SUPEROXIDE

DISMUTASE (SOD)
COLORIMETRIC
ACTIVITY KIT

BACKGROUND
In the resting state, the balance between
antioxidants and oxidants is sufficient to
prevent the disruption of normal physiologic
functions
Either increases in oxidants or decreases in
antioxidants can disrupt this balance giving
rise to elevated levels of reactive
oxygen
species (ROS)

ROS are controlled by antioxidant

enzymes
and small molecule
antioxidants SOD

PRINCIPLE
SOD
ACTIVITY
KIT

To quantitatively measure
SOD
activity in a variety
of samples
Use
Calibrator
Samples diluent
in specially
colored sample
The diluent
substrate is added
followed by Xanthin
Oxidase Reagent and
incubated at room
temperature
for
Increasing
levels
of20
SOD
minutescauses a
in the samples
decrease in superoxide
concentration and a
reduction in yellow

COMPONENTS

Clear 96 Well Half Area Plates 2 Plates

Superoxide Dismutase Calibrator 1 vial
Xanthine Oxidase Buffer 6 mL
Xanthine Oxidase Concentrate 225 L
Assay Buffer 50 mL
Substrate Diluent 12 Ml
Substrate Concentrate 1.1 mL

CELL SUSPENSIONS AND

ADHERENT CELLS

CELL SUSPENSIONS AND

ADHERENT CELLS

TISSUE SAMPLES
Wash tissue thoroughly with ice cold PBS prior to processing.

PLASMA SAMPLES

ERYTHROCYTES

3.
4.
2.
1.Centrifuge
RBCs should
atbe
10,000
diluted
x gatfor
least
15 minutes
1:100 prior
at 4C
to running
to remove
in the
d

SERUM SAMPLES
1

ASSAY PROTOCOL
Pipet

Add 25 L of the

CALCULATION OF RESULT