TIU CHUN VIT NAM

TCVN 6600:2000
(ISO 6654: 1991)
THC N CHN NUI
XC NH HM LNG URE
Animal feeding stuffs
Determination of urea content
TCVN 6600: 2000 hon ton tng ng vi ISO 6654:1991
TCVN 6600: 2000 do Ban k thut tiu chun TCVN/TC/F17 Thc n chn nui bin son, Tng
cc Tiu chun o lng cht lng ngh, B Khoa hc Cng ngh v Mi trng ban hnh.
1. Phm vi p dng:
Tiu chun ny qui nh phng php o ph xc nh hm lng ure trong thc n chn
nui.
2. Tiu chun trch dn:
ISO 6498: 1983 Thc n chn nui - Chun b mu th
3. nh ngha:
Trong tiu chun ny p dng nh ngha di y.
Hm lng ure: Phn lng cht thu c khi xc nh theo qui trnh qui nh trong tiu chun
ny.
Phn lng ny c biu th di dng phn trm khi lng.
4. Nguyn tc:
Lm mt mu dung dch huyn ph ca mu th. Khuy dung dch ny v lc. Thm vo dch lc
dung dch 4-dimethyl-amino-benzaldehyde (4-DMAB) v o hp th quang hc trn my o
ph bc sng 420nm.
5. Thuc th:
Tt c thuc th u phi c cht lng tinh khit phn tch. Nc s dng phi l nc ct
hoc nc c tinh khit tng ng.
5.1. Than hot ho: Khng hp ph ure.
5.2. Dung dch 4-dimethyl-amino-benzaldehyde (4-DMAB) c chun b nh sau:
Ha tan 1,6g 4-DMAB trong 100ml etanol 96% (v/v), thm 10ml axt clohydric m c (p 20 =
1,19g/ml) v khuy u.
Dung dch c th gi ti a 2 tun.
5.3. Dung dch Carrez I:
Ho tan trong nc 24g km axetat dihydrat [Zn(CH 3COO)2.2H2O] v 3g axit axetic bng. Thm
nc ct cho 100ml v khuy u.
5.4. Dung dch Carrez II:
Ho tan trong nc 10,6g kali hexacyanoferrate (II) trihydrat (kali ferrocyanide trihydrat)
{K4[Fe(CN)6].3H2O}. Thm nc cho 100ml v khuy u.
5.5. Ure, dung dch chun cha 1g ure trong 1 lt.
6. Thit b, dng c:

S dng cc dng c thng thng trong phng th nghim v c bit l cc loi sau y:
6.1. My lc, c kh nng lm vic tc 30 - 40 vng/pht.
6.2. Ph k, thch hp cho vic o hp th bc sng 420nm v vi cuvt c chiu dy l
10mm.
6.3. ng nghim, kch thc 160mm x 16mm, c nt mi thu tinh.
6.4. Bnh nh mc, dung tch 100ml v 500ml.
6.5. Ni cch thu, c th khng ch nhit 20oC.
7. Ly mu:
Vic ly mu s c cp n trong mt tiu chun khc.
8. Chun b mu th:
Chun b mu theo ISO 6498.
9. Cch tin hnh:
9.1. Phn mu th:
Cn, chnh xc n 1mg, khong 2g mu th (iu 8).
i vi mu c hm lng ure ln hn 3% (m/m), c th gim phn mu th xung 1 g hoc
pha long dung dch mu (xem iu 9.2) nng khng vt qu 50mg ure/500ml.
i vi mu c hm lng ure thp, phn mu th c th tng ln sao cho dch lc thu c
phi trong v khng c mu.
9.2. Chun b dung dch mu th:
9.2.1. Chuyn phn mu th (9.1) cng 1g than hot ho (5.1) vo bnh nh mc 500ml (6.4).
Thm 400ml nc, 5ml Carrez I (5.3) v 5 ml dung dch Carrez II (5.4). Lc u bnh trn my lc
(6.1) trong 30 pht. nh mc n vch bng nc ct, lc u v lc qua giy lc nh tnh, tc
chm.
9.2.2. Nu dch lc vn c mu th chun b li dch lc nh iu 9.2.1 nhng tng thm lng
than hot ha.
9.3. Ln mu:
Dng pipt ht 5ml dch lc trong, khng mu (9.2) cho vo ng nghim (6.3), dng pipet ht
thm vo 5ml dung dch 4 - DMAB (5.2).
Lc u vo ng ng nghim trong ni cch thu 15 pht nhit 20 oC.
9.4. Mu trng:
Chun b mu trng song song vi mu kim tra, cc bc tin hnh tng t v lng cc
thuc th cho vo cng tng ng nh chun b mu th ch khc l khng c dch mu.
9.5. Chun b th chun:
9.5.1. Dng pipt ln lt ht 1ml, 2ml, 4ml, 5ml v 10ml dung dch ure chun (5.5) vo 5 bnh
nh mc 100ml (6.4). nh mc n vch bng nc ct. Nh vy 1ml dung dch chun cha
10g, 20g, 40g, 50g v 100g ure.
9.5.2. T cc dung dch chun ny (9.5.1) dng pipt ht chnh xc ln lt mi bnh 5ml cho
vo ng nghim (6.3). Thm vo mi ng nghim 5ml thuc th 4-DMAB (5.2) v lc u. o
hp th ca cc dung dch bc sng 420nm trn my o ph k (6.2). Hiu chnh vi dung
dch chun 5ml thuc th 4 - DMAB v 5ml nc.
9.5.3. Xy dng th chun vi trc tung l cc gi tr hp th v trc honh l nng ure
tnh theo g/ml.

9.6. o ph:
Chuyn dung dch mu th chun b theo iu 9.3 vo cuvt v o hp th trn my ph
k bc sng 420nm, hiu chnh vi mu trng (9.4).
Ch thch - Nu mu cha cc hp cht nit nh cc axit amin, th tin hnh o hp th
bc sng 435nm.
9.7. S php xc nh:
Tin hnh xc nh hai php xc nh cc phn th trn cng mt mu th.
10. Biu th kt qu :
Hm lng ure c trong mu th, tnh theo phn trm khi lng, c biu th bng cng thc
sau :

C
20 xm
Trong :
C l lng ure c trong dung dch lc ca mu th c xc nh t ng chun (9.5.3), tnh
bng g/ml.
m l khi lng mu th (9.1), tnh bng g.
11. Bo co kt qu:
Trong bo co kt qu phi ghi r phng php s dng v kt qu thu c. Bo co cng
phi cp n nhng chi tit thao tc khng c nu ra trong tiu chun ny hoc c php
la chn, cng vi cc chi tit ca bt k yu t no nh hng ti kt qu.
Bo co phi bao gm tt c cc thng tin cn thit nhn bit c ton din mu th.