and the {Start Batch} button will become available. You may start the Batch immediately, or you may change Recipe parameters and then start the Batch. After you click on {Start Batch}, the program will ask you for som= additional input (see Section 5.4.3). After you comply, the “Recipe” tab on the Setup view will change to “Batch”, the (Reset EFT} button will become available, and the {Start Batch} button will become the {End Batch} button. BioCommand Lite @ 1286-0050 Users GuideOnce the Batch begins to run, operation is the same regardless of which method was used to start it. The “Current EFT” box on the Batch page will start displaying the elapsed time of the process and the View selection from on the Main Menu will list all of the views that are available at the time. 10.3. Altering a Batch in Progress ‘When a Batch is started, its parameters are those preset in the Recipe selected, plus any modifications that may have been made to the Recipe prior to Batch startup. Most of these parameters may be changed while the Batch is running, essentially by using the same procedures as those originally used to set them in the Recipe. 10.3.1 Changing Setpoints One of the changes most often made during the running of a Batch is the Setpoint for one or more loops. 1 Open the Batch Summary View, using one of the following means: ‘A. Click on the display name tab at the bottom of the Setup window for the Batch being run; B. Select View ~ Batch Summary from the Main Menu; C. Click on the Views tab in the Setup window, then select “Batch Summary” from the ‘Current Views:" pane. When the Batch Summary window is open, double-click anywhere in the row of the desired loop (Agit 1 in the sample shown below). The “Change Setpoint / Control Mode” window will open: New Brunswick Scientific Users Guide- be ees Agit 1 e Control Mode: PAD. Supe_by BioCommand © Locally 2t Fermentor Setpoine fis a S\NOTE: BioCommand Lite cannot change the loop setpoint unless the option Supv. By BioCommand Lite (“Supervised by BioCommand Lite”) is chosen in the ‘Controlled’ pane of this window. 10.3.2 3. Click on the Supy. By BioCommand Lite option button if it is not already selected. Now enter the new setpoint value in the Setpoint edit box. Click on the {OK} button (or press the key on your keyboard). > BioCommand Lite will send the new setpoint to the controller; the Batch Summary window will display the new setpoint value; and you should also see the Current Value responding to the change in setpoint value. Editing Trend Views To edit a Trend View, you must first make that view active by one of the following methods: A. Select it from the Views option on the Main Menu, B. Click on the Views tab in the “Setup” notebook window. When the view is displayed, edit it using the techniques described in Section 6.2.7, Modifying a Trend View. ‘The changes you make to the view become effective immediately, but only as a part of this Batch; they are not added to the original Recipe that was used to start the Batch, BioCommand Lite @ M71286-0050 Users Guide99 10.3.3 Adding Views To add Trend Views, begin the process by one of the following two methods: ‘A. Select the Views tab from the “Setup” notebook, then click on Trend in the “New Views:” pane. B. Select New Trend View from the View submenu on the Main Menu. ‘A new Trend graph window will open. You can design it as explained in Section 6.2.1, Trend Views. ‘The added view(s) will only be a part of the current Batch; the original Recipe used to start the Batch will not be modified. 10.3.4 Deleting Views To delete a Trend View from a Batch: 1. Open the desired Trend View from either the Views tab on the “Setup” view or from the View selection on the Main Menu. Place your cursor anywhere inside the View. Right click to open a submenu. Click on “Delete View” in the submenu. You will be prompted: “Permanently delete this view?” Click on {Yes} to complete the deletion or {No} if you have changed your mind, This will clear the View, removing it from the “Current Views:” pane on the “Views” page of the Setup notebook and from the list under the selection on the Main Menu. After this is done, the deleted view will no longer be available to the Batch being run. The View will only be deleted from this Batch, however; it will remain part of the original Recipe used to start the Batch. New Brunswick Scientific Users Guide100 10.3.5 Ending a Batch There is only one way to end a Batch: 1. Open the Setup window. 