Hydrolysis of 4-NPP catalysed by alkaline phosphatase

Reactions (4 min) were 1.250 mL in total, with 2 mM 4-NPP in 0.5 M Tris, pH 9.2, excepting variations as noted
Each datum is the mean of 3 independent measurements of A400 for the time indicated.

time

Standard
Reaction

No E

phosphate

pH 7.2

Mg2+
supplement

EDTA

Mg2+ rescue

0.000

0.000

0.000

0.000

0.000

0.000

0.000

0.087

0.001

0.010

0.014

0.131

0.007

0.089

0.174

0.002

0.018

0.029

0.263

0.014

0.179

0.262

0.002

0.029

0.042

0.396

0.021

0.267

0.346

0.002

0.038

0.056

0.526

0.027

0.356