Why to study light absorption?

Extracted electrons = f(Reaching photons)

Emission spectrum (LED, solar, any combination)
Absorption spectrum in vivo
Photoadaption (NPQ, state transitions)
Fluorescence
Energy efficiency
Energy Photosystem balance

 Emission spectrum
LED

Fluorescent lamp

Solar irradiance

 Absorption spectrum (WT) Spectrophotometer

 Absorption spectrum (Olive) Spectrophotometer

 Absorption spectrum (WT) White LED

Absorptium spectrum
(components)
Absorption spectrum

Lamp emission spectrum

 Absorption spectrum (WT) Orange LED,

590 nm

 Light absorption results

Chl a

WT
Spectrophotomet
er

Photoprotective C.

Photosynth. C.

Phycocyanin

Allophycocyanin

25,5

8,4

10,8

17,8

37%

12%

15%

1,9

0,4

0,5

WT solar light

26%

2,9

6%

1,1

7%

1,1

WT blue light

42%

0,1

15%

0,0

N(440 nm, 10nm)

WT orange light

63%

20%

0,0

N(440 nm, 10nm)

Olive
Spectrophotomet

10%

0%

WT lamp

0,0

7,3

26%
71%

10%
29%

3,6

0,9

49%
81%
1,4

12%
19%
0,5

15%

0,0

20%
73%
0,0

7%
27%
0,0

13%

0,0

3%

2%

76%
0,0

1%

74%
84%

24%
0,0

14%

16%

 Blue light absorption

Cyclic flow

PSII

PQ

Fluorescence?
PSI Heat? Blue = State 1
Cyclic flow (10-50%)

5-10% Chl a 0 E (dark) PQ

90-95%
red 60%
Chl a
Limiting step:100 E (LL) Limiting
PQred 10%
step:
Qa- + Qb- (ms)1000 E (HL)Fb-PQ
+ red
Fd 0%
(s)

 Light affects cell behaviour

Electron flow affected

 Equations
Rod + hv Rod*

Rod* + APC Rod + APC*

(J/s)

 Energy costs
Growth associated (building blocks,
polymerisation)
Basal (osmotic pressure)
Olive (saving prophycobilin costs)
Photoinhibition spilling