2. Click on the {End Batch} button. “When you do this, the program will automatically request confirmation before completing the command: : Ei End Batch CABC32ABC32E sample! BDB? 3. Click on the {OK} button to confirm your desire to terminate the Batch, or click on the {Cancel} button to save the active Batch from termination. (The Batch will continue to run until you choose.) If you cancel, the program will return you to the “Setup” window with no interruption in Batch operation. ‘When you end the Batch, the data logging files and all view windows will close. Snore: If you exit BioCommand Lite without ending a Batch that is running, the following window opens before the program shuts down: Emirs There are active batches currently datalogging onthis workstation. Exiting wik end data log. Ext anyway? zeal Clicking the {OK} button will exit the program and clicking the {Cancel} button will return to the existing operation. While data logging will cease if the program is exited, the program remembers the Batch parameters, so when BioCommand Lite is started the next time, you will be offered the opportunity to resume the batch. BioCommand Lite M1266-0060 Users Guide101 Resuming a Batch If you have not disabled Batch Wizard, you can use the “Starting BioCommand” screen (shown below) to resume a batch that was running when you exited: “> | ‘Want to rama fermentation batch? First youneed.arecipe. -You.can: © Croan rr ace nth Batch Wired © Opanatlenk ecb © Opmnan ising ecpe © Eict arespetem a batch If you have disabled Batch Wizard, the following window will appear as soon as you start up: Resume Sach Date To resume immediately 1. Click om the {Resume Batch} button. The Batch Setup View window will open, and there will be a brief period while the program locates and connects to the hardware BioCommand Lite will resume control of the running batch and resume data logging (from where it was when the program was shut down). The process will then continue running as it has been prior to the program exit. If you prefer to review information about the batch prior to making a decision: New Brunswick Scientific Users Guide102 1. Click on the {Details} button. The “Setup” jew window will open: + = Tinie Fea 6 Hames, — This screen gives you the option of resuming the batch, modifying the batch parameters before resuming, or terminating the batch Snore: When you resume a batch which has remained active while BioCommand Lite was closed, the total EFT for the hardware since the batch was started will still be accurate. The data log file, however, will have a gap for the period of time that BioCommand Lite was not controlling the process. 10.3.7 Changing Data Logging Criteria The only Data Logging criterion that you may change is the Log Interval. You may change the interval at any time, even while the batch is running: 1. Open to the Recipe tab or the Batch tab in the “Setup” view. 2. Click on the down arrow (¥) in the ‘Log Interval (Mins)’ edit box, in the ‘Time Data” pane, and choose a time interval from the dropdown list; or enter a whole number from 1 to 99 directly into the edit box BioCommand Lite @ M1286-0050 Users Guide103 11 TROUBLESHOOTING Ifyou find that BioCommand Lite does not work, or does not respond ‘properly, there are a few possible reasons you can easily check and resolve yourself. If the following recommendations do hot eliminate your difficulty, please contact your NBS Representative or NBS Service. 