Keto Acid Concentration (M)

Initial Velocity (CO2/2min) CO2/min

2.5
0.0000098 0.0000049
1 8.33333333333333E-006 4.1667E-006
0.714
0.00000695 3.4750E-006
0.526 6.16666666666667E-006 3.0833E-006
0.25 4.26666666666667E-006 2.1333E-006

Vmax
5.67952700899069E-006 mM/S
Km/Vmax
74074
Km

500000
450000

f(x) = 74073.9326173176x + 176071.306879549

R = 0.994341311

400000
0.4207052837 (M)
350000
300000
250000
200000
150000
100000
50000
0
0

LINEWEAVER BURKEE

0.5

1.5

2.5

3.5

4.5

SEC/ MILIMOLAR CO2

1/KAC
204081.632653061
0.4 204081.633
240000
1
240000
287769.784172662 1.40056022 287769.784
324324.324324324 1.90114068 324324.324
468750
4
468750

76x + 176071.306879549

2.5

Column F
Linear (Column F)
Linear (Column F)

3.5

4.5

Keto Acid Conc M

Initial Velocity
10^3 vo/s
v0 (mM/S)
2.5
4.9E-009 1.9600E-009 0.0000049
1
4.16666666666667E-009 4.1667E-009 4.1667E-006
0.714
3.475E-009 4.8669E-009 3.4750E-006
0.526
3.08333333333333E-009 5.8619E-009 3.0833E-006
0.25
2.08333333333333E-009 8.3333E-009 2.0833E-006

Vmax= 6x10-6 mM/S

Km =454 mM

Eadie Hoffstee Plot

0.000006
0.000005
f(x) = - 453.9994529813x + 5.82880669870375E-006
R = 0.9817519935

0.000004

Column D

0.000003

Linear (Column D)
0.000002
0.000001
0
0

0.000000005

0.00000001

75E-006

Column D
Linear (Column D)

Keto conc (M) Initial Velocity

IVS *2/60 (M/S)
2.5
0.588
0.0000049
1
0.5 4.16667E-006
0.714
0.417 0.000003475
0.526
0.37 3.08333E-006
0.25
0.256 2.13333E-006

M=1/Vmax
4 x10-7
Vmax
5.7728721193E-006 M/s
B=Km/Vmax
80000000
Km= B* Vmax
461.8297695469 mM

Hanes Plot

2.5 510204.082
1
240000
0.714 205467.626
0.526 170594.595
0.25
117187.5

600000
500000

f(x) = 173223.834867674x + 75813.3732273674

R = 0.9985459527

400000

Column E

300000

Linear (Column E)
200000
100000
0
0

0.5

1.5

2.5

Column E
Linear (Column E)

Keto conc (M)Initial

2.5
1
0.714
0.526
0.25

VelocityIVS *2/60 (M/S)

0.588
1.96E-008
0.5 1.6667E-008
0.417
1.39E-008
0.37 1.2333E-008
0.256 8.5333E-009

vmax

vo1
1
1
1
1
2
2
2
3
3
4

2
3
4
5
3
4
5
4
5
5

0.588
0.588
0.588
0.588
0.5
0.5
0.5
0.417
0.417
0.37

km
0.62
0.631
0.647
0.666
0.687
0.698
0.703
0.733
0.82
0.994

Median method

0.332
0.356
0.366
0.394
0.421
0.466
0.466
0.49
0.639
0.988

vmax

0.6925
5.77E-6 mM/s

km

0.4435
0.4435 M

vo2

7E-6 mM/s

S1
0.5
0.417
0.37
0.256
0.417
0.37
0.256
0.37
0.256
0.256

S2
2.5
2.5
2.5
2.5
1
1
1
0.714
0.714
0.526

S2-S1
1
0.714
0.526
0.25
0.714
0.526
0.25
0.526
0.25
0.25

S2V1-S1V2 Vmax
-1.5
-0.662 0.66616314
-1.786
-0.622668 0.70329623
-1.974
-0.615712 0.69750702
-2.25
-0.493 0.68699391
-0.286
-0.06
0.99385
-0.474
-0.107 0.81953271
-0.75
-0.131 0.73282443
-0.188
-0.044838 0.64691824
-0.464
-0.078534 0.63071954
-0.276
-0.042156 0.62014233

v2-v1

Km
-0.088 0.33232628
-0.171 0.49020505
-0.218 0.46559106
-0.332 0.42089249
-0.083
0.9877
-0.13 0.63906542
-0.244 0.46564885
-0.047 0.39367296
-0.161 0.36593705
-0.114 0.35560774