BD CSampler Software User Guide 3 VALIDATING THE PERFORMANCE OF THE BD ACCURI C6 FLOW CYTOMETER Perform a validation of the system at least once each day of use. This ensures that the flow cytometer is working properly before running experimental samples. Using the same BD CSampler Software file each day allows identification of trends over time. When collecting validation data, BD CSampler Software advances to the. next empty well in row A-B (for 8-peak beads) or C-D (for 6-peak beads). Include the date in the Sample Naming Field for each day of validation. Start a new validation bead file when all data wells are full. Reagents required: ‘Spherotech 8-Peak Validation Beads (PN 653144, supplied with the initial flow cytometer ‘Spherotech 6-Peak Validation Beads (PN 653145, supplied with the initial flow cytometer + Sheath fluid: Deionized, filtered water (0.2 ym filter) plus Bacteriostatic Concentration Solution (PN 653156, supplied with the initial flow cytometer shipment) Running Validation Beads (Daily Start-Up) Setup: * Verify that the Bead Template has been copied to the BD CSampler Software computer. The file is located on the BD CSampler Software CD or flash drive and is on the BD Accuri website (ntto/www.bdbiosciences.com/support/resources/accuri/index.isp/). NOTE: The template is set up for the 24-tube rack only. * Open BD CSampler Software. * Select File > Open Workspace or Template. * Inthe Open dialog box, browse to the location of the bead template file and open the file. If adding to an established BD CSampler Software bead file, browse to the location of the file. 7820099-01 Rev-2 "7