Antibiotics inhibit bacteria by inhibiting specific steps in the bacterial reproduction
process. Penicillin and other beta-lactam antibiotics act by binding to and inhibiting proteins in the bacterial Cell wall called penicillin-binding proteins (PBPs). Different beta-lactam drugs have varying affinities for these PBPs, accounting for potential differences in activity among the beta-lactam drugs against particular bacteria. Fluoroquinolone antibiotics are derivatives of the urinary tract agent nalidixic acid. Bind to bacterial DNA gyrase and topoisomerase IV, interfering with bacterial DNA synthesis. Aminoglycosides and tetracyclines act by binding to the bacterial 30S ribosome, inhibiting bacterial protein synthesis. Macrolide antibiotics inhibit bacterial protein synthesis by reversibly binding to the 50S ribosomal subunit. Sulfonamide antibiotics inhibit steps in bacterial folic acid synthesis.