11.1 Troubleshooting Guide If you encounter any of the following symptoms during initial set-up, or at any other time while you are working with BioCommand Lite, try the suggested solution(s) before you call for service: ‘Symptom Possible Solution ‘No feedback is available from fermentor. ‘Make sure power is connected. Check all settings as specified in the system Set-Up instructions. Check version of Bclocal (minimum of 2.X is required), Check settings on local controller. Workstation performance is slow. ‘Communication between the workstation and the fermentor has | been lost. | ‘There may be too many fermentors on one workstation. Try reducing to fewer than six. Check power to the fermentor. Check whether the server has disconnected the workstation. Check cabling, Fermentor loops are not reporting. ‘Make sure that the loops are not engaged in a batch that is still running. | Only 10 loops are reporting. New Brunswick Scientific Ifyou are using an AFS Interface box, this is normal. The box can only handle 10 | Users Guide104 Symptom Possible Solution Ceriain files do not self-register. | e These files may be running in the background. Run Task Manager to verify that the files have stopped, then reinstall BioCommand Lite. ¢ In Windows” NT 4.0 or 2000, make sure that the logged-on user has administrative privileges on the computer, then reinstall BioCommand Lite. . WINDOWS® 98 ONLY: Make sure DCOMCNFG is installed. Unable to connect to the fermentor. If you have encountered a problem not covered in this guide, or if the solutions proposed above do not resolve your difficulty, please call your NBS Representative or NBS Service. BioCommand Lite © M1286-0050, Users Guide105 12 SPECIFICATIONS & SYSTEM REQUIREMENTS 12.1 BioCommand Lite Specifications Product type Bioprocess supervisory software Code 32-bit [Media CD-ROM [Compatible Operating Systems ‘Windows® 98, NT4.0, 2000, ME, XP & XP Pro | Supported Products ‘All New Brunswick Scientific computer oe capable products 12.2 System Requirements 12.2.1 New Brunswick Minimum Computer Requirements © PC, 350 MHz Pentium II © 64MBRAM © 100 MHz bus * CDROM © 1GB Hard Drive © 1L4MB 3.5" drive ‘© Mouse or equivalent pointing device © Sound card, Operating System-compatible © 38KBRS232 COM port © 15" monitor © 800 x 600 SVGA, 256 colors For direct communication with 16-loop ML-6100-type controllers add: © 10MBLAN adapter, twisted pair TCP/IP protocol. Scientific Users Guide108 1222 Recommended Computer As indicated in Minimum Computer Requirements above, except that we recommend the following for best performance and user comfort: © PC 500 MHz Pentium IIT © 128MBRAM © 4GBHD © Larger monitor and higher resolution to suit display requirements 12.2.3 Computer Operating Systems © Windows® XP Windows® XP Pro © Windows® 2000 «Windows NT® 4.0 * Windows® ME or * Windows® 98 BioCommand Lite © M1286-0050 User's Guide107 13 DATA LOG FORMAT Batch data is stored in a format compatible with the Microsoft Access® database management program. The figure below shows the list of tables generated for @ typical Batch. Any program that can read Access format data may use the data from a BioCommand Lite process. The data may be manipulated and displayed in any of the formats allowed by the database program. BioCommand Lite uses the same database as BioCommand 32, but does not have all of the same features, particularly those related to network connectivity and authorization levels. For this reason, certain tables will contain no data. eae [Elle Galt view Insert Tenis Window: Help er [Oe O/SA7)} Ses) B-/a-|> sms) See rabies | BP Queried EE Forms | abereetincs AbrtSetings sachevents aacho cascadesetnge Changerocord currents errors Log sot 1 Log.Ar 1 eg.002 3 tog_Foamn 1 Log_Nuté 1 loge Log Pres 1 LogTimes Loops Marchanges offiredatz Opercharges ReportGraphs ReportSattings swrileesetings ‘SupyChanges ‘SupyDiagrams Useraccass UseripDiagrams aSa9g08095088q a a a a a a a a 5 a a a a a New Brunswick Scientific User's Guide108 In addition to the Batch loop data, the database contains all of the parameters that were used when the Batch was run, no matter what the starting Recipe parameters were. The contents of the various tables are described in the following sections, 13.1 Settings 13.4.1 AlarmSettings i If you have set alarm parameters for any loops, this table will list them. ‘The format of this table is very similar to the format of the table you used to set the alarms in BioCommand Lite. Loop | Hilim | Lokim.[ Type | Enabled |LoShutdownList ¥iagt + ‘800 500 Absolte | 1 lair 1 40. 