Correlation Between

Hypoxia Inducible Factor

(HIF)-1 Expression with
Cell Death in Colorectal
Cancer
Nungky Kescandra
20100310122
Faculty of Medicine and Health Science
Muhammadiyah University of Yogyakarta

Background
Colorectal cancer is the 3rd most common

cancer in men and the second in women

worldwide epidemiology in 2008.
World 1.234.000 incidences with the

mortality about 608.000 (IARC, 2008).

Indonesia in 2008, there were about 33.379

incidences with mortality range around 25.253

(IARC, 2008).

TUMOR

HYPOXIA

HIF-1

NEKROSIS

Problem Definition
What is the relation between Hypoxia
Inducible Factor (HIF)-1 with cell death in
colorectal cancer?

Literature Review

Nature.com

Pathophysiology of Cancer
Colorectal
Cancer
Polyp
Colorectal
Mucosa
Polyp hyperplasia
Polyp
adenomatous

Robbins & kumar

Buku Ajar Patologi

Staging of Colorectal Cancer

http://www.hopkinscoloncancercenter
.org/

HIF-1
Hypoxia-Inducible Factor (HIF)-1 is a protein

that made of helix loop -heliks-Per-Arnt-Sim

(bHLH-PAS) substances
Protein ini merupakan penentu dari bentuk

dan aktivitas transkripsi dari HIF-1 yang

merupakan pengatur utama dari homeostasis
oksigen dan berperan penting dalam proses
fisiologi dan patologi adaptasi terhadap
tekanan oksigen rendah (hipoksia).

Regulation of HIF-1

Semenza,

(Semenza,
2003)

Cell Death
Apoptosis : the death of a cell that already has

been programmed and also has such an ability to

suicide itself through various cellular signals
pathways. Apoptosis didnt cause inflammation
and didnt harm another cells around (Edinger, &
Thompson, 2004).
Nekrosis : the death of a cell that unprogrammed,

in pathologic ways, and believed as trauma or

hypoxia condition result and irreversible (Zong, &
Thompson., 2006).

Hypothesis
There is relation between HIF-1 expression

with cell death in colorectal cancer

RESEARCH METHODOLOGY
Research
Design

cross sectional Nonexperimental

Research
Subject

patients are examined or transmit

the colorectomy to Cito Pathology
Laboratory in January 2011 until May
2012.

Inclusion
Criteria

Adenocarcinoma (operation) and

another Pathological Anatomy
examination from january 2011
may 2012

Exclusion
Criteria

Adenocarcinoma Biopsy

Variable
Independent:
HIF-1

Nominal

Variable
Variable
Dependent:
Cell Death

Nominal

Rumus Sampel

Work
WorkProcedures
Procedures

Microscopi
c

HE
Staining

IHC
Staining

Analysis

Data Analysis
Data yang dikumpulkan kemudian

diuji menggunakan Chi-Square .

Thank You