AL STUDENT EDITION i rinciples of Instrumental Analysis oe te -10 The data in the table below were obtained during a colorimetric determination of ‘glucose in blood serum, Glucove Absorbance, al ‘own 430 204 oa osm 0.704 (a) Assuming a linear relationship, find the least-squares estimates of the slope sand intercept. (b) Use the LINEST function in Excel to find the standard deviations of the v slope and intercept.’ What is the standard error of the estimate? (©) Determine the 95% confidence intervals for the slope and intercept (a) A serum sample gave an absorbance of 0.350. Find the glucose concentration ind is standard deviation, [3g] 114 Exacity $.0.mL aiquots ofa solution containing phenobarbital were measured ME into 50.00-mL. volumetric Masks and made basic with KOH. The following vol tues ofa standard solution of phenobarbital containing 2.000 jegimL of pheno- barbital were then introduced into wach Mask and the mixture was diluted t0 volume:0,000, 0.500, 1.00 1.50, and 2.00 mL. The Nuoreseence of each ofthese solutions was measured witha fuorometer which gave values of 3.26, 480,61, 802, and 936, respectively (a) Plot the data. *(b) Using the plot from (a), calculate the concentration of phenobarbital in the unknown *(c) Derive a least-squares equation for the data. ‘*(d) Find the concentration of phenobarbital from the equation in (c). (©) Calculate a standard deviation forthe concentration obtained in (a).11 stangata sorution Was put nrougn appropriate ctutions (0 give the concentra tions of iron shown below. The iron(II)-1.10.phenanthrotine complex was then formed in 25.0-mL aliquots of these solutions, following which each was diluted 10 50.0 mL. The following absorbances (1,00-em cells) were recorded at 510 nm: Fe(il) Conceotation in Original Solutions, ppm Ase ie Diet too a 160 a 240 asr 320 per 400 ear (2) Plot a calibration curve [rom these data (b) Use the method of least squares to find an equation relating absorbance and the concentration of iron(II). (©) Calculate the standard deviation of the slope and intercept | 14-12 The method developed in Problem [4-11 was used forthe routine determination of ron in 25.0-mL aliquots of groundwater. Expres the concentration (8s porn Fe) insamples tha yielded the accompanying absorbance dat (1m cel Calculate the relative standard deviation of the result. Repeat the calculation assuming the absorbance data are mican of hee measurements (a) 0143 (@) 0068 (e) 1512 (b) 0678 (@) L009 (0546 ]X| 14-13 Copper(t1) forms a I: | complex with the organic complexing agent R in acidic medium. The formation ofthe complex can be monitored by spectrophotometry 21 480 nm, Use the following data collecied under pscudo-firs-order conditions tw construct a calibration curve of rate versus concentration of R. Find the con- centration of copper(I) in an unknown whose rate under the same cor wan 62 x 108A 57! coos ppm RateAst 30 36x10" 50 54x10 10 79x10 90. 103 x 10? adenine dinucleotide (NADH) san imports Te has an abyorption maxinaumy of Mn 4 i sotutions of NADH gave the follows ov of nicotinamide tate semnt COLNZYINC. dg one Mhuorescen intensities: {465 nim, Standard oan 300 0 «500 090 0.700 oxo(2) Construct a spreadsheet and use it to draw a calibration curve for NADH, *(b) Find the least-squares slope and intercept for the plot in (a). (6) Calculate the standard deviation ofthe slope and the standard deviation about regression for the curve. *(d) An unknown exhibits a relative Auorescence intensity of 12.16, Use the spreadsheet to calculate the concentration of NADH. *(e) Calculate the relative standard deviation forthe result in part (2), *(0) Calculate the relative standard deviation for the result in part (d) if result (0f 7.95 was the mean of three measurements, J1¢]_ 15-8 ‘The volumes ofa solution containing 1.10 ppm of Zn2* shown inthe table were pipetted into separatory funneis, cach containing 5.00 ra. of an unknown zine solution, Each was extracted with three S-mL aliquots of CCI, containing an ex- cess of 8. hydroxyquinoline, The extracts were then diluted to 25.0 mL and their fluorescence measured with a fuorometer. The results were the following Volume Std. Fluorometer Zn''smL Reading 000 612 400 1116 800 1368 R00 2064 (a) Construct a working curve from the data, (b) Calculate a linear least-squares equation for the data. (©) Calculate the standard deviation of the slope and intercept and the standard deviation about regression, (@) Calculate the concentration of zine in the sample. (€) Calculate a standard deviation for the result in part (a) "159 Quinine in a 1.664-g antimalarial tablet was dissolved ia sufficient 0.10 M HCL 10 ‘give 500 mL. of solution. A 20.00-mL aliquot was then diluted to 100.0 mL. with the acid, The Auorescence intensity for the diluted sample at 347.5 nm provided a reading of 245 on an arbitrary scale, A standard 100-ppm quinine solution re istered 125 when measured under conditions identical to those for the diluted sample, Calculate the mass in milligrams of quinine in the tablet ‘The determination in Problem 15-9 was modified to use the standard-addition thod. In this case, a 4.236-g tablet was dissolved in sufficient 0.10 M HCI to ive 1.000 L. Dilution of a 20.00-mL aliquot to 100 mL yielded a solution that ‘gave a reading of 448 at 347.5 nm, A second 20,00-mL aliquot was mixed with 10.0 mL of 50-ppm quinine solution before dilution 10 100 mL. The Muorescence intensity ofthis solution was 525. Calculate the percentage of quinine in the tablet.