10 Absolute 0 Temp 4 40 25 Setpt Dev 0 ° Record: KL A|[ 1 Leder 3 13.4.2 CascadeSettings Not used in BioCommand Lite. 13.1.3 SterilizeSettings Not used in BioCommand Lite. 13.2. Changes 13.24 ChangeRecord This table (see sample below) lists any changes that have been made during the process of running the Batch. The ID column consists of consecutive numbers for each record in the file logged by BioCommand Lite as each action is recorded, The next column lists the calendar date and time of each action. The Operator column will always say “Admin”. The next column lists the action that was performed, and the following columns contain data related to the action. BioCommand Lite © M1286-0050, User's GuideFE] 1 Wae 12 eas Pw eCs2Mcnve Change user prieges aan Manage 2 awe 120848 2M aan Charge dala teal [7] vag 120850 >Meann _Chageviow Sept 8CS2 Exarpe Seup [1 4 99s 20059 emeann Chango ven Bach Summary 8C52 Exar ech Sa TD Sees 120850 wean Set Datu vow Bach Summary Fs vag 208sepMeann change vew ‘Norm Surety C52 Example Alam Sa I]t tao0 20042 pw edn Grange uv ara ‘at ‘oie sem are ¥ [sue ‘Normal ° [Testes Summary Nera! 150 [parm Surmanyhicrmat 1300 Jagtaton Seto el sei —"—r leat 7 TRUE| O)OuBK) + voit | JT sl 13.2.2 MgrChanges Not used in BioCommand Lite 13.2.3 OperChanges Not used in BioCommand Lite. 13.2.4 SupvChanges Not used in BioCommand Lite. New Brunswick Scientific Users Guide110 13.3 Batch Events 13.3.4 BatchEvents This table lists the time and description of any and all Alarm events that occur during the running of a Batch. (The “Alert” feature is not used in BioCommand Lite.) Time | EventType “S190 12:20:03 PM Lew Alarm —— Aait 1 ‘(96 12.28.05 PM Normal Alarm Agit 1 112/99 12:29:55 PM Low Alert “Ag 1 43/99 12:32:23 PM Normal Alert Agit 1 1/3/99 12:39:19 PM Low Alert, Aait 1 113/98 12:44:37 PM Normal Alert_Agt 1 Reoe KIA] 1 DIM pSJore 13.4 Reports 13.4.4 ReportGraphs Not used in BioCommand Lite. 13.4.2 ReportSettings Not used in BioCommand Lite. 13.5 Data Logs 13.5.1 Loop Data The loop data are stored in tables that are individual to each loop. Each table consists of the log ID number (LG_ID), and the actual data value (Value). Each loop table will be named “Log_[loop name]". The loop name is the unique display name you have assigned to the loop. In the sample screen shown below, the name is Log.Agitl. ‘The log ID number is the number of the data log and is incremented by BioCommand Lite with each acquisition. The time of each log item can be determined from the LogTimes table described in Section 13.5.2 below. BioCommand Lite © M1286-0060 Users Guidecc Value 4 667.5 2 973.07 3 m9) 4 625.39) 5 602.99) 6 $51.29} if 8 9 0 4 466.89) 452.03} 450.21 41 450.02) i AAs 161 oo Record: lal 2 bl oubsforas 4 13.5.2 LogTimes This table contains the information that identifies the calendar date and time that each data log was performed. This data can be used to correlate the loop log data through the LG_ID parameter. LG_ID LogTime * 1/3199 12:09:57 PM 1/3/99 12:10:58 PM) 143/99 12:11:58 PM| 113199 12:12:57 PM 143/99 12:13:57 PM| 1/3/99 12:14.58 PM 1/3/99 12:15:58 PM 113/99 12:16:58 PM 413/99 12:17:58 PM 1/3/99 12:18:57 PM 11 1/3/99 12:19:58 PM ba Wall 72 bts} Seen smmsens 1 Record: New Brunswick Scientific Users Guide12 13.5.3 OfflineData Any Offline data that you enter while the Batch is running is logged into this table. The table very closely resembles the window in BioCommand Lite that you used to enter the data. Any additional parameters that you added to the Batch via the Tools function will also be shown as another column in this table. Calendar_Ti EFT_HMS._ | EFT_DecHrs > Record: Hf 4 1 > [onpps| of 2 13.6 Batch Identifications The Batch identification tables contain the basic setup information pertaining to this Batch, 13.6.1 BatchiD The BatchID table is shown below. This table contains the important data that describes the overall use of the Batch, ee [_Endrime _/BatchOwner FT "7s789 12.4532 PM aamin CABCS2E ample RCP acmn NRL RE ET BioCommand Lite @ 1286-0050 Users Guide113 13.6.2 Loops ‘The Loops table lists the loops that you selected for use in this Batch along with pertinent information about each. This is the information that you entered