EXERCISE NO. 4: PRACTICAL EXAM 1 Staining and Use of the Microscope 1.0 OBJECTIVES At the end of the exercise, the student must be able to: 1.1 Prepare stained slide for microbial examination; 1.2 Use a bright field light microscope to view and interpret a stained slide; 1.3 Use aseptic techniques during microbiological work; 1.4 Properly use and operate the Bunsen burner; 1.5 Practice safety precautionary measures during microbiological work. 2.0 PROCEDURE 24 22 23 24 25 26 27 Obtain the unknown bacterial culture from your instructor. List down all the materials required for the exercise in your record sheet. Prepare the flow diagram of the staining procedure you are tasked to perform, Gather all the materials needed and set-up your work station. Perform the staining procedure assigned. Set up the bright field light microscope and focus the stained slide using the HPO, Record and interpret your results, Call your instructor to validate your results Properly return the microscope.EXERCISE NO. 4: PRACTICAL EXAM I Staining and Use of the Microscope Record Sheet Family Name First Name MI Unknown Tube # List of materials: Flow diagram: Operation of bunsen burner (3 pts) Staining procedure (3 pts) Demonstration of aseptic technique (4 pls) Handling and use of microscope (5 pts)