Pamantasan ng Lungsod ng Maynila

College of Engineering and Technology

Department of Chemical Engineering

EXTRACTION AND PULVERIZATION OF DRIED SAMPAGUITA FOR THE

PRODUCTION OF TITRATION pH INDICATOR

CONCERNS ON USE OF SYNTHETIC INDICATORS

Today synthetic indicators are the choice of acid-base titrations. Commercial
indicators are expensive and some of them have toxic effects on users and can also
cause environmental pollution. For these reasons there has been an increasing
interest in searching for alternative sources of indicators from natural origins. These
alternatives would be cheaper, more available, simple to extract, less toxic to users
and environmentally friendly.
Many substances around us that can be used as an indicator of acid and alkali. For
example, leaf, flower, turmeric, mangosteen skin, and purple cabbage. This is
because the solution of these materials give a different color on acid, alkali and
neutral solutions.

SAMPAGUITA AS FLAVONOID AND TANNIN SOURCE

According to Abugri et. al. (2012), total flavonoids (TF) and condensed tannin (CT)
from the crude leaves might be the major reasons for the activity of extracts as an
indicator for simple acid-base titration. Kunhachan et al. (2012) made a
chromatographic analysis on the components of sampaguita.

Fulugan, Ceazar Justine L. | Mendoza, Michelle B. | Redublo, Anne Paulinne P. | Sim,

Tristan James G.

Pamantasan ng Lungsod ng Maynila

College of Engineering and Technology
Department of Chemical Engineering

GENERAL PROCEDURE FOR THE SOLVENT EXTRACTION OF NATURAL

INDICATORS
Flowers were cleaned by distilled water and petals of these flowers were kept in
strong sunlight until they get completely withered. The dried petals were grinded
into fine powder with a mechanical blender and then triturated in mortal and pestle
and the resulting solution was filtered through muslin cloth. The resulted aqueous
extract was used as natural indicator for acidimetric and alkalimetery. The extract
was preserved in light closed container and stored away from direct sunlight.
Following the similar process methanolic extract of dried pulverized petals was
prepared, filtered and used as natural methanolic indicator. Reagents and
volumetric solutions were prepared as per standard.

Fulugan, Ceazar Justine L. | Mendoza, Michelle B. | Redublo, Anne Paulinne P. | Sim,

Tristan James G.