into the Batch from the New Batch function prior to starting the Batch run. [Units CorcoleA SPTESTS__RPw_ Conroier28a Um CConcber26a 00 CConroter268 Conrolar24a mum Conoec2sa oH Conroe Contoec24 STS age 13.6.3 CurrentStatus This table holds the most recently acquired data for each loop while a Batch is running. At the end of a Batch, it contains the last set of data acquired. All of the data in this table is available in other tables in the final database, but this table allows remote workstations to access the data while a Batch is running. BAM ByBiecommend Nema! Nora By BCormand Nemei—_Nora 41.997 800 8) BlaConmenc Nome! Norma 0 AlFernerter Normal ‘Normal 25min ‘auFermerter Normal Normal Sep fatFernenter Normal Normal 1080 ‘atFermenter Normal. Normal 37 Deg 3 eP.0 AiFemnertsr Normal Normal races tele eben New Brunswick Scientific Users Guide114 13.7 Errors ‘The Errors table is the repository for any errors that are reported by the Batch Manager Server. [TEE STE 13.8 Views Data This table is a list of the views that were defined to be a part of the Batch. It contains the elements that make up the view. (Program and Synoptic views are not used in BioCommand Lite.) Name. ‘Type |. Description [Alar Surmaty 1 BC32 Bamps Alem Summary eich Summary 1 BC22 Exarels Batsh Summary Program? GC32Exarple Program SPAGE II) Iseun ? BCS? Example Setup ISvncote.! C32 Exapie Syremte ——_Gaugolvessal Evernie Treed Pester|213)6 tears Dafoe + Normal 0 Norma: Norma: Norma ° BioCommand Lite @ M1286-0050 User's Guide115 14 VIEWING BATCH DATA The Database Viewer utility, installed when you loaded BioCommand Lite, is a tool that allows you to view data from any ~~ batch—currently active or previously run. You can use this tool to organize and present data in a variety of ways. “It is a quick and efficient way to generate reports, to use the Access®-compatible database or to move data into an Excel® document. ‘As noted in the previous chapter, BioCommand Lite uses the same database as BioCommand 32, but does not have all of the same features, particularly those related to network connectivity and authorization levels. For this reason, certain tables will contain no data 14.1 Starting DBViewer32 Select Start }Programs > Nbs > DBViewer 32 There will be a brief DBViewer start-up screen, then either the “Existing Batches” window (see Section 14.2) or the “Main Data” window (see Section 14.3) will open. 14.2 Existing Batches In the “Existing Batches” window, select the desired batch from the list in the pane, then click on the {OK} button. [se ovaie New Brunswick Scientific Users Guide116 (if you do not see the batch you want, see Section 14.4 below.) DBViewer’s data window will open. 14.3 Using the Main Data Window Central Data Pane Dropdown ‘Menus “a 7.2 ‘of 20.78 a 14 007325) of 2-8) i If Batch Selection | [3] Box | | | @) Breese sieise siersto Settings B utton Tae scroll through data Export to Excel Button Following are descriptions of the various features of this window. 14.3.1 Browse Button At any time, you can click on the {Browse} button to access the “Browse for More Batches” window: BioCommand Lite @ M1286-0050 Users Guide17 14.3.2 14.3.3 ‘This functions the same way as the “Browse for Batches” window you have already encountered. To use the “More...” selection for batches not listed in the window, see Section 14.4 below. Batch Selection Box The edit box over the {Browse} button is another way to access available batch records. Click on the down arrow to open a dropdown menu of available batch records, then select the batch of interest. All data in the ‘main data window will update to reflect your new selection. This is a quick way to toggle between batches, Settings Button If you click on the {Settings} button, the “DBViewer Settings” window will open: New Brunswick Scientific Users Guide118 Time = NST Heaie= Time Stent; [00:00:25 Feora Pncard: 7 Tima Eng:[003127 | Te Recare: 2 The Time Log indicates the period of time during which the available data has been logged. ‘You can use the Settings pane to select a particular range of records for review. Naturally. The “To Record:” entry may not exceed the indicated “Max. Records” number. You can also change the way the logged time is displayed by clicking the option button for Decimal Hours or Calendar Time instead of the default hours/minutes/ seconds (hh:mm:ss). The control loops under BioCommand Lite’s supervision in the selected batch can be removed or returned to the Central Data Pane for purposes of data management. To select or de-select loops. click on the {Select Loops} button. The Selected Loops screen opens: BioCommand Lite # M1286-0050 Users Guide19 AVAILABLE Loop Records 14.3.4 SELECTED D0 4” Loop Records To select a loop that is not displayed in the Central Data Pane: single click on the name and, holding the left mouse button down, “carry” the record over to the Selected list; “drop” it there. You can also double click on the loop name in the Available loop list, and it will appear in the Selected loop list. ‘To remove a loop from the Selected list: single click on the loop name in the Selected list and, holding the left mouse button down, “carry” the record over to the Available pane; “drop” it there. You can also double click on the loop name in the Selected loop list, and it will disappear from ‘that pane. ‘After you make any changes in this window, click on {OK} to apply them and to return to the Main Data Window Export to Excel Button Clicking on the {Export to Excel} button will automatically set up a Microsoft Excel” document that contains all of the current data records displayed in the central pane of the main data window. You will then be able to manipulate the data records with all available Excel features, including the creation of graphs. If you do not want all of the data records in your Excel document, use the ‘mouse to select the records of choice before you click on the {Export to Excel} button, New Brunswick Scientific, Users Guide120 14.3.5 14.3.6 If you do not have Microsoft Excel®, you may use the File -— Copy command from the DBViewer Main Menu to place data records on the Clipboard. Then open a document in the desired program and paste the records into your document. Similarly, you may use the File ~ Save As command from the DBViewer ‘Main Menu to save highlighted data as the file of your choice. Central Data Pane This pane displays the data logged from the moment the batch was started until, (a) for a currently active batch, the moment you opened the DB Viewer or, (b) for a previously run batch, the moment the batch was ended. The data is provided in an Excel®-compatible format. You can use yout mouse to resize the columns according to your needs, and use the bottom and right side slider bars to scroll through the records. In addition to removing and restoring data columns as explained above, you can change the order of the columns: 1. Right click on the loop name at the top of the columa. The cursor will change into a hand holding a sheet of paper. Holding the right mouse button down, move your cursor left or right to the desired location, then release the button. The entire column of data will now appear where you have placed it, ‘You may not move the first or second column (data log numbers and Log Time records). DBViewer Main Menu ‘There are three dropdown menus available from the Database Viewer Main Menu: File, Options and Tools. BioCommand Lite © 71286-0050 Users Guide121 ‘A. Use the File dropdown menu: to access the following commands: jrowse for More Batches” © Qpen: This command will open a window. Print: Use this command to print whatever data records you have highlighted with the mouse. © Export to Excel: This command has the same function as the (Export to Excel} button (see Section 14.3.4 above). ‘* Copy: This command allows you to copy selected records to the Clipboard for tansfer to your document of choice. © Save As: This command allows you to save any selected records to the file name and folder of your choice. © Exit: This is one way to close the DBViewer tool. New Brunswick Scientific Users Guide422, B. Use the Options dropdown memu: +to access the following commands: © Open BatchEvents: This command will open the BatchEvents data table for this batch. The BatchEvents table lists the time and description of any and all Alarm events that have occurred during the period logged. (See Section 13.3.1 fora sample table.) © Open ChangeRecord: This command will open the ChangeRecord data table for this batch. The ChangeRecord table lists any changes that have been made during the period logged and when each occurred. (See Section 13.2.1 for more information and a sample table.) © View Entire Database: xxxxx.bdb (where “xxxxx.bdb” is the name of the batch on display): Selecting this command will open a window labeled with the name of the batch (see sample window below). BioGommand Lite M1286-0060, Use*s Guide423 |G Batch vents |B BatchiD Bi LocTines, Loops [BhcurenStaus GE) MoyChanges [BEwors |BILcContig |BBLog age Blog Ai 1 GB Reporsetinss hicg.002 1 EB SttizeSetings [Blog Foan1 EE) SupvChangee In the left pane of this window are icons that represent every Data Log table available for this batch. These records are stored in a format compatible with the Microsoft Access” database management program. (See Chapter 13 for details on each Data Log table). As explained in Chapter 13 and at the beginning of this chapter, some of these tables will be empty of data because the features they represent are not used in BioCommand Lite 1. Double click on the icon for the desired database. The right pane will display the logged data Fa a a SBAunSetrgr — Ellog Nua iSanDegar |_| EE. D]Lagiae =| Baeisetny: logs 1 B)Uredccess |_|. 112789) Meacievers Ellon Pes! lUsetsDiage |_| 2 rDT98 [Biaecno Bloatow! Eve | 327/88 conccesetinge 4127798 [BchargeRecnd Lewes 178 [Bcurensisus — EvaCharges gree IB cnoe Botinedas = 7 Yas o iar leBtcconig Eh Onecnarses 4 eras Lea ADt Raparrene oe IBlioar 1 Gi RenotSetings triers JGhiog.002 1 el SterzeSetings See IBtonFoani — B)Swoxchnges [arenes Ld} ce “To widen the pane, place your cursor on any edge or corer of the windowpane; when the cursor becomes a double-headed arrow (<>), hold down the left mouse button and drag the edge out until the pane is the desired size New Brunswick Scientific, User's Guide424 3. Use the slider bars to scroll through the data records. 4. Click on X to close this window. C. Use ‘The Report Specification and Print Report functions are not used in BioCommand Lite the Tools dropdown menu: to access the following commands: Convert Database 2.0 to 3.0: If you have data that was logged and saved in 4FS-BioCommand (pre-BioCommand Lite), use this ‘command to convert that data so it can be read and used by BioCommand Lite. 1, When you click on Convert Database 2.0 to 3.0, a new window will open. 2. Click on the top {Browse} button to locate the old database that you need to convert 3. Double click on that database when you find it. It will appear in the “Source File:” edit box. 4, Click on the second {Browse} button to open a “Save As” screen for the destination and name of the converted database. 5. Click on {OK} to place this new destination & name string in the “Dest. File-” edit box 6. Click on {OK} to start the conversion process. BioGommand Lite @ M1286-0050 Users Guide125 * Repair a damaged Database: If you encounter a problem reading or using a database file, this tool may help you to fix the problem. 1. Click on Repair a damaged Database. A new window will open. 2. Use the {Browse} button to locate the problem database file. When you have selected it, it will appear in the edit box. 3. Click on {Repair}. - 4, Close the screen by clicking on {OK}. 14.4 More Batches The Database Viewer will also give you quick access to data for other, previously run batches. All data logged while the batch was running is available through the DBViewer. 1. In the “Existing Batches” window, either double click on “More...” or select ‘More...’ and then click on the {Open} button. ‘The “Open” window will appear, offering you a full choice of batch histories: van fase ol Sl oem Pears Siete gee eet oe jen bde Bite [sorom lest a 2b [Sleia Beas Bicicindas —Slrwu eb felest Beane loin eb v5 is] eBa.bdb ‘BleBod (aJElsBioRcp!.teb ia) Recipe b Pah at at Flo pame: Den Fin alype” FE) red Open se esconty To move a batch history into the “Existing Batches” window, double click on the batch of interest. Use the ‘More...’ selection and the “Open” window for as many batches as you would like to view. 4. When the desired batch is listed in the pane, select it, then click on the {OK} button. DBViewer’s data window will open. Refer back to Section 14.3 above. Naw Brunswick Scientific Users Guide126 THIS PAGE INTENTIONALLY BLANK ‘BioCommand Lite @ M1286-0050 Users Guide“Absolute Alarm, 60) ‘Active Batches. Resuming, 65 Adding Views, 99 ‘Alarms, 59, 80,93, 94, 108 Alarms Tab, 94 B Batch Definition of, 14 Deleting & di Deleting Views, 99 Ending, 102 Ending a 65 Functions of 79, Icon, 39 Modifying. 97, 102 New, 39.69, 95. 13 RestarvResume. 66, 69 Restaring/Resuming, 101. 102 Staring & 38, 41 ‘Terminate. 66 Terminating a 65 View. 93 Batch Manager Server, 114 Batch Summary, 25, 43.71, 80,93, 97 Batch Summary View. 45 Batch Wizard. 15.25.95 BatchE vets. 110 BioCommand Interface. 20, 2 < Cable Comections. 17 CAUTION ‘Symbol for. vi Changing @ Trend View. $6 Changing Data Logging Criteria, 102 Comm Port Setup. 20 ‘Communication Cabling. 18 Computer Setup of 11 Computer Operating Systems, 106 Computer Recommendation, 106 ‘Computer Requirements. 105 ComputerControllerts) Communication. 18 Controller ID Numbers Sample Retrieval of, 22 Controller IDs. 21 Controller Stas. 24 15 INDEX Controllers, 5, 6,34, 86, 105 Copying a Recipe, 90 Copyright Notice, vii Creating New Recipe, 32 : Creating a Trend View, 46 « D Data Logging Changing criteria for, 102 Interval of 14 Database Viewer Browse for More Batches, 117 Existing Batches, 115 Exporting Data to Excel, 119 Selecting Batch Data, 115 Starting the, 115 ‘To convert # 2.0 database to 3.0, 124 ‘To open BatchEvents, 122 ‘To open ChangeRecord, 122 To prim Repors, 124 ‘To repaira damages Database, 124 To specify Repors, 124 ‘To view Entire Database, 122 Deleting Views, 99 Diselaimer Notice. vi Displays. 5,6 Duplicating @ Recipe. 90 E Edit Funetion, 70, EFT, 42,43, 44, 49, 72, 84,96 Elapsed Fermentation Time. See EFT Exiting BioCommand, 65 F File Function. 69 File Name Extensions, 9 File Naming Conventions. 9 G Getting Started, 13, H Hardware Connections, 17 Setting up the, 19 Help Function, 77 New Brunswick Scientific Users Guide128 4 I International Offices List of ii K Keyboard Notation, 7 L License Agreement USS. Government Restricted Rights, 3 Log Interval, 14,83, 102 Loops Add/Change, 34, 35.85, 88 Alarms for, $9.94 Available, 35,36, 47. 60, 80. 86 Current, 36, 85, 88. 92 in Batch, 35. 36, 88, 93 Setpoints for. 97 Setup Options. 37 M Main Menu. 15, ‘Manual Conventions vi Menu Notation, 7 Meuse Notation. 7 Multi-Fermentor Adapter. 20. 22 N Navigating Through Windows, 8 NOTE Symbol for vi oO Object Naming Conventions, 9 Offline Data Entering. 72 ff.ine Data Function, 72 Mg Product Registration Number Obtaining a. 1 R Recipe Browse, 82 Copying a 90 Creating. 25 Creating a new, 25, 32 Data Pane, 83| Definition of. 14 Deleting a, 41 Description of, 33, 83 Duplicating a, 90 Editing, 79 File naming for, 29 Functions of, 73 ‘Naming 2.27, New, 49,69, 79, 82. 96 Renaming 2.27 Replace Loops in, 91. See Loops Save as, 81 View, 69,82. 96 Recipe Data Tab, 84 Recipe Tab, 81 é Restaning with Active Batghes, 6 Resuming a Batch, 66 Ss Select Comm Port, 20, ‘Setpoint Deviation Alarm. 60 Sespoints Changing, 97 Entering initial, 37 Setting initial, 90 Setting Alarms, $9 Shutting down BioCommand, 67 Specifications. 105 Starting BioCommand, 13, Starting BioCommand Screen, 14,25 System Requirements, 105, T Table of Contents. xi ‘Time Data, 83, 102 Toolbar. 15.16, 32.39, 77, 79, 80, 96 Toggling the, 71 Tools Function, 71 ‘Trend Graphs, 49 ‘Changing Background Color, $7 Changing Line Color. $7 Graphs Control Panel Tab, 55 Loops Controt Pane! Tab, $4 Main Control Pane! Tab, 5 Marking Specific Times, $3, Modifying, 56 ‘Moving through Time Records. 53 Styles Contol Panel Tab. 55 Trend View Modifying s. $6 Trend Views, 80 Troubleshooting, 103, Tun OFF Wizard, 15 ‘Tur On Wizard, 15 View Function, 70 Views Adding, 99 Deleting, 99 Views Tab, 93 BioCommand Lite @ M1286-0060 Users Guideey 129 — Symbol for, vi w Warranty WARNING Window Function, 78 New Brunswick Scientific User's Guide

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